X   Phy Chem Bio Med Soc
Reading P1 C1 B1 M1 S1
Writing P2 Chip Libraries

GRO

DNA Chip libraries The highly parallel synthesis of oligonucleotides lept forward from 96 in 1992 in 2004 to thousands in 2004 via photo-deprotection of the 5' ends and later ink-jet and elctrolytic methods. This has enabled libraries of RNAi, CRISPR, and digital data storage.

Relevant companies: Gen9bio, Agilent Technologies.

Relevant Church Lab Publications:
2013 Causes and effects of N-terminal codon bias in bacterial genes. Science.
2013 Composability of regulatory sequences controlling transcription and translation in E. coli. PNAS.
2010 A Scalable Gene Synthesis Platform Using High-Fidelity DNA Microchips. Nature Biotech.
2004 Accurate Multiplex Gene Synthesis from Programmable DNA Chips. Nature.
1992 Parallel Sequential Reactor. Patent WO 92/21079A1.

B2 M2 S2
Arithmetic P3 C3 B3 M3 S3