| Information Item | Value |
|---|---|
| Dataset Name | Spe_clb2: Spellman Clb2 experiments |
| Dataset Number | 25 |
| Short Description | CLB2 induction experiments |
| Source URL | http://cellcycle-www.stanford.edu/ |
| Reference | Spellman, Paul T. et al, Mol.Biol.Cell 9:3273 (1998) |
| Strains | #245 genotype W303a clb1::URA3 clb2::LEU2 clb3::TRP1 clb4::HIS3 GAL-CLB2 |
| Conditions | Two experiments, using two hybridizations in which CLB2 expression was induced without inducing cell cycle progression, 40 minute samples for experiment 1 (clb2-1) and experiment 2 (clb2-2) |
| Date Added to ExpressDB | Apr 9 1999 3:20:23:350PM |
| Number of Measures on ExpressDB | 55 (here to download dataset and view measure details) |
| Long Description | clb2- cells were arrested in M with nocodazole, then CLB2 expression induced in experimental portion by addition of galactose, compared with control sample taken at time of galactose addition. Data Set loaded by Wayne Abstract from article: We sought to create a comprehensive catalog of yeast genes whose transcript levels vary periodically within the cell cycle. To this end, we used DNA microarrays and samples from yeast cultures synchronized by three independent methods: alpha factor arrest, elutriation, and arrest of a cdc15 temperature-sensitive mutant. Using periodicity and correlation algorithms, we identified 800 genes that meet an objective minimum criterion for cell cycle regulation. In separate experiments, designed to examine the effects of inducing either the G1 cyclin Cln3p or the B-type cyclin Clb2p, we found that the mRNA levels of more than half of these 800 genes respond to one or both of these cyclins. Furthermore, we analyzed our set of cell cycle-regulated genes for known and new promoter elements and show that several known elements (or variations thereof) contain information predictive of cell cycle regulation. A full description and complete data sets are available at http://cellcycle-www.stanford.edu/ |
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Copyright (c) 2006 by Wayne Rindone and the President and Fellows of Harvard University