Expression Data Set 47 details

Information Item Value
Dataset Name Jelinsky study of RPN4 links
Dataset Number 47
Short Description Data showing coregulated DNA excision repair genes and protein degradation genes
Source URL NONE currently
Reference Jelinsky, S.; Estep, P.; Church, G.; Samson, L.;
Regulatory Networks Revealed by Transcriptional Profiling Of Damaged Saccharomyces cerevisiae Cells: RPN4 Links Base Exciession Repair with Proteasomes;
Mol Cell Biol. 20(21):8157-67; (2000)
Strains DBY747 (MATa his3-delta1 leu2-3,112, ura3-52,trp1-289a gal2
can1 CUP1s) or BY4740 and its deltarpn4 derivative (MATa ura3delta0
lys2delta0 leu2delta0 deltarpn4)
Conditions Log phase cells grown to 5x10**6 cells/ml, split, half treated with
methyl methanesulfonate (MMS) or nitrosoguanidine (MNNG) or mitomycin C (MMC) or t-BuOOH
Date Added to ExpressDB Nov 20 2000 1:33:27:373PM
Number of Measures on ExpressDB 34 (here to download dataset and view measure details)
Long Description Abstract from Web site: Exposure to carcinogenic
alkylating agents, oxidizing agents, and ionizing radiation modulates
transcript levels for over one third of Saccharomyces cerevisiae's
6,200 genes. Computational analysis delineates groups of coregulated
genes whose upstream regions bear known and novel regulatory sequence
motifs. One group of coregulated genes contains a number of DNA
excision repair genes (including the MAG1 3-methyladenine DNA
glycosylase gene) and a large selection of protein degradation
genes. Moreover, transcription of these genes is modulated by the
proteasome-associated protein Rpn4, most likely via its binding to
MAG1 upstream repressor sequence 2-like elements, that urn out to be
almost identical to the recently identified proteasome-associated
control element (G. Mannhaupt, R. Schnall, V. Karpov, I. Vetter, and
H. Feldman, FEBS Lett. 450:27-34, 1999). We have identified a large
number of genes whose transcription is influenced by Rpn4p.

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Copyright (c) 2006 by Wayne Rindone and the President and Fellows of Harvard University