| Information Item | Value |
|---|---|
| Dataset Name | snf/swi mutant expression analysis |
| Dataset Number | 44 |
| Short Description | microarray analysis of knockout mutants of snf and swi, two components of the Snf/Swi complex |
| Source URL | http://genome-www.stanford.edu/swisnf/ |
| Reference | Sudarsanam, P; Iyer, VR; Brown, PO; and Winston, F, Whole-genome expression analysis of snf/swi mutants of Saccharomyces cerevisiae, Proc. Natl. Acad. Sci. USA 2000, 97(7):3364-3369 |
| Strains | Strains isogenic to S288C: FY2 (MATalpha ura3-52), FY31 (MATalpha ura3-52 his3delta200 snfdelta10:2000:HIS3), FY1882 (MATalpha ura3-52 leu2delta1 swidelta10:2000:LEU2) |
| Conditions | Grown to cell density of 1-2 x 10**7 cells/ml in rich medium (yeast extract/peptone/dextrose containing 2% glucose) or in synthetic minimal medium (yeast nitrogen base without amino acids plus ammonium sulfate), supplemented with uracil and 2% glucose. |
| Date Added to ExpressDB | Sep 22 2000 4:01:28:613PM |
| Number of Measures on ExpressDB | 14 (here to download dataset and view measure details) |
| Long Description | Abstract - The Saccharomyces cerevisiae Snf/Swi complex has been previously demonstrated to control transcription and chromatin structure of particular genes in vivo and to remodel nucleosomes in vitro. We have performed whole-genome expression analysis, using DNA microarrays, to study mutants deleted for a gene encoding one conserved (Snf2) or one unconserved (Swi1) Snf/Swi component. This analysis was performed on cells grown in both rich and minimal media. The microarray results, combined with Northern blot, computational, and genetic analyses, show that snf2Delta and swi1Delta mutations cause similar effects on mRNA levels, that Snf/Swi controls some genes differently in rich and minimal media, and that Snf/Swi control is exerted at the level of individual genes rather than over larger chromosomal domains. In addition, this work shows that Snf/Swi controls mRNA levels of MATalpha-specific genes, likely via controlling transcription of the regulators MATalpha1 and MCM1. Finally, we provide evidence that Snf/Swi acts both as an activator and as a repressor of transcription, and that neither mode of control is an indirect effect of the other. |
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Copyright (c) 2006 by Wayne Rindone and the President and Fellows of Harvard University