These were the scaling factors* and background values used in GAPS© for the analyses reported in Selinger et al, Nature Biotechnology, December 2000.

 

Experiment Name

Background

Scale factor

s of Background

log1.CEL

565

211.99

7.4

log2.CEL

318

540.28

5.5

stat1.CEL

781

8.26

27.1

stat2.CEL

325

93.83

10.6

gDNA.CEL

800

6.05

41.7

stat1_cDNA_revcomp.CEL

899

20.37

52.6

log_RNA_revcomp.CEL

535

7.8

31.4

*Chips were scaled to total chip intensity using Genechip© with the target intensity set to 5,000. Features for spiked controls were masked. Note that the s of the background has been provided solely for the users benefit and is not needed as input by GAPS©.