Expression Data Set 25 details

Information Item Value
Dataset Name Spe_clb2: Spellman Clb2 experiments
Dataset Number 25
Short Description CLB2 induction experiments
Source URL
Reference Spellman, Paul T. et al, Mol.Biol.Cell 9:3273 (1998)
Strains #245 genotype W303a clb1::URA3 clb2::LEU2 clb3::TRP1 clb4::HIS3 GAL-CLB2
Conditions Two experiments, using two hybridizations in which CLB2 expression
was induced without inducing cell cycle progression, 40 minute samples for experiment
1 (clb2-1) and experiment 2 (clb2-2)
Date Added to ExpressDB Apr 9 1999 3:20:23:350PM
Number of Measures on ExpressDB 55 (here to download dataset and view measure details)
Long Description clb2- cells were arrested in M with nocodazole, then CLB2
expression induced in experimental portion by addition of galactose, compared
with control sample taken at time of galactose addition.

Data Set loaded by Wayne

Abstract from article:

We sought to create a comprehensive catalog of yeast genes whose
transcript levels vary periodically within the cell cycle. To this
end, we used DNA microarrays and samples from yeast cultures
synchronized by three independent methods: alpha factor arrest,
elutriation, and arrest of a cdc15 temperature-sensitive mutant. Using
periodicity and correlation algorithms, we identified 800 genes that
meet an objective minimum criterion for cell cycle regulation. In
separate experiments, designed to examine the effects of inducing
either the G1 cyclin Cln3p or the B-type cyclin Clb2p, we found that
the mRNA levels of more than half of these 800 genes respond to one or
both of these cyclins. Furthermore, we analyzed our set of cell
cycle-regulated genes for known and new promoter elements and show
that several known elements (or variations thereof) contain
information predictive of cell cycle regulation. A full description
and complete data sets are available at

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