Information Item | Value |
---|---|
Dataset Name | Spe_cln3: Spellman Cln3 experiments |
Dataset Number | 24 |
Short Description | CLN3 induction experiments |
Source URL | http://cellcycle-www.stanford.edu/ |
Reference | Spellman, Paul T. et al, Mol.Biol.Cell 9:3273 (1998) |
Strains | #31 genotype MATa leu2 ura3 cln1::HIS3 cln2::TRP1 cdc34-2ts ura3-GAL-CLN3 |
Conditions | Two experiments, using two hybridizations in which CLN3 expression was induced without inducing cell cycle progression, 40 minute sample for experiment 1 (cln3-1), 30 minute sample for experiment 2 (cln3-2) |
Date Added to ExpressDB | Apr 8 1999 12:01:58:386PM |
Number of Measures on ExpressDB | 55 (here to download dataset and view measure details) |
Long Description | cln3- cells were arrested in late G1 with cdc34-2, then CLN3 expression induced in experimental portion by addition of galactose, compared with control sample taken at time of galactose addition. Data Set loaded by Wayne Abstract from article: We sought to create a comprehensive catalog of yeast genes whose transcript levels vary periodically within the cell cycle. To this end, we used DNA microarrays and samples from yeast cultures synchronized by three independent methods: alpha factor arrest, elutriation, and arrest of a cdc15 temperature-sensitive mutant. Using periodicity and correlation algorithms, we identified 800 genes that meet an objective minimum criterion for cell cycle regulation. In separate experiments, designed to examine the effects of inducing either the G1 cyclin Cln3p or the B-type cyclin Clb2p, we found that the mRNA levels of more than half of these 800 genes respond to one or both of these cyclins. Furthermore, we analyzed our set of cell cycle-regulated genes for known and new promoter elements and show that several known elements (or variations thereof) contain information predictive of cell cycle regulation. A full description and complete data sets are available at http://cellcycle-www.stanford.edu/ |
Please contact Wayne Rindone for more information, or with any questions, comments, or concerns.
Copyright (c) 2006 by Wayne Rindone and the President and Fellows of Harvard University