Tm calculator using the Nearest-Neighbor method (NN paramters are based on SantaLucia J Jr. (1998) PNAS, 95:1460-5)
Tm is adjusted for MgCl2 and DMSO concentration based on von Ahsen et al. Clinical Chemistry 47: 1956-61 (2001)
Oligonucleotide Sequence:
Primer concentration (nM):
Sodium/Potasium concentration (mM):
Magnesium concentration (mM):
dNTP concentration (mM):
Percentage of DMSO (%)
Percentage of annealed temlate(%)