|Dataset Name||Barak Cohen yap1 and yap2 knockouts with peroxide and cadmium added|
|Short Description||Summaries of multiple gene chip experiments, wt, yap1 and yap2 knockouts and combinations with various treatments|
|Reference||Discrimination between the Yap1p and Yap2p transcriptional networks using microarray analysis,
Barak A Cohen, Yitzhak Pilpel, Robi D Mitra, and George M Church
|Strains||wildtype is FY1351, genotype ura3delta0 lys2delta0 leu2delta0
yap1 deletion strain is BCY06, genotype yap1::URA3 ura3delta0 lys2delta0, leu2delta0
there are also yap2 knockouts and a strain with both knockouts
|Conditions||minimal medium, minimal plus peroxide, and minimal plus cadmium|
|Date Added to ExpressDB||Feb 17 2000 1:16:56:130PM|
|Number of Measures on ExpressDB||21 (here to download dataset and view measure details)|
|Long Description||Data Set loaded by Wayne
There is a separate summary series for each strain and treatment combination.
These include wild type control, with peroxide and with cadmium, yap1 knockout
control and with peroxide and with cadmium, yap2 knockout control and with peroxide
and with cadmium, double knockouts control and with peroxide, wild type and fold change
numbers from wild type control for each strain and treatment.
Ohno (Ohno, S. (1970) in Evolution by Gene Duplication, (Springer,
New York)) proposed that gene duplication with subsequent divergence of
paralogs could be a major force in the evolution of new gene functions.
In practice the functional differences between closely related homologs
produced by duplications can be subtle, and difficult to separate
experimentally. Here we show that DNA microarrays can distinguish
the functions of two closely related homologs from the yeast
Saccharomyces cerevisiae, Yap1p and Yap2p. Although Yap1p and
Yap2p are both bZIP transcription factors involved in multiple stress
responses and are 88% identical in their DNA binding domains, our work
shows that these proteins have non-overlapping functions. Yap1p controls
a set of genes involved in detoxifying the effects of reactive oxygen
species, whereas Yap2p controls a set of genes over represented for the
function of stabilizing proteins. In addition we show that the binding
sites in the promoters of the Yap1p dependent genes differ from the sites
in the promoters of Yap2p dependent genes and we validate experimentally
that these differences are important for regulation by Yap1p. We conclude
that while Yap1p and Yap2p may have some overlapping functions they are
clearly not redundant and, more generally, that DNA microarray analysis
will be an important tool for distinguishing the functions of the large
numbers of highly conserved genes found in all eukaryotic genomes.
Please contact Wayne Rindone for more information, or with any questions, comments, or concerns.
Copyright (c) 2006 by Wayne Rindone and the President and Fellows of Harvard University