| Information Item | Value |
|---|---|
| Dataset Name | Barak Cohen yap1 and yap2 knockouts with peroxide and cadmium added |
| Dataset Number | 36 |
| Short Description | Summaries of multiple gene chip experiments, wt, yap1 and yap2 knockouts and combinations with various treatments |
| Source URL | http://arep.med.harvard.edu/ExpressDB/EDS36/ |
| Reference | Discrimination between the Yap1p and Yap2p transcriptional networks using microarray analysis, Barak A Cohen, Yitzhak Pilpel, Robi D Mitra, and George M Church |
| Strains | wildtype is FY1351, genotype ura3delta0 lys2delta0 leu2delta0 yap1 deletion strain is BCY06, genotype yap1::URA3 ura3delta0 lys2delta0, leu2delta0 there are also yap2 knockouts and a strain with both knockouts |
| Conditions | minimal medium, minimal plus peroxide, and minimal plus cadmium |
| Date Added to ExpressDB | Feb 17 2000 1:16:56:130PM |
| Number of Measures on ExpressDB | 21 (here to download dataset and view measure details) |
| Long Description | Data Set loaded by Wayne There is a separate summary series for each strain and treatment combination. These include wild type control, with peroxide and with cadmium, yap1 knockout control and with peroxide and with cadmium, yap2 knockout control and with peroxide and with cadmium, double knockouts control and with peroxide, wild type and fold change numbers from wild type control for each strain and treatment. Ohno (Ohno, S. (1970) in Evolution by Gene Duplication, (Springer, New York)) proposed that gene duplication with subsequent divergence of paralogs could be a major force in the evolution of new gene functions. In practice the functional differences between closely related homologs produced by duplications can be subtle, and difficult to separate experimentally. Here we show that DNA microarrays can distinguish the functions of two closely related homologs from the yeast Saccharomyces cerevisiae, Yap1p and Yap2p. Although Yap1p and Yap2p are both bZIP transcription factors involved in multiple stress responses and are 88% identical in their DNA binding domains, our work shows that these proteins have non-overlapping functions. Yap1p controls a set of genes involved in detoxifying the effects of reactive oxygen species, whereas Yap2p controls a set of genes over represented for the function of stabilizing proteins. In addition we show that the binding sites in the promoters of the Yap1p dependent genes differ from the sites in the promoters of Yap2p dependent genes and we validate experimentally that these differences are important for regulation by Yap1p. We conclude that while Yap1p and Yap2p may have some overlapping functions they are clearly not redundant and, more generally, that DNA microarray analysis will be an important tool for distinguishing the functions of the large numbers of highly conserved genes found in all eukaryotic genomes. |
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Copyright (c) 2006 by Wayne Rindone and the President and Fellows of Harvard University