ࡱ> 0/ \p George Church Ba==Hp8X@"1jArial1jArial1jArial1jArial1*j Comic Sans MS1*j Comic Sans MS1jArial1jArial1jArial1 jArial1$jArial1jArial1jArial1jArial1jArial1jArial1jArial"$"#,##0_);\("$"#,##0\)!"$"#,##0_);[Red]\("$"#,##0\)""$"#,##0.00_);\("$"#,##0.00\)'""$"#,##0.00_);[Red]\("$"#,##0.00\)7*2_("$"* #,##0_);_("$"* \(#,##0\);_("$"* "-"_);_(@_).))_(* #,##0_);_(* \(#,##0\);_(* "-"_);_(@_)?,:_("$"* #,##0.00_);_("$"* \(#,##0.00\);_("$"* "-"??_);_(@_)6+1_(* #,##0.00_);_(* \(#,##0.00\);_(* "-"??_);_(@_) 0.0E+00"Yes";"Yes";"No""True";"True";"False""On";"On";"Off"],[$ -2]\ #,##0.00_);[Red]\([$ -2]\ #,##0.00\)0.0 0E+0 "$"#,##0.00 "$"#,##0.000 #,##0.000 0.000 0.0E+0 0.E+00                + ) , *    "8 "x "8 "x " " " " " ! " " " !x  " " #| ! "  "  "  `~CostErr , Err-cover"bZ  3  @@  )-CoverageSBL ErrABI Err454 Err 454 $/kbABI $/kbSBL $/kb;454 error rates from Table 2 of Margulies et al Nature 20059SBL cost and error rates from Shendure et al Science 2005 SBL-estimated ABI-estimated 454-estimated SBL2 $/kbMEstimated required coverage = c1+(c1-c2)*[log(e)-log(e1)] / [log(e1)-log(e2)]SA method to compare costs at given error rates for proposed sequencing technologiesErrors8Human diploid genome goal = $1K/6e6 kb @ error<1e-8 =  $/kbUSBL2 scenario: as SBL except 4 CCD pixel per bp on each cycle instead of current 750,a plus lower fractional cost of library for human, simple savings on reagents, and dual flow cell.G(exact size of gaps as yet unspecified but expected similar to current) dThe above formula is inapplicable once the error rate plateaus into "systematic" (non-random) errors;ABI cost and error estimates from Broad Inst. & Agencourt ^454 cost estimate: 26.7 Mbp/($5K reagents+$500K/(3*365*4) 3yr_equip amortization 4 runs/day) SbL Coverage ABI Coverage" cc  %8"$  dMbP?_*+%wMHP OfficeJet R Series< d,,HP OfficeJet R Series\\GMC_SOLIS\PRINTER3 ,,d"dXX??U} } q } }  } } } q}  %w@w@w@w@w@w@w@w@w@ w@ w@ w@ w@ w@w@w@w@w@w@w@w@w@w@w@w@w@w@w@w@w@w@ $ $#'g%? `VA $ (' $$        ~ ?~jth?~ "@'&hUMu? Da2U0*3?~ V@6%MbX9? Q??(\?"F⼑? }Lh~ @v !>!Q? D@D!&'>I? D@D%~ $@ ư>!"@" D@D %~ D@ #㈵? r)?!k,@ D@D              ~ ?  #߰1!@  F <DDDLBmL@BmL@BmL@Bm "ba?   L@L MbP?   L  "q%?  F <DDDLBmL@BmL@BmL@Bm! I=H? D@D ! &D{ *? D@D   #+5@   "8@  -C6? "ȸ͒?@ p\su?  L@L!&K7?  D@DR"d{ 5@<DDDDBmD@BmD@BmD@Bm!"tomc@ D@D#ehA@ "՗@ h㈵> "dfɟ@ {`?!&NDSA? D@DR"ٞew@<DDDDBmD@BmD@BmD@Bm!"][ۘ@ D@D#/ d˧G@ "{ڮ]"@ ư> "df@ 3??!&cF? D@DR"$@<DDDDBmD@BmD@BmD@Bm!""@ D@D#bnN@ "ը+]'@ Jz> "dfɟ@ )B?!& #%#L? D@DR">M9D+@<DDDDBmD@BmD@BmD@Bm!"QR3(@ D@D#?xR@ "],@ ;0yE> "2nu!@ ܞ5đ?!& 7[P? D@DR">M9D1@<DDDDBmD@BmD@BmD@Bm!" $g/@  D@D#aU@ "0@ !           $$$$     ) ) ) )~ *? +++ba?*MbP?+N?++8@BXC& p|cj(*p5T;;;; 8. w@!w@"w@#w@$w@ *-C6? +,1Y ? + ot@ +՗@!*h㈵>!+1/ ?!+KR7@!+{ڮ]"@"*ư>"+?"+ -I<ȴ!@"+ը+]'@#*Jz>#+sK?#+\ (@#+],@$*;0yE>$+fā?$+4\}t.@$+0@PHHHH  (  p  6NMM?p']`v  "??3`  `  4(f3d23 M NM4 3Q: SBL $/kbQ ;$Q ;$Q3_4E4 3Q: ABI $/kbQ ;$Q ;$Q3_4E4 3Q: 454 $/kbQ ;$Q ;$Q3_4E4D$% Mp13O& Q4$% Mp13O& Q4FAo=b 3Oc c3*"-N#M43*N#M! M4523  O43d" C 3OD % M3OQ443_ M NM ] MM<4444eee > @7  ;.5  dMbP?_*+%MnMicrosoft Office Document Imag/d,,Letterwidm"d,,??U}  ,tttt t   , ,,,,,8 $  $  (  $   ~ ?~jth?a2U0*3?~ @v !>~ $@ư>~ D@     $ $~ ?  MbP?   L  "q%?  F <DDDLBmL@BmL@BmL@Bm -C6? "ȸ͒?@'" R"d{ 5@<DDDDBmD@BmD@BmD@Bmh㈵> "dfɟ@ R"ٞew@<DDDDBmD@BmD@BmD@Bmư> "df@ R"$@<DDDDBmD@BmD@BmD@BmJz> "dfɟ@ R">M9D+@<DDDDBmD@BmD@BmD@Bm;0yE> "2nu!@ R">M9D1@<DDDDBmD@BmD@BmD@Bm*h*2***    (  j  0NMM?OW]` v  " ??3` | ` | Xf3d23 M NM4 3Q:   SbL CoverageQ ; Q ; Q3_4E4 3Q:   ABI CoverageQ ; Q ; Q3_4E4D$% Mp13O&Q4$% Mp13O&Q4FA 3OnqP3 b#M43*#M! M4523  O43" \c3OXg6% M3O&Q423 M NM44444eee >@  7 Oh+'0HP\t George ChurchGeorge ChurchMicrosoft Excel@{y@挦՜.+,D՜.+, PXh px Harvard CostErr Err-cover  WorksheetsL@(@_AdHocReviewCycleID_EmailSubject _AuthorEmail_AuthorEmailDisplayName_ReviewingToolsShownOnce DCost per Q30 KB and Q40 KB g1m1c1@receptor.med.harvard.eduGeorge Church   !"#$%&()*+,-.Root Entry FA*Workbook=SummaryInformation(DocumentSummaryInformation8'