ORF	        5' primer	        5'Tm	3' primer	        3'Tm	len	note
YBL091C-A	CGTTACACTTCCTATCCCGGC	62.9	GTAGTATATCCAGCCGACGAGCA	62.7	 268	empty
YCL026C-A	AGCCCGAATTACCACAAGGTGTC	65.5	CTGGCAAGGCAGTTGGAACAC	65.2	 474	empty
YIR020W-B	GCCTACCGGCGTTCCTATTG	63.9	GGAATACCGGATTGTGTGCCTAC	63.8	  99	empty
YLR035C-A	TATCGAGAGTACCGGTTCGGG	63.4	GTTCTTGCACAAGGTGACTGAGG	63.4	1985	empty
YOR298C-A	GTCGCAGAAGGATTTGGCAACC	67.2	TGGAGCTCCCAAAGGCGAAC	67.0	 163	empty
YFL034C-B	CCTTAATGTTGCTCAGCCGCC	66.2	TAACGGTAGCAGCGGAGCCATT	67.0	 763	missing
YHR039C-A	ACTGTGCTCTCTTGTTGGACTGG	63.0	GGACTTCAGCAGAAGGCTTGATG	64.7	 411	missing
YJL205C-A	AGTCGATGTGCTCTTTCCCAAGC	66.1	GCGATCGTCCCATCGCTTCT	67.0	 162	missing
YLR390W-A	CTGTGTTGCGCAAGTCGGCA	69.1	CATAGCGGCAACAGCAGCAATGA	69.5	 619	missing
YOL052C-A	TTTCTGCCATCTCTGTCTTCGGC	67.1	AAGGCCAAAGCACCACCAACAG	67.6	 154	missing