| Information Item | Value |
|---|---|
| Dataset Name | Genomic Expression Programs in Response to Environmental Changes |
| Dataset Number | 65 |
| Short Description | Expression Time Series following treatment with a variety of stresses, including temperature shifts, osmolarity, esposure to peroxide, menadione, diamide, DTT, amino acid starvation, and nitrogen depletion. |
| Source URL | http://www-genome.stanford.edu/yeast_stress/ |
| Reference | Gasch, AP; Spellman, PT; Kao, CM; Carmel-Harel, O; Eisen, MB; Storz, G; Botstein, D; Brown, PO; Genomic Expression Programs in the Response of Yeast Cells to Environmental Changes; Mol Biol Cell 11: 4241-4257 (2000) |
| Strains | Strain genotypes are given in Long Description. The strain names are DBY7286, DBY8768, DBY9434, DBY9435, DBY9439, DBY9440, and DBY9441 |
| Conditions | Grown on YPD at 30 C and shaken at 250-300 rpm. |
| Date Added to ExpressDB | Sep 9 2002 12:46:12:156PM |
| Number of Measures on ExpressDB | 173 (here to download dataset and view measure details) |
| Long Description | Abstract from reference: We explored genomic expression patterns in the yeast Saccharomyces cerevisiae responding to diverse environmental transitions. DNA microarrays were used to measure changes in transcript levels over time for almost every yeast gene, as cells responded to temperature shocks, hydrogen peroxide, the superoxide-generating drug menadione, the sulfhydryl-oxidizing agent diamide, the disulfide-reducing agent dithiothreitol, hyper- and hypo-osmotic shock, amino acid starvation, nitrogen source depletion, and progression into stationary phase. A large set of genes (~ 900) showed a similar drastic response to almost all of these environmental changes. Additional features of the genomic responses were specialized for specific conditions. Promoter analysis and subsequent characterization of the responses of mutant strains implicated the transcription factors Yap1p, as well as Msn2p and Msn4p, in mediating specific features of the transcriptional response, while the identification of novel sequence elements provided clues to novel regulators. Physiological themes in the genomic responses to specific environmental stresses provided insights into the effects of those stresses on the cell. |
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Copyright (c) 2006 by Wayne Rindone and the President and Fellows of Harvard University