These were the scaling factors* and background values used in GAPS© for the analyses reported in Selinger et al, Nature Biotechnology, December 2000.
Experiment Name |
Background |
Scale factor |
s of Background |
log1.CEL |
565 |
211.99 |
7.4 |
log2.CEL |
318 |
540.28 |
5.5 |
stat1.CEL |
781 |
8.26 |
27.1 |
stat2.CEL |
325 |
93.83 |
10.6 |
gDNA.CEL |
800 |
6.05 |
41.7 |
stat1_cDNA_revcomp.CEL |
899 |
20.37 |
52.6 |
log_RNA_revcomp.CEL |
535 |
7.8 |
31.4 |
*Chips were scaled to total chip intensity using Genechip© with the target intensity set to 5,000. Features for spiked controls were masked. Note that the s of the background has been provided solely for the users benefit and is not needed as input by GAPS©.