Polony Sequencing Protocols

NEWS

• 02/05/06 CORRECTION: the library protocol describes a non-standard way of doing a phenol chloroform extraction and ethanol precipitation. It is not correct. Perform a standard phenol chloroform and EtOH ppt when indicated throughout the protocol.

• 02/05/06 CORRECTION: the sequencing protocol lists some oligo sequences which are incorrect. Below are the correct ones (corrections in red):

• T30UIA CGGCCGUACGUCCAACT
• LIGFIXD2T30B /5Phos/AGUCGGAGGCCAAGG