Description of column headings for Tables a1 to
A prefix "M" is from E. coli cells harvested during
growth phase in glucose-minimal media and "R" is from
cells harvested at early stationary phase in rich media.
N-terminal Sequence Tag
The N-terminal amino acid sequence tag. A single upper case letter indicates an unambiguous PTHaa signal in the cycle. A lower case letter indicates that the PTHaa signal is close to the level of background noise or lag. Parentheses indicate that a cycle had two or more PTHaa signals. The order of amino acids in parentheses is from strongest to weakest PTHaa signals. An upper case "X" indicates no PTHaa signal could be detected above the background lag. A lower case "x" indicates instrument failure in
the cycle and no amino acid call. The symbol "XXXXXXXXXXXX"
indicates that no PTHaa sequence signal was detected above the
background lag during the entire sequencing run.
Sequence Tag Match to Genomic ORF
The observed protein sequence compared to the amino terminal sequence
of the matching conceptual protein in the E. coli genome. The
predicted sequence is shown and the observed sequence is in bold.
An "*" in the protein sequence shows the observed start
site based on the N-terminal sequence tag. An underlined amino
acid indicates a discrepancy between the predicted sequence and
the observed sequence tag. The predicted amino acid is shown.
For query-sequence matching the internal region of an E. coli
gene, the numbers of amino acids between the predicted N- and
C- termini of the conceptual protein that match the start and
stop sites of the sequence tag are shown in parentheses.
Locus - The E. coli gene symbol assigned to the ORF identified
by the protein sequence. The symbol "NM" indicates that
the query string was used to search the database, but no significant
match was found.
cpI - The predicted isoelectric point calculated from the
conceptual sequence of the protein.
opI - The observed isoelectric point of the protein calculated
from the 2-DE gel (x) coordinate of the spot.
MW - The predicted mass calculated from the conceptual
sequence of the protein. The units are kDa.
Mr - The observed mass of the protein calculated from the
2-DE gel (y) coordinate of the spot. The units are kDa.
O-abd - The observed abundance on the master 2-DE gel.
N-abd - The estimated cellular abundance of the protein
species. The units are molecules/cell.