JSEPCR E.coli ORF Fragments

	JSE 1,2	JSE 3,4	JSE 5,6	JSE 7,8	JSE 9,10	JSE 11,12	JSE 13,14	JSE 15,16	JSE 17,18	JSE 19,20	JSE 21,22	JSE 23,24

1	aceB_1	adi_1	glgP_2	accA_1	deoC_1	glpT_1	malX_2	pitA_1	sapB_1	acrR_1	rcsA_1	atpA_2
2	aceB_2	adi_2	glnA_1	accB_S	deoD_1	gltA_1	manX_1	pitB_1	sapD_1	ada_1	rcsB_1	atpD_1
3	acnA_1	agp_1	glnB_S	accD_1	dgkA_S	gltJ_1	manY_1	pncB_1	sbp_1	adiY_1	rhaR_1	atpE_S
4	acnA_2	alr_1	glnD_1	aceA_1	dgt_1	gltK_1	manZ_1	pnuC_1	sdaB_1	agaR_1	rhaS_1	hisD_1
5	acnA_3	ansA_1	glnD_2	aceE_1	dgt_2	gltL_1	mdh_1	potA_1	sdaC_1	alpA_S	rob_1	hisF_1
6	aldH_1	ansB_1	glnG_1	aceE_2	dld_1	gltP_1	melB_1	potB_1	sdhA_1	appY_1	rpoD_1	hybC_2
7	aldH_2	appB_1	glnL_1	aceF_1	dld_2	gltS_1	menA_1	potC_1	sdhA_2	araC_1	rpoD_2	gcvP_1
8	aroA_1	argA_1	gltA_1	aceF_2	dppA_1	gltX_1	menB_1	potD_1	sdhB_1	arsR_S	rpoE_S	gcvP_2
9	aroA_2	argB_1	gltB_1	ackA_1	dppA_2	gmk_1	menC_1	potE_1	sdhC_S	ascG_1	rpoH_1	glnP_1
10	asnA_1	argC_1	gltB_2	acnB_1	dppB_1	gnd_1	menD_1	potF_1	sdhD_S	asnC_S	rpoN_1	frdA_2
11	atpA_1	argD_1	gltB_3	acnB_2	dppC_1	gntK_S	menD_2	potG_1	serA_1	atoC_1	rpoS_1	galK_1
12	atpA_2	argE_1	gltB_4	acpS_S	dppD_1	gntT_1	menE_1	potH_1	serB_1	betI_S	sdiA_1	livG_1
13	atpB_1	argF_1	gltD_1	acs_1	dppF_1	gntV_S	menF_1	potI_1	serC_1	yjjR_1	sfsA_1	malX_2
14	atpI_S	argG_1	glyA_1	acs_2	dut_S	gor_1	menG_S	poxB_1	speA_1	birA_1	b1642_S	menD_1
15	avtA_1	argH_1	goaG_1	add_1	eda_1	gpmA_1	metA_1	poxB_2	speA_2	cbl_1	soxR_S	nadE_1
16	avtA_2	argI_1	gpmB_1	adhC_1	entA_1	gpsA_1	metF_1	ppa_S	speB_1	celD_1	soxS_S	nhaB_2
17	fba_1	argR_S	hisA_1	adhE_1	entB_1	gpt_S	metK_1	proA_1	speC_1	criR_1	tdcA_1	pstA_1
18	fba_2	aroA_1	hisB_1	adhE_2	entC_1	gshA_1	mglA_1	proP_1	speC_2	crp_1	tdcR_S	pstB_1
19	b1517_1	aroB_1	hisC_1	adk_1	entD_1	gshA_2	mglA_2	proP_2	speD_1	cynR_1	treR_1	pstC_1
20	b1773_1	aroC_1	hisD_1	agaY_1	entE_1	gshB_1	mglB_1	proV_1	speE_1	cysB_1	trpR_S	pstS_1
21	b2097_1	aroD_1	hisF_1	agaZ_1	entE_2	gsk_1	mglC_1	proW_1	speF_1	cytR_1	ttk_1	pta_1
22	b2097_2	aroE_1	hisG_1	aldA_1	entF_1	guaA_1	mraY_1	proX_1	speF_2	deoR_1	tyrR_1	pta_2
23	cspE_S	aroF_1	hisH_S	aldB_1	entF_2	guaA_2	msbB_1	psd_1	srlA_1_S	dicA_S	tyrR_2	ptsA_1
24	cydB_1	aroG_1	hisI_S	aldB_2	entF_3	guaB_1	mtlA_1	pssA_1	srlA_2_1	dicC_S	uhpA_S	ptsA_2
25	cydB_2	aroH_1	hyaB_1	amn_1	epd_1	guaC_1	mtlA_2	pstA_1	srlB_S	dsdC_1	uvrY_1	ptsG_1
26	cyoA_1	aroK_1	hyaB_2	amtB_1	fabD_1	hemA_1	mtlD_1	pstB_1	srlD_1	ebgR_1	uxuR_1	ptsI_1
27	cysD_1	aroL_S	hybC_1	appC_1	fabH_1	hemB_1	mtr_1	pstC_1	sucA_1	emrR_S	xapR_1	ptsI_2
28	edd_1	aroM_1	hybC_2	appC_2	fadB_1	hemC_1	murA_1	pstS_1	sucA_2	envR_1	xylR_1	ptsN_S
29	edd_2	asd_1	hycF_S	apt_S	fadB_2	hemD_1	murB_1	pta_1	sucC_1	envY_1	b0294_S	ptsP_1
30	eno_1	asnA_1	ilvA_1	araA_1	fadL_1	hemE_1	murC_1	pta_2	talB_1	evgA_S	yafC_1	ptsP_2
31	eno_2	asnB_1	ilvA_2	araA_2	fba_1	hemF_1	murD_1	ptsA_1	tdcB_1	exuR_1	yahA_1	purA_1
32	frdB_1	asnB_2	ilvB_1	araB_1	fbp_1	hemH_1	murE_1	ptsA_2	tdcC_1	fadR_1	ybeF_1	purB_1
33	fumC_1	asnC_S	ilvB_2	araB_2	fdnH_1	hemK_1	murF_1	ptsG_1	tdh_1	farR_1	ybhD_1	purC_1
34	fumC_2	aspA_1	ilvC_1	araD_1	fdoG_1	hemL_1	murG_1	ptsI_1	tdk_S	fecI_S	ybiH_1	purD_1
35	gapC_1_S	aspC_1	ilvD_1	araE_1	fdoG_2	hemM_1	murI_1	ptsI_2	thiC_1	fhlA_1	ydhB_1	purE_S
36	gdhA_1	atpA_1	ilvD_2	araF_1	fdoG_3	hemX_1	mutT_S	ptsN_S	thiC_2	fhlA_2	yehV_1	purF_1
37	gdhA_2	atpA_2	ilvE_1	araG_1	focA_1	hemY_1	nadA_1	ptsP_1	thiE_1	fimZ_1	yeiE_1	purF_2
38	glk_1	atpC_S	ilvG_1_1	araG_2	folA_S	hisF_1	nadB_1	ptsP_2	thiF_1	fis_S	yfeG_1	purH_1
39	glnA_1	atpD_1	ilvG_2_1	araH_S	folC_1	hisJ_1	nadB_2	purA_1	thiG_1	fliA_1	yfhA_1	purH_2
40	glnA_2	atpE_S	ilvH_S	argT_1	folD_1	hisM_1	nadC_1	purB_1	thiH_1	fnr_1	yfiE_1	purK_1
41	gltA_1	atpF_S	ilvI_1	aroA_1	folE_1	hisP_1	nadE_1	purC_1	thrA_1	fruR_1	ygaA_1	purL_3
42	gltA_2	atpH_S	ilvI_2	aroP_1	folK_S	hisQ_1	nagA_1	purD_1	thrA_2	frvR_1	ygaA_2	pyrG_1
43	hisF_1	avtA_1	ilvM_S	artI_1	folP_1	hpt_S	nagB_1	purE_S	thrB_1	frvR_2	b2738_1	pyrI_S
44	icdA_1	cadA_1	ilvN_S	artJ_1	frdA_1	htrB_1	nagE_1	purF_1	thrC_1	fucR_1	b2869_1	thrB_1
45	icdA_2	cadA_2	ilvY_1	artM_1	frdA_2	hyaA_1	nagE_2	purF_2	thyA_1	fur_S	b2869_2	trxB_1
46	ilvA_1	carA_1	kbl_1	artP_1	fruA_1	hyaC_1	nanA_1	purH_1	tmk_1	galR_1	b3060_1	acrR_1
47	ilvA_2	carB_1	ldcC_1	artQ_1	fruA_2	hybA_1	nanT_1	purH_2	tnaA_1	galS_1	yhaJ_1	ada_1
48	leuA_1	carB_2	ldcC_2	asnA_1	fruB_1	hycB_S	nanT_2	purK_1	tnaB_1	gcvA_1	b3226_1	adiY_1
49	leuA_2	carB_3	leuA_1	atoB_1	fruK_1	hycE_1	ndh_1	purL_1	treA_1	glcC_1	b3243_1	fhlA_2
50	lpp_S	cbl_1	leuA_2	atpA_1	fucA_1	hycE_2	ndk_S	purL_2	treA_2	glpG_1	yhdM_S	gusR_S
51	lysA_1	cydC_1	leuB_1	atpA_2	fucI_1	hycG_1	nhaA_1	purL_3	treB_1	glpR_1	yhfR_1	gutM_S
52	lysA_2	cydC_2	leuC_1	atpG_1	fucI_2	ilvA_1	nhaB_1	purM_1	trkA_1	gntR_1	yhgB_1	srlR_1
53	metA_1	cyoA_1	leuD_S	avtA_1	fucK_1	ilvA_2	nhaB_2	purN_1	trkG_1	gusR_S	yhgB_2	hipB_S
54	nuoA_S	cyoB_1	leuO_1	bglX_1	fucO_1	ispA_1	nrdA_1	purT_1	trkH_1	gutM_S	yhiE_S	b2491_2
55	pckA_1	cyoB_2	lysA_1	bglX_2	fucP_1	ispB_1	nrdA_2	purU_1	trpB_1	srlR_1	yhiF_S	pssR_S
56	pckA_2	cyoC_S	lysC_1	bioA_1	fumA_1	kdpA_1	nrdB_1	putA_1	trpC_1	hipB_S	b3515_1	b0294_S
57	pflB_1	cyoD_S	lysR_1	bioB_1	fumA_2	kdpA_2	nrdD_1	putA_2	trpD_1	hydG_1	yhiX_1	b2869_1
58	pflB_2	cysB_1	malP_1	bioD_1	fumB_1	kdpB_1	nrdD_2	putA_3	trpD_2	b2491_1	yhjB_S	b3243_1
59	pflB_3	cysC_S	malP_2	bioF_1	fumB_2	kdpB_2	nrdE_1	putP_1	trpE_1	b2491_2	yhjC_1	yhgB_1
60	pgi_1	cysE_1	metA_1	brnQ_1	gabP_1	kdpC_S	nrdE_2	putP_2	trpE_2	iciA_1	yiaJ_1	yhiX_1
61	pgi_2	cysH_1	metB_1	cadB_1	galE_1	kdsA_1	nrdF_1	pykA_1	trxB_1	iclR_1	yiaU_1	yjbK_S
62	pgk_1	cysI_1	metC_1	cdd_1	galF_1	kdsB_1	ntpA_S	pykF_1	tsx_1	ilvY_1	b3680_1	yjdA_1
63	pgk_2	cysI_2	metE_1	cdh_1	galK_1	kdtA_1	nuoA_S	pyrB_1	tyrB_1	lacI_1	yidP_1	yjhI_1
64	pheA_1	cysJ_1	metE_2	cdsA_1	galP_1	kgtP_1	nupC_1	pyrC_1	tyrP_1	leuO_1	yidZ_1	cspE_S
65	pheA_2	cysJ_2	metH_1	chaA_1	galT_1	lacY_1	nupG_1	pyrD_1	ubiA_1	lexA_S	b3872_1	glk_1
66	pntA_1	cysK_1	metH_2	chaB_S	galU_1	lacZ_1	oppA_1	pyrE_1	ubiB_1	lctR_1	yihW_1	ilvM_S
67	pntA_2	cysM_1	metH_3	chaC_1	gapA_1	lacZ_2	oppA_2	pyrF_1	ubiC_S	lrhA_1	yijO_1	ilvN_S
68	ppc_1	cysN_1	metJ_S	cls_1	gapC_2_S	lacZ_3	oppB_1	pyrG_1	ubiG_1	lrp_S	yjbK_S	leuO_1
69	ppc_2	dadA_1	metL_1	cmk_1	gatA_S	lamB_1	oppC_1	pyrG_2	ubiH_1	lysR_1	yjdA_1	yhaD_1
70	ppc_3	dadX_1	metL_2	cmtA_1	gatB_S	lctD_1	oppD_1	pyrH_1	ubiX_S	malI_1	yjdA_2	yhbU_1
71	ppsA_1	dapA_1	metR_1	cmtB_S	gatC_1	ldhA_1	oppF_1	pyrI_S	udk_1	malT_1	yjfQ_1	yhiN_1
72	ppsA_2	dapB_1	nuoA_S	coaA_1	gatD_1	leuA_1	pabA_S	rbsA_1	udp_1	malT_2	yjgS_1	yieG_1
73	ppsA_3	dapD_1	nuoB_1	codA_1	gatY_1	leuA_2	pabB_1	rbsA_2	ugpA_1	b1384_1	yjhI_1	yihZ_S
74	proA_1	dapE_1	nuoE_S	codB_1	gcvH_S	lig_1	pabC_1	rbsB_1	ugpB_1	marA_S	yjhJ_1	yjgF_S
75	proA_2	dapF_1	nuoF_1	crr_S	gcvP_1	lig_2	panB_1	rbsC_1	ugpC_1	marR_S	yjhU_1	yaeC_1
76	rpiA_1	dsdA_1	nuoG_1	cycA_1	gcvP_2	livF_1	panC_1	rbsD_S	ugpE_1	melR_1	yjiE_1	ybeB_S
77	rpiB_S	fba_1	nuoG_2	cydA_1	gcvT_1	livG_1	panD_S	rbsK_1	upp_1	metR_1	yjiR_1	ycfH_1
78	sdaA_1	fdhF_1	nuoH_1	cydA_2	gdhA_1	livH_1	panF_1	rfaC_1	uraA_1	b0346_1	yjjQ_1	b1435_1
79	sdaA_2	fdhF_2	nuoI_S	cydD_1	glcB_1	livJ_1	pdxA_1	rfaD_1	ushA_1	modE_1	cspE_S	b1435_2
80	sfcA_1	fdnG_1	nuoJ_S	cydD_2	glcB_2	livK_1	pdxB_1	rfaF_1	ushA_2	nac_1	glk_1	ydiB_1
81	sfcA_2	fdnG_2	nuoK_S	cyoA_1	glmS_1	livM_1	pdxH_1	rfaG_1	xapA_1	narL_1	icdA_2	yebI_1
82	sucB_1	fdnG_3	nuoL_1	cyoE_1	glmS_2	lldP_1	pdxJ_1	rfaI_1	xapB_1	narP_1	ilvA_2	yeeC_1
83	sucB_2	fdnI_1	nuoL_2	cysA_1	glmU_1	lldP_2	pfkA_1	rfaJ_1	xylA_1	nhaR_1	gltB_3	b2081_1
84	sucD_1	fdoH_1	nuoM_1	cysG_1	glnA_1	lpdA_1	pfkB_1	rfaL_1	xylB_1	nlp_S	hisI_S	yfcB_1
85	tktA_1	fdoI_1	nuoM_2	cysP_1	glnH_1	lpxA_1	pflA_1	rhaA_1	xylE_1	oxyR_1	hyaB_1	yfgB_1
86	tktA_2	frdC_S	nuoN_1	cysU_1	glnP_1	lpxB_1	pflB_1	rhaB_1	xylF_1	pdhR_1	ilvH_S	yfhC_S
87	tktB_1	frdD_S	pflB_1	cysW_S	glnQ_1	lpxC_1	pflB_2	rhaD_1	xylG_1	perR_1	ilvI_1	ygcA_1
88	tktB_2	gabD_1	pflB_2	cysZ_1	glpA_1	lpxD_1	pflC_1	rhaT_1	xylG_2	phnF_1	ilvM_S	ygfA_S
89	tktB_3	gabT_1	pheA_1	dcd_S	glpA_2	lysA_1	pflD_1	ribA_S	xylH_1	phnO_S	ilvN_S	b2954_S
90	tpiA_1	gadA_1	pntB_1	dctA_1	glpB_1	lysP_1	pflD_2	ribB_1	yaaC_1	pin_S	leuO_1	b2955_1
91	trpA_1	gadB_1	proA_1	dcuA_1	glpC_1	malE_1	pgm_1	ribD_1	ygiG_S	b0330_1	metA_1	yjhG_1
92	tynA_1	galM_1	proB_1	dcuB_1	glpD_1	malF_1	pgm_2	ribE_1	ygjG_1	b0330_2	metB_1	yjhG_2
93	tynA_2	gdhA_1	proC_1	ddlA_1	glpD_2	malF_2	pgpB_1	ribH_S	ygjH_S	pspF_1	metC_1	yjiR_1
94	tynA_3	glgA_1	prsA_1	ddlB_1	glpF_1	malG_1	pgsA_S	rpe_1	yicP_1	pssR_S	metR_1	yohI_1
95	tyrA_1	glgC_1	putA_1	deoA_1	glpK_1	malK_1	pheA_1	sapA_1	yicP_2	purR_1	proA_1	b2385_1
96	tyrA_2	glgP_1	putA_2	deoB_1	glpK_2	malX_1	pheP_1	sapA_2	zwf_1	rbsR_1	fdoG_3	yfiH_1

Oligo (& PCR) Placement by PS960 on 96 well plate

	1	2	3	4	5	6	7	8	9	10	11	12
A	89	81	73	65	57	49	41	33	25	17	9	1
B	90	82	74	66	58	50	42	34	26	18	10	2
C	91	83	75	67	59	51	43	35	27	19	11	3
D	92	84	76	68	60	52	44	36	28	20	12	4
E	93	85	77	69	61	53	45	37	29	21	13	5
F	94	86	78	70	62	54	46	38	30	22	14	6
G	95	87	79	71	63	55	47	39	31	23	15	7
H	96	88	80	72	64	56	48	40	32	24	16	8

For example: On set JSE 1,2, aceB_1 is in spot A12, tyrA_2 is in spot H1.

Back to Lam

	1	2	3	4	5	6	7	8	9	10	11	12
A	89	81	73	65	57	49	41	33	25	17	9	1
B	90	82	74	66	58	50	42	34	26	18	10	2
C	91	83	75	67	59	51	43	35	27	19	11	3
D	92	84	76	68	60	52	44	36	28	20	12	4
E	93	85	77	69	61	53	45	37	29	21	13	5
F	94	86	78	70	62	54	46	38	30	22	14	6
G	95	87	79	71	63	55	47	39	31	23	15	7
H	96	88	80	72	64	56	48	40	32	24	16	8

	1	2	3	4	5	6	7	8	9	10	11	12
A	89	81	73	65	57	49	41	33	25	17	9	1
B	90	82	74	66	58	50	42	34	26	18	10	2
C	91	83	75	67	59	51	43	35	27	19	11	3
D	92	84	76	68	60	52	44	36	28	20	12	4
E	93	85	77	69	61	53	45	37	29	21	13	5
F	94	86	78	70	62	54	46	38	30	22	14	6
G	95	87	79	71	63	55	47	39	31	23	15	7
H	96	88	80	72	64	56	48	40	32	24	16	8

	1	2	3	4	5	6	7	8	9	10	11	12
A	89	81	73	65	57	49	41	33	25	17	9	1
B	90	82	74	66	58	50	42	34	26	18	10	2
C	91	83	75	67	59	51	43	35	27	19	11	3
D	92	84	76	68	60	52	44	36	28	20	12	4
E	93	85	77	69	61	53	45	37	29	21	13	5
F	94	86	78	70	62	54	46	38	30	22	14	6
G	95	87	79	71	63	55	47	39	31	23	15	7
H	96	88	80	72	64	56	48	40	32	24	16	8