What is Life?
This project is aimed at synthesis of new polymers on synthetic ribosomes, including polyketides and mirror polypeptides. As few as 150 gene products may be needed. Each of these has now been synthesized in vitro, purified and reconstituted.
See: Synthetic Biology Project (SBP), based on DARPA MiniGenome 2001. Relevant Lab Publications:
2014 Multiplex single-molecule interaction profiling of DNA barcoded proteins. Nature.
2013 In vitro integration of ribosomal RNA synthesis, ribosome assembly and translation. MSB.
2007 Synthetic biology projects in vitro. Genome Res.
2006 Toward Synthesis of a Minimal Cell. MSB.