Format of oligo files
Both of the following tab-delimited files have the same format:
|
Column Header(s) |
Description |
|
oligo |
oligonucleotide sequence, 5' to 3' |
|
x and y |
coordinates on array |
|
affy_ID |
name given to feature by Affymetrix |
|
start and stop |
start and stop of oligo in genome |
|
dir |
orientation of oligo ("+" is the watson strand, "-" is crick) |
|
hit |
25/25 is a perfect match oligo, 24/25 is a mismatch oligo |
|
loc |
location of oligo relative to a predicted ORF or RNA (bnumber) |
|
start and stop |
start and stop of predicted ORF or RNA (bnumber) in genome |
|
dir |
orientation of predicted ORF or RNA (bnumber) in genome (">" is the watson strand, "<" is crick) |
The original array design was by D. J. Lockhart, E. M. Johansson, and M. Mittman at Affymetrix. The incorporation of oligo BLAST results against the MG1655 genome was done by Adnan Derti at the Lipper Center for Computational Genetics.