| Information Item | Value |
|---|---|
| Dataset Name | Rosetta Compendium |
| Dataset Number | 45 |
| Short Description | data series corresponding to 300 diverse mutations and chemical treatments |
| Source URL | http://www.rii.com/tech/pubs/cell_hughes.htm |
| Reference | Hughes, T.R.; et al (Full citation is in LongDescrip field); Functional Discovery via a Compendium of Expression Profiles; Cell 102:109-126(2000) |
| Strains | A detailed listing of strains used is included in the LongDescrip field |
| Conditions | NONE |
| Date Added to ExpressDB | Oct 4 2000 2:12:25:500PM |
| Number of Measures on ExpressDB | 900 (here to download dataset and view measure details) |
| Long Description | Abstract - Ascertaining the impact of uncharacterized perturbations on the cell is a fundamental problem in biology. Here, we describe how a single assay can be used to monitor hundreds of different cellular functions simultaneously. We constructed a reference database or "compendium" of expression profiles corresponding to 300 diverse mutations and chemical treatments in S. cerevisiae, and we show that the cellular pathways affected can be determined by pattern matching, even among very subtle profiles. The utility of this approach is validated by examining profiles caused by deletions of uncharacterized genes: we identify and experimentally confirm that eight previously uncharacterized open reading frames encode proteins required for sterol metabolism, cell wall function, mitochondrial respiration, or protein synthesis. We also show that the compendium can be used to characterize pharmacological perturbations by identifying a previously unknown target of the commonly used drug dyclonine. Full citation: Hughes, T.R.; Marton, M.J.; Jones, A.R.; Roberts, C.J.; Stoughton, R.; Armour, C.D.; Bennett, H.A.; Coffey, E.; Dai, Hongyue; He, Y.D.; Kidd, M.J.; King, A.M.; Meyer, M.R.; Slade, D.; Lum, Pek Y.; Stepaniants, S.B.; Shoemaker, D.D.; Gachotte, D.; Chakraburtty, K.; Simon, J.; Bard, M.; and Friend, S.H.; Functional Discovery via a Compendium of Expression Profiles; Cell 102:109-126(2000) To download a comprehensive tab-delimited list of all the strains used in these experiment, right click here and chose the Save Link As option on the menu that comes up. To download a similar tab-delimited list describing all 300 experiments, right click here and chose the Save Link As option on the menu that comes up. |
Please contact Wayne Rindone for more information, or with any questions, comments, or concerns.
Copyright (c) 2006 by Wayne Rindone and the President and Fellows of Harvard University