| Information Item | Value |
|---|---|
| Dataset Name | Jelinsky study of RPN4 links |
| Dataset Number | 47 |
| Short Description | Data showing coregulated DNA excision repair genes and protein degradation genes |
| Source URL | NONE currently |
| Reference | Jelinsky, S.; Estep, P.; Church, G.; Samson, L.; Regulatory Networks Revealed by Transcriptional Profiling Of Damaged Saccharomyces cerevisiae Cells: RPN4 Links Base Exciession Repair with Proteasomes; Mol Cell Biol. 20(21):8157-67; (2000) |
| Strains | DBY747 (MATa his3-delta1 leu2-3,112, ura3-52,trp1-289a gal2 can1 CUP1s) or BY4740 and its deltarpn4 derivative (MATa ura3delta0 lys2delta0 leu2delta0 deltarpn4) |
| Conditions | Log phase cells grown to 5x10**6 cells/ml, split, half treated with methyl methanesulfonate (MMS) or nitrosoguanidine (MNNG) or mitomycin C (MMC) or t-BuOOH |
| Date Added to ExpressDB | Nov 20 2000 1:33:27:373PM |
| Number of Measures on ExpressDB | 34 (here to download dataset and view measure details) |
| Long Description | Abstract from Web site: Exposure to carcinogenic alkylating agents, oxidizing agents, and ionizing radiation modulates transcript levels for over one third of Saccharomyces cerevisiae's 6,200 genes. Computational analysis delineates groups of coregulated genes whose upstream regions bear known and novel regulatory sequence motifs. One group of coregulated genes contains a number of DNA excision repair genes (including the MAG1 3-methyladenine DNA glycosylase gene) and a large selection of protein degradation genes. Moreover, transcription of these genes is modulated by the proteasome-associated protein Rpn4, most likely via its binding to MAG1 upstream repressor sequence 2-like elements, that urn out to be almost identical to the recently identified proteasome-associated control element (G. Mannhaupt, R. Schnall, V. Karpov, I. Vetter, and H. Feldman, FEBS Lett. 450:27-34, 1999). We have identified a large number of genes whose transcription is influenced by Rpn4p. |
Please contact Wayne Rindone for more information, or with any questions, comments, or concerns.
Copyright (c) 2006 by Wayne Rindone and the President and Fellows of Harvard University