| Information Item | Value |
|---|---|
| Dataset Name | Hol_tfa1_ts: temperature sensitive TFA1 mutant data |
| Dataset Number | 15 |
| Short Description | expression levels as affected by temp sensitive mutation of TFA1 |
| Source URL | http://www.wi.mit.edu/young/expression.html |
| Reference | Holstege FC; Jennings EG; Wyrick JJ; Lee TI; Hengartner CJ; Green MR; Golub TR; Lander ES; Young RA, Dissecting the regulatory circuitry of a eukaryotic genome, Cell 1998 Nov 25;95(5):717-28 |
| Strains | Z889 (W303 genetic background) MATalpha, ade2-1, leu2-3,112, trp1-1, ura3-1, can1-100, tfa1::ADE2 [ptfa1-21(AMP, CEN, TRP1 HA-tfa1-21)] |
| Conditions | YPD Medium, strain grown to an OD of about 0.6 at permissive temp (30 or 24), harvested after 45 minutes at 37 or 38 |
| Date Added to ExpressDB | Feb 9 1999 2:47:52:480PM |
| Number of Measures on ExpressDB | 10 (here to download dataset and view measure details) |
| Long Description | Data Set loaded by Wayne. wt strain is Z888 (MATalpha, ade2-1, leu2-3,112, trp1-1, ura3-1, can1-100, tfa1::ADE2 [pSK492(AMP, CEN, TRP1, HA-TFA1)]) Medline Abstract - Genome-wide expression analysis was used to identify genes whose expression depends on the functions of key components of the transcription initiation machinery in yeast. Components of the polymerase II holoenzyme, the general transcription factor TFIID, and the SAGA chromatin modification complex were found to have roles in expression of distinct sets of genes. The results reveal an unanticipated level of regulation which is superimposed on that due to gene-specific transcription factors, a novel mechanism for coordinate regulation of specific sets of genes when cells encounter limiting nutrients, and evidence that the ultimate targets of signal transduction pathways can be identified within the initiation apparatus. |
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Copyright (c) 2006 by Wayne Rindone and the President and Fellows of Harvard University