Description of column headings for Tables a1 to a10

Spot ID

A prefix "M" is from E. coli cells harvested during growth phase in glucose-minimal media and "R" is from cells harvested at early stationary phase in rich media.

N-terminal Sequence Tag

The N-terminal amino acid sequence tag. A single upper case letter indicates an unambiguous PTH_aa signal in the cycle. A lower case letter indicates that the PTH_aa signal is close to the level of background noise or lag. Parentheses indicate that a cycle had two or more PTH_aa signals. The order of amino acids in parentheses is from strongest to weakest PTH_aa signals. An upper case "X" indicates no PTH_aa signal could be detected above the background lag. A lower case "x" indicates instrument failure in the cycle and no amino acid call. The symbol "XXXXXXXXXXXX" indicates that no PTH_aa sequence signal was detected above the background lag during the entire sequencing run.

Sequence Tag Match to Genomic ORF

The observed protein sequence compared to the amino terminal sequence of the matching conceptual protein in the E. coli genome. The predicted sequence is shown and the observed sequence is in bold. An "*" in the protein sequence shows the observed start site based on the N-terminal sequence tag. An underlined amino acid indicates a discrepancy between the predicted sequence and the observed sequence tag. The predicted amino acid is shown. For query-sequence matching the internal region of an E. coli gene, the numbers of amino acids between the predicted N- and C- termini of the conceptual protein that match the start and stop sites of the sequence tag are shown in parentheses.

Locus - The E. coli gene symbol assigned to the ORF identified by the protein sequence. The symbol "NM" indicates that the query string was used to search the database, but no significant match was found.

cpI^-The predicted isoelectric point calculated from the conceptual sequence of the protein.

opI^-The observed isoelectric point of the protein calculated from the 2-DE gel (x) coordinate of the spot.

MW - The predicted mass calculated from the conceptual sequence of the protein. The units are kDa.

Mr - The observed mass of the protein calculated from the 2-DE gel (y) coordinate of the spot. The units are kDa.

O-abd^-The observed abundance on the master 2-DE gel.

N-abd - The estimated cellular abundance of the protein species. The units are molecules/cell.

Table a1. Growth-phase, minimal-media E. coli proteins from a SDS/heat extract

Predicted versus Observed sequence, pI, MW Abundance

------------------------------------------------------------------------------- -------------------------

Spot O- N-

I.D. N-terminal Sequence Tag^b Sequence Tag Match to Genomic ORF^c Locus^d cpI^e opI^f MW^g Mr^h abdⁱ abd^j

M1 SVIKMT m*svikmt pgk 4.93 5.27 41.0 39.8 0.56 2990

M2-1 (MSD)(QDL)(IG)(NSK)(VT)(ARKT) m*slintk ahpC 4.90 5.16 20.6 39.7 0.32 2250

M2-2 (MSD)(QDL)(IG)(NSK)(VT)(ARKT) mqgsvt rpoA 4.81 5.16 36.5 39.7 0.12 850

M3 ATGKIT m*atgkiv atpD 4.74 5.03 50.2 47.8 0.32 1210

M4 MQVSVE mqvsve tig 4.64 4.98 48.2 51.7 0.33 1070

M5 TDKLT(AS) m*tdklts talB 4.94 5.18 35.1 34.9 0.29 380

M6 AEITAX m*aeitas tsf 5.07 5.33 30.3 33.0 0.49 2670

M7 MRIILLGAPGAG mriillgapgag adk 5.53 5.81 23.6 26.4 0.56 1640

M8 APKDNTXYTGAK mkktaiaiavalagfatvaqa*apkdntwytg
ak
ompA 5.74 5.85 35.2 33.1 0.31 2300

M9 (Mq)KVAVLGAAGGI mkvavlgaagg mdh 5.56 5.94 32.3 34.1 0.42 2640

M10-1 (ST)(KI)(IA)(FX)(EV)(Di)(Ng)(ST)(LX)(Tg)(IX)
(Ga)
m*skifednsltig cysK 5.94 6.17 34.4 33.8 0.44 2820

M10-2 (ST)(KI)(IA)(FX)(EV)(Di)(Ng)(ST)(LX)(Tg)(IX)
(Ga)
m*tiaivigthgwa manX 5.87 6.17 34.9 33.8 0.03 220

M11 ANPLYQKHIISI m*anplyqkhiis pyrB 6.75 6.56 34.3 33.5 0.34 2080

M12 SILIDKNTKVIX m*silidkntkvic sucD 6.77 6.68 29.6 30.0 0.42 900

M13 AVTKLVLVRHGE m*avtklvlvrhge gpmA 6.11 6.21 28.4 26.7 0.45 960

M14 AAKDVKFGNDAR m*aakdvkfgndar mopA 4.67 5.02 57.2 57.7 0.33 2180

M15 SKIVKIIGR m*skivkiigr eno 5.23 5.60 45.5 44.4 0.35 2160

M16 (Mqh)LKRE(Mh)NIADYD mlkremniadyd glyA 6.46 6.41 45.3 43.8 0.45 3180

M17 (Mqth)(Ka)(It)VEVKHPLVK mkivevkhplvk upp 5.23 5.47 22.5 22.9 0.49 2580

M18-1 (ETMA)(DQS)(IX)(KF)(VNH)(AF)(VS)(VX)(GX)
(AP)(Mh)SG
mntkgkallaglialafsnmala*edikvavvg
amsg
livJ 5.17 5.44 36.8 39.4 0.51 10140

M18-2 (ETMA)(DQS)(IX)(KF)(VNH)(AF)(VS)(VX)(GX)(AP)(Mh)SG NM 5.44 39.4 0.04 820

M19 (Mq)LRIAKEALTFD mlriakealtfd guaB 6.38 6.41 52.0 53.5 0.16 600

M20 ADVPAGVTLAEK mtnitkrslvaagvlaalmagnvala*advpag
vtlaek
oppA 6.17 6.31 58.4 55.1 0.21 760

M21 GLFDKLKSLVSD m*glfdklkslvsd crr 4.56 4.81 18.1 18.5 0.66 3540

M22 MNXEILAVVEXV mnkeilavveav nusA 4.35 4.70 54.9 63.7 0.08 340

M23 TIKVGIDGFGRI m*tikvgingfgri gapA 7.11 7.31 35.4 35.4 0.60 1840

M24 (Mqk)RLEFSIYRYN mrlefsiyryn sdhB 6.68 6.66 26.8 25.6 0.36 480

M25 AVGLHYFSK (36)k*avglhyfsk(313) ompF 4.43 4.70 35.3 34.0 0.24 1980

M26 AP(KV)D(NK)(Td)m(YV)Vsr(kv) NM 4.97 27.1 0.53 480

M27 (Sk)(AG)EHVLTMLN m*saehvltmln glnA 5.23 5.38 51.8 54.5 0.15 420

M28 SAEHVLTMLNE m*saehvltmlne glnA 5.23 5.44 51.8 54.1 0.14 1040

M29-1 (MSq)(NDM)(Prt)(Lk)(KI)(AD)(GV)(Dks)(IX)(AL)
(Pg)(Kr)
mnplkagdiapk bcp 4.93 5.15 17.6 15.0 0.54 920

M29-2 (MSq)(NDM)(Prt)(Lk)(KI)(AD)(GV)(Dks)(IX)(AL)
(Pg)(Kr)
NM 5.15 15.0 0.06 100

M30-1 (AP)(DPL)(YL)(DL)(DSI)(dfs)(kft)(etv)(vd) M*PLLDSFTVD ygaG 5.13 5.26 19.3 19.5 0.26 260

M30-2 (AP)(DPL)(YL)(DL)(DSI)(dfs)(kft)(etv)(vd) MPLLDSFTV ygaG 5.13 5.26 19.4 19.5 0.18 180

M31-1 SL(IL)N(TV)(PK)(IA)(GK)(KP)(DF)(LK)(NP) m*slintkikpfkn ahpC 4.90 5.15 20.6 21.6 0.68 6040

M31-2 SL(IL)N(TV)(PK)(IA)(GK)(KP)(DF)(LK)(NP) m*sllnvpagkdlp ppa 4.90 5.15 19.6 21.6 0.62 5480

M32 SQTVHFQGNPV(TS) M*SQTVHFQGNPVT tpx 4.59 5.02 17.8 17.4 0.68 2480

M33-1 (MAQHS)(EK)(SHQ)(KLQ)(VIF)(VGT)(VS)(PRE)
(AS)(QHM)(GAS)(KV)
meskvvvpaqgk icdA 5.00 5.25 45.8 44.8 0.56 8180

M33-2 (MAQHS)(EK)(SHQ)(KLQ)(VIF)(VGT)(VS)(PRE)
(AS)(QHM)(GAS)(KV)
NM 5.25 44.8 0.20 2900

M34 TIKVGINGFG m*tikvgingfg gapA 7.11 6.77 35.4 35.5 0.64 740

M35 MRXPLVMGNXKL mrhplvmgnwkl tpiA 5.89 5.85 27.0 25.4 0.23 1120

M36 SFELPALPYAKD m*sfelpalpyakd sodB 5.88 5.80 21.1 20.7 0.56 2020

M37 AEKINFGVSATY mkklvlaallasftfgasa*aekinfgvsaty artJ 6.29 6.32 24.9 24.1 0.48 1180

M38-1 (SM)(YI)(TS)(LD)(PI)(sr)(LK)(PD)(Ya)(AE)(YV)
(DR)
m*sytlpslpyayd sodA 6.98 6.96 23.0 21.9 1.45 2980

M38-2 (SM)(YI)(TS)(LD)(PI)(sr)(LK)(PD)(Ya)(AE)(YV)
(DR)
misdirkdaevr frr 6.86 6.96 20.6 21.9 0.40 840

M39 AKIKGQVKXFNE m*akikgqvkwfne cspC 7.53 7.45 7.3 16.0 0.61 8300

M40 MVTFHTNHGDIV mvtfhtnhgdiv ppiB 5.64 5.80 18.2 16.1 0.53 780

M41 MQVILLDKVANL mqvilldkvanl rplI 6.56 6.68 15.8 14.0 1.05 3400

M42-1 (MT)(TH)(HD)(DN)(Nk)(KL)(LQ)(QV)(VE)(EA)
(AI)(Ik)
mthdnklqveai pyrI 7.37 7.61 17.1 16.0 0.55 760

M42-2 (MT)(TH)(HD)(DN)(Nk)(KL)(LQ)(QV)(VE)(EA)
(AI)(Ik)
m*thdnklqveaik pyrI 7.37 7.61 17.0 16.0 0.32 440

M43 GKIIGIDLGTTN m*gkiigidlgttn dnaK 4.67 4.97 69.0 68.3 0.37 1300

M44-1 (MX)(TE)(ES)(SF)(FA)(AQ)(QL)(LF)(FE) mtesfaqlf rpsA 4.71 5.03 61.2 66.5 0.23 820

M44-2 (MX)(TE)(ES)(SF)(FA)(AQ)(QL)(LF)(FE) m*tesfaqlfe rpsA 4.71 5.03 61.0 66.5 0.15 540

M45 MISGILAXPGI misgilaspgi ptsI 4.60 4.91 63.6 61.1 0.08 360

M46 AAKDVKFGNDAR m*aakdvkfgnda mopA 4.67 4.97 57.2 58.7 0.09 360

M47 DDNNTLYFYNXT mkkwsrhllaagalalgmsaaha*ddnntlyf
ynwt
potD 4.69 4.92 36.5 35.1 0.29 1040

M48 TQFAFVFPGQ m*tqfafvfpgq fabD 4.79 5.08 32.3 32.4 0.19 500

M49 (Mq)FEPMELTNDAV mfepmeltndav ftsZ 4.47 4.76 40.3 39.3 0.12 320

M50 AE(Vi)YNKDGN mkvkvlsllvpallvagaana*aevynkdgn ompC 4.33 4.54 38.3 36.7 0.50 6020

M51 AEI(Yi)NKDGNKVD mmkrnilavivpallvagtana*aeiynkdgn
kvd
ompF 4.48 4.61 37.1 36.7 0.25 2160

M52-1 (AX)(IP)(PK)(QD)(NX)(IT)(RX)(IY)(GX)(TX)
(DA)(PK)
MKKLVLSLSLVLAFSSATAALA*A
IPQNIRIGTDP
hisJ 5.01 5.24 26.2 27.5 0.35 3400

M52-2 (AX)(IP)(PK)(QD)(NX)(IT)(RX)(IY)(GX)(TX)
(DA)(PK)
mkktaiaiavalagfatvaqa*apkdntwytg
ak
ompA 5.74 5.24 35.2 27.5 0.34 3380

M53-1 (DA)(EL)(GP)(LE)(LT)(NV)(KR)(VI)(KG)(EX)
(RD)(GX)
MKLAHLGRQALMGVMAVALVAG
MSVKSFA*DEGLLNKVKERG
fliY 5.14 5.27 26.1 25.3 0.45 1920

M53-2 (DA)(EL)(GP)(LE)(LT)(NV)(KR)(VI)(KG)(EX)
(RD)(GX)
mkksilalsllvglstaassya*alpetvrigtdt argT 5.05 5.27 25.8 25.3 0.21 900

M54 MQKQAELYRG(Kr)A mqkqaelyrgka purC 4.88 5.18 27.0 25.8 0.39 1680

M55 ARTTPIARYRNI m*arttpiaryrni fusA 5.13 5.47 77.4 81.4 0.06 1080

M56 (Ma)(Lr)EEYRK(Hr)VA(EG) VLEEYRKHVAE yacI 5.14 5.43 93.5 88.4 0.02 120

M57 (SM)EKTFLVEIGTe m*sektflveigte glyS 5.18 5.47 76.7 70.8 0.03 80

M58 x(Qi)(Ev)s(Lp)RNIA(Vi)IA mieklrniaiia yihK 4.97 5.40 65.4 73.1 0.01 60

M59 MLNPIVRKFQYG mlnpivrkfqyg pnp 4.96 5.28 77.1 76.2 0.03 200

M60 AEGFVVKDIXFE mamkklliasllfssatvyg*aegfvvkdihfe yaeT 4.72 4.98 88.4 81.8 0.01 180

M61 AEGFVVKDIHF(Ev) mamkklliasllfssatvyg*aegfvvkdihfe yaeT 4.72 4.96 88.4 82.6 0.01 80

M62 ADLAS MKKRIPTLLATMIATALYSQQGL
A*ADLAS
imp 4.70 4.92 87.1 83.0 0.01 60

M63-1 (Ma)(Nd)(Li)(Hfk)(Ev)(Yf)(QX)(Av)KQLF mnlheyqakqlf sucC 5.28 5.53 41.4 39.7 0.08 340

M63-2 (Ma)(Nd)(Li)(Hfk)(Ev)(Yf)(QX)(Av)KQLF NM 5.53 39.7 0.02 80

M64 (Mq)KNVGFIGGRGM mknvgfigwrgm asd 5.32 5.54 40.0 38.0 0.14 900

M65 A(QD)IFNFSSGPAM m*aqifnfssgpam serC 5.33 5.59 39.7 39.3 0.27 2500

M66-1 (SM)(KFq)(IE)(FN)(DI)(FT)(VGa)(KA)(Pi)(GA)
(VD)(IPSy)
m*skifdfvkpgvi fba 5.75 5.83 39.0 39.4 0.14 1480

M66-2 (SM)(KFq)(IE)(FN)(DI)(FT)(VGa)(KA)(Pi)(GA)
(VD)(IPSy)
mfenitaapadp aspC 5.58 5.83 43.6 39.4 0.08 460

M67-1 (Mast)(NI)(YK)(QV)(NG)(DI)(DX)(Lg)(Rf)(IX)(KX)(Ea) mnyqnddlrike aroG 6.58 6.55 38.0 35.7 0.22 600

M67-2 (Mast)(NI)(YK)(QV)(NG)(DI)(DX)(Lg)(Rf)(IX)
(KX)(Ea)
M*TIKVGINGFGRI gapA 7.11 6.55 35.4 35.7 0.08 220

M68-1 (Mas)(NL)(LD)(TK)(EI)(LV)(KI)(NA)(TN)(PR)
(GV)(Esil)
mnltelkntpvs rho 7.29 7.52 47.0 50.8 0.08 300

M68-2 (Mas)(NL)(LD)(TK)(EI)(LV)(KI)(NA)(TN)(PR)(GV)(Esil) mldkivianrge accC 7.09 7.52 49.3 50.8 0.05 180

M69 MRIEEDLKLg(Fv)l mrieedlklgfk guaC 6.51 6.61 37.4 36.4 0.10 160

M70 (MAi)(Ed)IL(av)(dk)e(en)X(ap)s(va) NM 6.72 39.2 0.10 100

M71 ANYFNTLNLRQQ m*anyfntlnlrqq ilvC 5.07 5.45 53.9 51.2 0.14 1420

M72 ANYFNTLNLRQQ m*anyfntlnlrqq ilvC 5.07 5.38 53.9 51.7 0.08 480

M73 MKLYNLKD(HD)NE(QE) mklynlkdhneq thrC 5.16 5.50 47.1 47.0 0.11 560

M74 TTILKHLPVGqR m*ttilkhlpvgqr argG 5.14 5.41 49.8 47.4 0.17 380

M75-1 (Sma)(KNE)(IN)V(Vk)(Vi)(il)(fg)(ta)(eaqgd)ii m*skivkiigreii eno 5.23 5.40 45.5 44.6 0.11 200

M75-2 (Sma)(KNE)(IN)V(Vk)(Vi)(il)(fg)(ta)(eaqgd)ii NM 5.40 44.6 0.17 300

M76-1 (Sgam)(KN)(IN)(Vtge)(KX)(IX)(Il)(Gd)(Rtd)(Eq)
(IX)(Ivl)
m*skivkiigreii eno 5.23 5.51 45.5 44.4 0.14 1660

M76-2 (Sgam)(KN)(IN)(Vtge)(KX)(IX)(Il)(Gd)(Rtd)(Eq)
(IX)(Ivl)
M*GNNVVVLGTQWG purA 5.22 5.51 47.2 44.4 0.08 940

M77-1 (AS)(NE)(LY)(FS)(VQ)(TG)(LV)(NGP)(LQ)(rX)
(lX)(rX)
M*ANYFNTLNLRQQ ilvC 5.07 5.44 53.9 49.9 0.04 160

M77-2 (AS)(NE)(LY)(FS)(VQ)(TG)(LV)(NGP)(LQ)(rX)(lX)(rX) NM 5.44 49.9 0.02 80

M78 MEXKVVVPAQGK meskvvvpaqgk icdA 5.00 5.19 45.8 46.5 0.08 240

M79 MESKVVVPAQGK meskvvvpaqgk icdA 5.00 5.17 45.8 44.8 0.32 1480

M80-1 (ASX)(FX)(KF)(KX)(gX)(Ig)(VI)(GV)(LG)(PL)
(NP)(VN)
m*gfkcgivglpnv ychF 4.70 5.07 39.5 43.2 0.05 120

M80-2 (ASX)(FX)(KF)(KX)(gX)(Ig)(VI)(GV)(LG)(PL)
(NP)(VN)
mgfkcgivglpn ychF 4.70 5.07 39.7 43.2 0.06 140

M81 AKTLYEKLFDA(AH) m*aktlyeklfdah leuC 6.28 6.32 49.8 50.3 0.12 280

M82 ADVPAGVtLAEK mtnitkrslvaagvlaalmagnvala*advpag
vtlaek
oppA 6.17 6.16 58.4 55.1 0.05 380

M83 MKKTKIVaTIg(PG) mkktkivctigp pykF 5.98 6.12 50.7 56.0 0.07 500

M84 STEIKTQVVVL(IG) mm*steiktqvvvlg lpdA 6.10 6.15 50.6 49.9 0.07 380

M85 (Kms)(Ts)LVYXSEGsP mrislkksgmlklglslvamtvaasvqa*ktlvy
csegsp
dppA 5.94 6.05 57.4 51.4 0.13 660

M86 STEIKTQVVVlg mm*steiktqvvvlg lpdA 6.10 6.08 50.6 50.1 0.04 160

M87 MQLNSTEISELI mqlnsteiseli atpA 6.01 6.15 55.2 51.6 0.12 820

M88 (Si)NKPFHYQAPF m*snkpfhyqapf fumA 6.52 6.47 60.2 57.5 0.03 160

M89 MIKSALLVLEDG miksallvledg carA 6.35 6.22 41.4 41.8 0.18 620

M90 (MS)(Kg)LPIYLDYsAT MMYGVYRA*MKLPIYLDYSAT yhfO 6.34 6.30 45.1 41.5 0.09 180

M91-1 (AT)(IT)(EL)(LQ)(NTV)(AP)(YI)(FS)(RG)(AE)
(FT)(GF)
M*TTLLNPYFGEFG trpB 6.04 6.07 42.9 41.5 0.15 360

M91-2 (AT)(IT)(EL)(LQ)(NTV)(AP)(YI)(FS)(RG)(AE)
(FT)(GF)
M*AIEQTAITRATF argD 6.20 6.07 43.6 41.5 0.08 200

M92-1 (AM)(KL)(VX)(RS)(Le)(ME)(NK)(DI)(AK)(Id)(KY)(FX) m*akvslekdkikf serA 6.33 6.26 44.0 44.1 0.15 380

M92-2 (AM)(KL)(VX)(RS)(Le)(ME)(NK)(DI)(AK)(Id)(KY)(FX) mlkremniady glyA 6.46 6.26 45.3 44.1 0.11 300

M93 XXXXXXXXXXXX 6.30 42.5 0.05

M94 MDQTYsL(Ei)XFLN mdqtyslesfln gdhA 6.37 6.33 48.6 43.4 0.09 140

M95 TAPSQVLKIRRP m*tapsqvlkirrp pyrC 6.15 6.09 38.7 37.4 0.11 340

M96 (PA)PDM(KL)LFAGNAT mpdmklfagnat prsA 5.13 5.40 34.2 33.1 0.15 160

M97-1 (Sm)(LA)(IL)(NE)(VT)(PK)(AIL)(GK)(KP)(DF)
(LKd)(PNl)
m*sllnvpagkdlp ppa 4.90 5.04 19.6 21.9 0.24 500

M97-2 (Sm)(LA)(IL)(NE)(VT)(PK)(AIL)(GK)(KP)(DF)
(LKd)(PNl)
m*slintkikpfkn ahpC 4.90 5.04 20.6 21.9 0.14 300

M98 MD(It)ISVALKR(hp)s mdiisvalkrhs nfnB 6.17 6.17 23.9 24.1 0.22 260

M99 SKIFEDNSLTIi m*skifednslti cysK 5.94 6.01 34.4 33.7 0.12 240

M100 SLNFLDFEQPIA m*slnfldfeqpia accA 5.88 6.04 35.1 32.5 0.14 180

M101-1 (At)(Ni)(LN)(KL)(AK)(VA)(IV)(PI)(VP)(AV)(GA)
(LG)
maaint*kvkkavipvagl galU 5.00 6.06 32.3 31.4 0.08 120

M101-2 (At)(Ni)(LN)(KL)(AK)(VA)(IV)(PI)(VP)(AV)(GA)
(LG)
maain*tkvkkavipvag galU 5.00 6.06 32.4 31.4 0.09 140

M102-1 (GM)(FG)(LF)(SL)(GS)(KG)(RK)(IR)(LI)(VL)
(TL)(GT)
mgflsgkrilvt fabI 5.72 5.97 27.9 30.3 0.08 200

M102-2 (GM)(FG)(LF)(SL)(GS)(KG)(RK)(IR)(LI)(VL)
(TL)(GT)
m*gflsgkrilvtg fabI 5.72 5.97 27.7 30.3 0.09 240

M103 (VA)TYPLP(Te)DGsrL MNMKLKTLFAAAFAVVGFCSTAS
A*VTYPLPTDGSRL
ybiS 5.74 5.86 30.9 29.4 0.13 160

M104 MQQLQNIIETAF mqqlqniietaf dapD 5.53 5.85 29.9 30.3 0.13 420

M105 (As)VVASLKPVGFI MKCYNITLLIFITIIGRIMLHKKTLL
FAALSAALWGGATQAADA*AVVASLK
PVGFI
yebL 5.58 5.80 31.2 30.8 0.08 240

M106 (MSD)D(Ig)KV(At)V(Vf)GAMs mkrnaktiiagmialaishtama*ddikvavvg
ams
livK 4.94 5.07 37.0 39.8 0.13 240

M107-1 (SM)(KE)(NI)(YK)(VH)(IT)(AD)(VP)(LA)(PQ)
(GX)(DK)
M*SKNYHIAVLPGD leuB 5.03 5.26 39.4 42.4 0.12 400

M107-2 (SM)(KE)(NI)(YK)(VH)(IT)(AD)(VP)(LA)(PQ)
(GX)(DK)
NM 5.26 42.4 0.08 260

M108 TIKVGINGFGRI m*tikvgingfgri gapA 7.11 7.42 35.4 35.7 0.35 1660

M109-1 (Akst)(AI)I(NE)(TG)K(VL)K(Ik)AV(IN) m*aaintkvkkavi galU 5.00 5.34 32.8 38.3 0.07 260

M109-2 (Akst)(AI)I(NE)(TG)K(VL)K(Ik)AV(IN) NM 5.34 38.3 0.04 140

M110-1 (MX)(LM)(KL)(XK)(FX)(RF)(GR)(MG)(FM)(SF)
(LS)(DL)
mlkkfrgmfsnd mreB 5.03 5.39 37.0 37.1 0.04 60

M110-2 (MX)(LM)(KL)(XK)(FX)(RF)(GR)(MG)(FM)(SF)
(LS)(DL)
mlkkfrgmfsn mreB 5.03 5.39 37.0 37.1 0.04 80

M111 (Mra)KVAVLGAAGGI mkvavlgaaggi mdh 5.56 5.69 32.3 34.4 0.20 260

M112 MTLQqqIIKALg mtlqqqiikalg nadE 5.33 5.70 30.6 35.5 0.11 100

M113 (SAQ)GFYXKXFLKgl m*sgfyhkhflkl argI 5.51 5.70 36.8 36.9 0.09 140

M114 (AQ)Xk(Td)PLYEVXt(tl) M*aqqtplyeqhtl gcvT 5.35 5.69 40.1 38.1 0.09 80

M115 M(NI)PS(IV)I(NVD)(LY)taip mers*mkpsvilykalp yiaE 6.86 5.76 40.1 36.4 0.13 160

M116 (Ap)ENK(TD)LXIYNv mtalnkkwlsglvagalmavsvgtla*aeqktl
hiynw
potF 5.35 5.82 38.2 35.7 0.10 200

M117 ARIIVVTSGKgg m*ariivvtsgkgg minD 5.08 5.44 29.5 28.6 0.11 300

M118-1 (AM)(QP)(TX)(PD)(ND)(IT)(LX)(IY)(VY)(EG)DE mqtphilivede arcA 5.08 5.40 27.3 27.7 0.10 200

M118-2 (AM)(QP)(TX)(PD)(ND)(IT)(LX)(IY)(VY)(EG)DE MKKTAIAIAVALAGFATVAQA*A
PKDNTWYTGAK
ompA 5.74 5.40 35.2 27.7 0.07 140

M119 AVAANKRSVMTL m*avaankrsvmtl sspA 5.10 5.42 24.2 25.3 0.19 480

M120 AETIRFATEA(GS)Y mkkvliaaliagfslsata*aetirfateasy artI 5.20 5.42 25.0 24.6 0.30 340

M121 (SAmgd)ERYESLFA(Qg)LK meryeslfaqlk trpA 5.23 5.51 28.7 26.4 0.15 280

M122 A(GVL)(VI)PVAD(PV)(LV)QXR m*svvpvadvlq asnS 5.04 5.33 52.4 47.8 0.11 80

M123 ENLMQVYQ(Qg)A(Rg)L mkkllpiliglslsgfsslsqa*enlmqvyqqarl tolC 5.09 5.29 51.5 50.3 0.08 120

M124-1 M(LIV)(QKPV)X(RF)(EK)(EQ)(MG)(VA)R(VA)(IQ) NM 5.26 52.5 0.04 120

M124-2 M(LIV)(QKPV)X(RF)(EK)(EQ)(MG)(VA)R(VA)(IQ) NM 5.26 52.5 0.02 60

M125 (MS)VPVQ(Kg)P(Mr)YX(Dvd) M*SVPVQHPMYIDG aldA 4.91 5.19 52.1 52.3 0.08 280

M126 SFNTII(Dg)PnX(PYEK)t m*sfntiidwnsct hisD 5.05 5.15 46.0 49.9 0.06 220

M127 SQNVYQFIDLQR m*sqnvyqfidlqr gltD 5.52 5.81 51.9 50.3 0.08 620

M128-1 (Sq)(QS)(VN)(VD)(YI)(LGq)(VF)(IP)DL(QP)(RE) M*SSVDILVPDLPE sucB 5.62 5.70 43.9 50.3 0.06 420

M128-2 (Sq)(QS)(VN)(VD)(YI)(LGq)(VF)(IP)DL(QP)(RE) M*SQNVYQFIDLQR gltD 5.52 5.70 51.9 50.3 0.03 180

M129 SQQ(VLR)(GI)I(FGE)D(TG)Tl(Er) m*sqqviifdttlr leuA 5.56 5.71 57.1 52.5 0.05 160

M130 M(QG)Q(Gd)RPVL(dr)ALL mqqrrpvrrall purH 5.60 5.86 57.3 56.6 0.06 160

M131 iGTE(gy)XPq(qgi)XXg NM 5.71 62.9 0.04 80

M132 (SA)(DE)FVYTMXRV(LI)(Ga) m*aqfvytmhrvgk yjjK 5.24 5.67 64.9 59.4 0.03 140

M133 sEL(Lr)NXsID(lv)X(lv) mavnllkknslalvaslllaghvqa*tellnssyd
vsr
cysP 6.99 6.85 35.1 31.7 0.18 60

M134 (AM)(GM)QK(VG)VSI(gp)D(di)N mkqkvvsigdin kdsA 6.78 6.90 30.8 30.4 0.24 200

M135 MVTFsT(NI)pXXI mvtfhtnhgdi ppiB 5.64 5.53 18.2 16.0 0.09 80

M136-1 (As)(Ea)(Ke)(Rk)(Nr)(In)(Fi)(Lf)(Vl)(Gv)(Pg)(Mp) mrfqfmscraslseaglsltnslsstekm*aekrn
iflvgpm
aroK 5.11 5.51 19.4 16.6 0.16 140

M136-2 (As)(Ea)(Ke)(Rk)(Nr)(In)(Fi)(Lf)(Vl)(Gv)(Pg)(Mp) mrfqfmscraslseaglsltnslsstekm*aekrn
iflvgp
aroK 5.11 5.51 19.4 16.6 0.22 200

M137 (Sm)Q(Ei)N(QT)NRK(PS)FXL mqegqnrktssl dksA 4.89 5.15 17.5 17.2 0.44 260

M138 TTPTFDT(Ig)EAQ(ALgr) m*ttptfdtieaqa fklB 4.68 4.95 22.1 23.7 0.25 340

M139 SEALKILNNIRT m*sealkilnnirt hns 5.25 5.70 15.4 14.1 0.74 820

M140 SKTIATENAPAA M*SKTIATENAPAA yjgF 5.25 5.44 13.5 14.5 0.70 1320

M141 (AMS)RHYEIVFMVXP mrhyeivfmvhp rpsF 5.17 5.45 15.2 14.2 0.59 560

M142 MRHYEIVFMVHP mrhyeivfmvhp rpsF 5.17 5.38 15.2 14.2 0.60 1120

M143 MRHYEIVFMYHP mrhyeivfmvhp rpsF 5.17 5.25 15.2 14.2 0.67 940

M144 MNIRPLHDRVIV mnirplhdrviv mopB 4.99 5.28 10.4 14.0 0.26 980

M145 AYKHILIAVDLS m*aykhiliavdls uspA 5.02 5.22 15.9 14.1 0.75 1660

M146-1 (ASM)(ND)(TV)(IK)(DKS)(MV)(TR)I(FNr)X(AV) NM 4.69 14.6 0.24 200

M146-2 (ASM)(ND)(TV)(IK)(DKS)(MV)(TR)I(FNr)X(AV) NM 4.69 14.6 0.33 260

M147-1 (AM)(ET)(SVLN)(FNq)(TQ)(TLV)(TV)(NL)(RK)
(PRY)(FRE)D
M*AESFTTTNRYFD yifE 10.85 6.54 16.2 14.1 0.52 340

M147-2 (AM)(ET)(SVLN)(FNq)(TQ)(TLV)(TV)(NL)(RK)
(PRY)(FRE)D
NM 6.54 14.1 0.14 100

M148 ATYYSNDFRAGL m*atyysndfragl efp 4.73 5.19 20.5 23.2 0.47 1600

M149 (AS)(Drh)NNTLY(Fi)YNX(It) mkkwsrhllaagalalgmsaaha*ddnntlyf
ynwt
potD 4.69 4.84 36.5 35.3 0.11 260

M150 (Ms)IIVTGGAGFIG miivtggagfig rfaD 4.64 4.97 34.9 36.2 0.09 220

M151 APKDNTRYTGAK mkktaiaiavalagfatvaqa*apkdntwytg
ak
ompA 5.74 5.13 35.2 27.6 0.48 1480

M152 XXXXXXXXXXXX tufA/B 5.30 5.68 43.2 42.0 1.83

M153 XXXXXXXXXXXX tufA/B 5.30 5.62 43.2 41.8 1.84

M154 AEIYNKDGNKVD mmkrnilavivpallvagtana*aeiynkdgn
kvd
ompF 4.48 4.71 37.1 36.4 1.35 17600

^b) The observed N-terminal amino acid sequence of the spot. A single upper case letter indicates an unambiguous PTH_aa signal in the cycle. A lower case letter indicates that the PTH_aa signal is close to the level of background noise or lag. Parentheses indicate that a cycle had two or more PTH_aa signals. The order of amino acids in parentheses is from strongest to weakest PTH_aa signals. An upper case "X" indicates no PTH_aa signal could be detected above the background lag. A lower case "x" indicates instrument failure in the cycle and no amino acid call. The symbol "XXXXXXXXXXXX" indicates that no PTH_aa sequence signal was detected above the background lag during the entire sequencing run.

^c) The observed protein sequence compared to the amino terminal sequence of the matching conceptual protein in the E. coli genome. The predicted sequence is shown and the observed sequence is in bold. An "*" in the protein sequence shows the observed start site based on the N-terminal sequence tag. An underlined amino acid indicates a discrepancy between the predicted sequence and the observed sequence tag. The predicted amino acid is shown. For query-sequence matching the internal region of an E. coli gene, the numbers of amino acids between the predicted N- and C- termini of the conceptual protein that match the start and stop sites of the sequence tag are shown in parentheses.

^d) The E. coli gene symbol assigned to the ORF identified by the protein sequence. The symbol "NM" indicates that the query string was used to search the database, but no significant match was found. The table is sorted alphabetically by loci names.

^e) The predicted isoelectric point calculated from the conceptual sequence of the protein.

^f) The observed isoelectric point of the protein calculated from the (x) coordinates of the spot.

^g) The predicted mass calculated from the conceptual sequence of the protein. The units are kDa.

^h) The observed mass of the protein calculated from the (y) coordinates of the spot. The units are kDa.

ⁱ⁾ The observed abundance on the master 2-DE gel.

^j) The estimated cellular abundance of the protein species. The units are molecules/cell.

		Predicted versus Observed sequence, pI, MW						Abundance
		-------------------------------------------------------------------------------						-------------------------
Spot								O-	N-
I.D.	N-terminal Sequence Tag^b	Sequence Tag Match to Genomic ORF^c	Locus^d	cpI^e	opI^f	MW^g	Mr^h	abdⁱ	abd^j
M1	SVIKMT	m*svikmt	pgk	4.93	5.27	41.0	39.8	0.56	2990
M2-1	(MSD)(QDL)(IG)(NSK)(VT)(ARKT)	m*slintk	ahpC	4.90	5.16	20.6	39.7	0.32	2250
M2-2	(MSD)(QDL)(IG)(NSK)(VT)(ARKT)	mqgsvt	rpoA	4.81	5.16	36.5	39.7	0.12	850
M3	ATGKIT	m*atgkiv	atpD	4.74	5.03	50.2	47.8	0.32	1210
M4	MQVSVE	mqvsve	tig	4.64	4.98	48.2	51.7	0.33	1070
M5	TDKLT(AS)	m*tdklts	talB	4.94	5.18	35.1	34.9	0.29	380
M6	AEITAX	m*aeitas	tsf	5.07	5.33	30.3	33.0	0.49	2670
M7	MRIILLGAPGAG	mriillgapgag	adk	5.53	5.81	23.6	26.4	0.56	1640
M8	APKDNTXYTGAK	mkktaiaiavalagfatvaqa*apkdntwytg ak	ompA	5.74	5.85	35.2	33.1	0.31	2300
M9	(Mq)KVAVLGAAGGI	mkvavlgaagg	mdh	5.56	5.94	32.3	34.1	0.42	2640
M10-1	(ST)(KI)(IA)(FX)(EV)(Di)(Ng)(ST)(LX)(Tg)(IX) (Ga)	m*skifednsltig	cysK	5.94	6.17	34.4	33.8	0.44	2820
M10-2	(ST)(KI)(IA)(FX)(EV)(Di)(Ng)(ST)(LX)(Tg)(IX) (Ga)	m*tiaivigthgwa	manX	5.87	6.17	34.9	33.8	0.03	220
M11	ANPLYQKHIISI	m*anplyqkhiis	pyrB	6.75	6.56	34.3	33.5	0.34	2080
M12	SILIDKNTKVIX	m*silidkntkvic	sucD	6.77	6.68	29.6	30.0	0.42	900
M13	AVTKLVLVRHGE	m*avtklvlvrhge	gpmA	6.11	6.21	28.4	26.7	0.45	960
M14	AAKDVKFGNDAR	m*aakdvkfgndar	mopA	4.67	5.02	57.2	57.7	0.33	2180
M15	SKIVKIIGR	m*skivkiigr	eno	5.23	5.60	45.5	44.4	0.35	2160
M16	(Mqh)LKRE(Mh)NIADYD	mlkremniadyd	glyA	6.46	6.41	45.3	43.8	0.45	3180
M17	(Mqth)(Ka)(It)VEVKHPLVK	mkivevkhplvk	upp	5.23	5.47	22.5	22.9	0.49	2580
M18-1	(ETMA)(DQS)(IX)(KF)(VNH)(AF)(VS)(VX)(GX) (AP)(Mh)SG	mntkgkallaglialafsnmala*edikvavvg amsg	livJ	5.17	5.44	36.8	39.4	0.51	10140
M18-2	(ETMA)(DQS)(IX)(KF)(VNH)(AF)(VS)(VX)(GX)(AP)(Mh)SG		NM		5.44		39.4	0.04	820
M19	(Mq)LRIAKEALTFD	mlriakealtfd	guaB	6.38	6.41	52.0	53.5	0.16	600
M20	ADVPAGVTLAEK	mtnitkrslvaagvlaalmagnvala*advpag vtlaek	oppA	6.17	6.31	58.4	55.1	0.21	760
M21	GLFDKLKSLVSD	m*glfdklkslvsd	crr	4.56	4.81	18.1	18.5	0.66	3540
M22	MNXEILAVVEXV	mnkeilavveav	nusA	4.35	4.70	54.9	63.7	0.08	340
M23	TIKVGIDGFGRI	m*tikvgingfgri	gapA	7.11	7.31	35.4	35.4	0.60	1840
M24	(Mqk)RLEFSIYRYN	mrlefsiyryn	sdhB	6.68	6.66	26.8	25.6	0.36	480
M25	AVGLHYFSK	(36)k*avglhyfsk(313)	ompF	4.43	4.70	35.3	34.0	0.24	1980
M26	AP(KV)D(NK)(Td)m(YV)Vsr(kv)		NM		4.97		27.1	0.53	480
M27	(Sk)(AG)EHVLTMLN	m*saehvltmln	glnA	5.23	5.38	51.8	54.5	0.15	420
M28	SAEHVLTMLNE	m*saehvltmlne	glnA	5.23	5.44	51.8	54.1	0.14	1040
M29-1	(MSq)(NDM)(Prt)(Lk)(KI)(AD)(GV)(Dks)(IX)(AL) (Pg)(Kr)	mnplkagdiapk	bcp	4.93	5.15	17.6	15.0	0.54	920
M29-2	(MSq)(NDM)(Prt)(Lk)(KI)(AD)(GV)(Dks)(IX)(AL) (Pg)(Kr)		NM		5.15		15.0	0.06	100
M30-1	(AP)(DPL)(YL)(DL)(DSI)(dfs)(kft)(etv)(vd)	M*PLLDSFTVD	ygaG	5.13	5.26	19.3	19.5	0.26	260
M30-2	(AP)(DPL)(YL)(DL)(DSI)(dfs)(kft)(etv)(vd)	MPLLDSFTV	ygaG	5.13	5.26	19.4	19.5	0.18	180
M31-1	SL(IL)N(TV)(PK)(IA)(GK)(KP)(DF)(LK)(NP)	m*slintkikpfkn	ahpC	4.90	5.15	20.6	21.6	0.68	6040
M31-2	SL(IL)N(TV)(PK)(IA)(GK)(KP)(DF)(LK)(NP)	m*sllnvpagkdlp	ppa	4.90	5.15	19.6	21.6	0.62	5480
M32	SQTVHFQGNPV(TS)	M*SQTVHFQGNPVT	tpx	4.59	5.02	17.8	17.4	0.68	2480
M33-1	(MAQHS)(EK)(SHQ)(KLQ)(VIF)(VGT)(VS)(PRE) (AS)(QHM)(GAS)(KV)	meskvvvpaqgk	icdA	5.00	5.25	45.8	44.8	0.56	8180
M33-2	(MAQHS)(EK)(SHQ)(KLQ)(VIF)(VGT)(VS)(PRE) (AS)(QHM)(GAS)(KV)		NM		5.25		44.8	0.20	2900
M34	TIKVGINGFG	m*tikvgingfg	gapA	7.11	6.77	35.4	35.5	0.64	740
M35	MRXPLVMGNXKL	mrhplvmgnwkl	tpiA	5.89	5.85	27.0	25.4	0.23	1120
M36	SFELPALPYAKD	m*sfelpalpyakd	sodB	5.88	5.80	21.1	20.7	0.56	2020
M37	AEKINFGVSATY	mkklvlaallasftfgasa*aekinfgvsaty	artJ	6.29	6.32	24.9	24.1	0.48	1180
M38-1	(SM)(YI)(TS)(LD)(PI)(sr)(LK)(PD)(Ya)(AE)(YV) (DR)	m*sytlpslpyayd	sodA	6.98	6.96	23.0	21.9	1.45	2980
M38-2	(SM)(YI)(TS)(LD)(PI)(sr)(LK)(PD)(Ya)(AE)(YV) (DR)	misdirkdaevr	frr	6.86	6.96	20.6	21.9	0.40	840
M39	AKIKGQVKXFNE	m*akikgqvkwfne	cspC	7.53	7.45	7.3	16.0	0.61	8300
M40	MVTFHTNHGDIV	mvtfhtnhgdiv	ppiB	5.64	5.80	18.2	16.1	0.53	780
M41	MQVILLDKVANL	mqvilldkvanl	rplI	6.56	6.68	15.8	14.0	1.05	3400
M42-1	(MT)(TH)(HD)(DN)(Nk)(KL)(LQ)(QV)(VE)(EA) (AI)(Ik)	mthdnklqveai	pyrI	7.37	7.61	17.1	16.0	0.55	760
M42-2	(MT)(TH)(HD)(DN)(Nk)(KL)(LQ)(QV)(VE)(EA) (AI)(Ik)	m*thdnklqveaik	pyrI	7.37	7.61	17.0	16.0	0.32	440
M43	GKIIGIDLGTTN	m*gkiigidlgttn	dnaK	4.67	4.97	69.0	68.3	0.37	1300
M44-1	(MX)(TE)(ES)(SF)(FA)(AQ)(QL)(LF)(FE)	mtesfaqlf	rpsA	4.71	5.03	61.2	66.5	0.23	820
M44-2	(MX)(TE)(ES)(SF)(FA)(AQ)(QL)(LF)(FE)	m*tesfaqlfe	rpsA	4.71	5.03	61.0	66.5	0.15	540
M45	MISGILAXPGI	misgilaspgi	ptsI	4.60	4.91	63.6	61.1	0.08	360
M46	AAKDVKFGNDAR	m*aakdvkfgnda	mopA	4.67	4.97	57.2	58.7	0.09	360
M47	DDNNTLYFYNXT	mkkwsrhllaagalalgmsaaha*ddnntlyf ynwt	potD	4.69	4.92	36.5	35.1	0.29	1040
M48	TQFAFVFPGQ	m*tqfafvfpgq	fabD	4.79	5.08	32.3	32.4	0.19	500
M49	(Mq)FEPMELTNDAV	mfepmeltndav	ftsZ	4.47	4.76	40.3	39.3	0.12	320
M50	AE(Vi)YNKDGN	mkvkvlsllvpallvagaana*aevynkdgn	ompC	4.33	4.54	38.3	36.7	0.50	6020
M51	AEI(Yi)NKDGNKVD	mmkrnilavivpallvagtana*aeiynkdgn kvd	ompF	4.48	4.61	37.1	36.7	0.25	2160
M52-1	(AX)(IP)(PK)(QD)(NX)(IT)(RX)(IY)(GX)(TX) (DA)(PK)	MKKLVLSLSLVLAFSSATAALAA IPQNIRIGTDP*	hisJ	5.01	5.24	26.2	27.5	0.35	3400
M52-2	(AX)(IP)(PK)(QD)(NX)(IT)(RX)(IY)(GX)(TX) (DA)(PK)	mkktaiaiavalagfatvaqa*apkdntwytg ak	ompA	5.74	5.24	35.2	27.5	0.34	3380
M53-1	(DA)(EL)(GP)(LE)(LT)(NV)(KR)(VI)(KG)(EX) (RD)(GX)	MKLAHLGRQALMGVMAVALVAG MSVKSFA*DEGLLNKVKERG	fliY	5.14	5.27	26.1	25.3	0.45	1920
M53-2	(DA)(EL)(GP)(LE)(LT)(NV)(KR)(VI)(KG)(EX) (RD)(GX)	mkksilalsllvglstaassya*alpetvrigtdt	argT	5.05	5.27	25.8	25.3	0.21	900
M54	MQKQAELYRG(Kr)A	mqkqaelyrgka	purC	4.88	5.18	27.0	25.8	0.39	1680
M55	ARTTPIARYRNI	m*arttpiaryrni	fusA	5.13	5.47	77.4	81.4	0.06	1080
M56	(Ma)(Lr)EEYRK(Hr)VA(EG)	VLEEYRKHVAE	yacI	5.14	5.43	93.5	88.4	0.02	120
M57	(SM)EKTFLVEIGTe	m*sektflveigte	glyS	5.18	5.47	76.7	70.8	0.03	80
M58	x(Qi)(Ev)s(Lp)RNIA(Vi)IA	mieklrniaiia	yihK	4.97	5.40	65.4	73.1	0.01	60
M59	MLNPIVRKFQYG	mlnpivrkfqyg	pnp	4.96	5.28	77.1	76.2	0.03	200
M60	AEGFVVKDIXFE	mamkklliasllfssatvyg*aegfvvkdihfe	yaeT	4.72	4.98	88.4	81.8	0.01	180
M61	AEGFVVKDIHF(Ev)	mamkklliasllfssatvyg*aegfvvkdihfe	yaeT	4.72	4.96	88.4	82.6	0.01	80
M62	ADLAS	MKKRIPTLLATMIATALYSQQGL A*ADLAS	imp	4.70	4.92	87.1	83.0	0.01	60
M63-1	(Ma)(Nd)(Li)(Hfk)(Ev)(Yf)(QX)(Av)KQLF	mnlheyqakqlf	sucC	5.28	5.53	41.4	39.7	0.08	340
M63-2	(Ma)(Nd)(Li)(Hfk)(Ev)(Yf)(QX)(Av)KQLF		NM		5.53		39.7	0.02	80
M64	(Mq)KNVGFIGGRGM	mknvgfigwrgm	asd	5.32	5.54	40.0	38.0	0.14	900
M65	A(QD)IFNFSSGPAM	m*aqifnfssgpam	serC	5.33	5.59	39.7	39.3	0.27	2500
M66-1	(SM)(KFq)(IE)(FN)(DI)(FT)(VGa)(KA)(Pi)(GA) (VD)(IPSy)	m*skifdfvkpgvi	fba	5.75	5.83	39.0	39.4	0.14	1480
M66-2	(SM)(KFq)(IE)(FN)(DI)(FT)(VGa)(KA)(Pi)(GA) (VD)(IPSy)	mfenitaapadp	aspC	5.58	5.83	43.6	39.4	0.08	460
M67-1	(Mast)(NI)(YK)(QV)(NG)(DI)(DX)(Lg)(Rf)(IX)(KX)(Ea)	mnyqnddlrike	aroG	6.58	6.55	38.0	35.7	0.22	600
M67-2	(Mast)(NI)(YK)(QV)(NG)(DI)(DX)(Lg)(Rf)(IX) (KX)(Ea)	M*TIKVGINGFGRI	gapA	7.11	6.55	35.4	35.7	0.08	220
M68-1	(Mas)(NL)(LD)(TK)(EI)(LV)(KI)(NA)(TN)(PR) (GV)(Esil)	mnltelkntpvs	rho	7.29	7.52	47.0	50.8	0.08	300
M68-2	(Mas)(NL)(LD)(TK)(EI)(LV)(KI)(NA)(TN)(PR)(GV)(Esil)	mldkivianrge	accC	7.09	7.52	49.3	50.8	0.05	180
M69	MRIEEDLKLg(Fv)l	mrieedlklgfk	guaC	6.51	6.61	37.4	36.4	0.10	160
M70	(MAi)(Ed)IL(av)(dk)e(en)X(ap)s(va)		NM		6.72		39.2	0.10	100
M71	ANYFNTLNLRQQ	m*anyfntlnlrqq	ilvC	5.07	5.45	53.9	51.2	0.14	1420
M72	ANYFNTLNLRQQ	m*anyfntlnlrqq	ilvC	5.07	5.38	53.9	51.7	0.08	480
M73	MKLYNLKD(HD)NE(QE)	mklynlkdhneq	thrC	5.16	5.50	47.1	47.0	0.11	560
M74	TTILKHLPVGqR	m*ttilkhlpvgqr	argG	5.14	5.41	49.8	47.4	0.17	380
M75-1	(Sma)(KNE)(IN)V(Vk)(Vi)(il)(fg)(ta)(eaqgd)ii	m*skivkiigreii	eno	5.23	5.40	45.5	44.6	0.11	200
M75-2	(Sma)(KNE)(IN)V(Vk)(Vi)(il)(fg)(ta)(eaqgd)ii		NM		5.40		44.6	0.17	300
M76-1	(Sgam)(KN)(IN)(Vtge)(KX)(IX)(Il)(Gd)(Rtd)(Eq) (IX)(Ivl)	m*skivkiigreii	eno	5.23	5.51	45.5	44.4	0.14	1660
M76-2	(Sgam)(KN)(IN)(Vtge)(KX)(IX)(Il)(Gd)(Rtd)(Eq) (IX)(Ivl)	M*GNNVVVLGTQWG	purA	5.22	5.51	47.2	44.4	0.08	940
M77-1	(AS)(NE)(LY)(FS)(VQ)(TG)(LV)(NGP)(LQ)(rX) (lX)(rX)	M*ANYFNTLNLRQQ	ilvC	5.07	5.44	53.9	49.9	0.04	160
M77-2	(AS)(NE)(LY)(FS)(VQ)(TG)(LV)(NGP)(LQ)(rX)(lX)(rX)		NM		5.44		49.9	0.02	80
M78	MEXKVVVPAQGK	meskvvvpaqgk	icdA	5.00	5.19	45.8	46.5	0.08	240
M79	MESKVVVPAQGK	meskvvvpaqgk	icdA	5.00	5.17	45.8	44.8	0.32	1480
M80-1	(ASX)(FX)(KF)(KX)(gX)(Ig)(VI)(GV)(LG)(PL) (NP)(VN)	m*gfkcgivglpnv	ychF	4.70	5.07	39.5	43.2	0.05	120
M80-2	(ASX)(FX)(KF)(KX)(gX)(Ig)(VI)(GV)(LG)(PL) (NP)(VN)	mgfkcgivglpn	ychF	4.70	5.07	39.7	43.2	0.06	140
M81	AKTLYEKLFDA(AH)	m*aktlyeklfdah	leuC	6.28	6.32	49.8	50.3	0.12	280
M82	ADVPAGVtLAEK	mtnitkrslvaagvlaalmagnvala*advpag vtlaek	oppA	6.17	6.16	58.4	55.1	0.05	380
M83	MKKTKIVaTIg(PG)	mkktkivctigp	pykF	5.98	6.12	50.7	56.0	0.07	500
M84	STEIKTQVVVL(IG)	mm*steiktqvvvlg	lpdA	6.10	6.15	50.6	49.9	0.07	380
M85	(Kms)(Ts)LVYXSEGsP	mrislkksgmlklglslvamtvaasvqa*ktlvy csegsp	dppA	5.94	6.05	57.4	51.4	0.13	660
M86	STEIKTQVVVlg	mm*steiktqvvvlg	lpdA	6.10	6.08	50.6	50.1	0.04	160
M87	MQLNSTEISELI	mqlnsteiseli	atpA	6.01	6.15	55.2	51.6	0.12	820
M88	(Si)NKPFHYQAPF	m*snkpfhyqapf	fumA	6.52	6.47	60.2	57.5	0.03	160
M89	MIKSALLVLEDG	miksallvledg	carA	6.35	6.22	41.4	41.8	0.18	620
M90	(MS)(Kg)LPIYLDYsAT	MMYGVYRA*MKLPIYLDYSAT	yhfO	6.34	6.30	45.1	41.5	0.09	180
M91-1	(AT)(IT)(EL)(LQ)(NTV)(AP)(YI)(FS)(RG)(AE) (FT)(GF)	M*TTLLNPYFGEFG	trpB	6.04	6.07	42.9	41.5	0.15	360
M91-2	(AT)(IT)(EL)(LQ)(NTV)(AP)(YI)(FS)(RG)(AE) (FT)(GF)	M*AIEQTAITRATF	argD	6.20	6.07	43.6	41.5	0.08	200
M92-1	(AM)(KL)(VX)(RS)(Le)(ME)(NK)(DI)(AK)(Id)(KY)(FX)	m*akvslekdkikf	serA	6.33	6.26	44.0	44.1	0.15	380
M92-2	(AM)(KL)(VX)(RS)(Le)(ME)(NK)(DI)(AK)(Id)(KY)(FX)	mlkremniady	glyA	6.46	6.26	45.3	44.1	0.11	300
M93	XXXXXXXXXXXX				6.30		42.5	0.05
M94	MDQTYsL(Ei)XFLN	mdqtyslesfln	gdhA	6.37	6.33	48.6	43.4	0.09	140
M95	TAPSQVLKIRRP	m*tapsqvlkirrp	pyrC	6.15	6.09	38.7	37.4	0.11	340
M96	(PA)PDM(KL)LFAGNAT	mpdmklfagnat	prsA	5.13	5.40	34.2	33.1	0.15	160
M97-1	(Sm)(LA)(IL)(NE)(VT)(PK)(AIL)(GK)(KP)(DF) (LKd)(PNl)	m*sllnvpagkdlp	ppa	4.90	5.04	19.6	21.9	0.24	500
M97-2	(Sm)(LA)(IL)(NE)(VT)(PK)(AIL)(GK)(KP)(DF) (LKd)(PNl)	m*slintkikpfkn	ahpC	4.90	5.04	20.6	21.9	0.14	300
M98	MD(It)ISVALKR(hp)s	mdiisvalkrhs	nfnB	6.17	6.17	23.9	24.1	0.22	260
M99	SKIFEDNSLTIi	m*skifednslti	cysK	5.94	6.01	34.4	33.7	0.12	240
M100	SLNFLDFEQPIA	m*slnfldfeqpia	accA	5.88	6.04	35.1	32.5	0.14	180
M101-1	(At)(Ni)(LN)(KL)(AK)(VA)(IV)(PI)(VP)(AV)(GA) (LG)	maaint*kvkkavipvagl	galU	5.00	6.06	32.3	31.4	0.08	120
M101-2	(At)(Ni)(LN)(KL)(AK)(VA)(IV)(PI)(VP)(AV)(GA) (LG)	maain*tkvkkavipvag	galU	5.00	6.06	32.4	31.4	0.09	140
M102-1	(GM)(FG)(LF)(SL)(GS)(KG)(RK)(IR)(LI)(VL) (TL)(GT)	mgflsgkrilvt	fabI	5.72	5.97	27.9	30.3	0.08	200
M102-2	(GM)(FG)(LF)(SL)(GS)(KG)(RK)(IR)(LI)(VL) (TL)(GT)	m*gflsgkrilvtg	fabI	5.72	5.97	27.7	30.3	0.09	240
M103	(VA)TYPLP(Te)DGsrL	MNMKLKTLFAAAFAVVGFCSTAS A*VTYPLPTDGSRL	ybiS	5.74	5.86	30.9	29.4	0.13	160
M104	MQQLQNIIETAF	mqqlqniietaf	dapD	5.53	5.85	29.9	30.3	0.13	420
M105	(As)VVASLKPVGFI	MKCYNITLLIFITIIGRIMLHKKTLL FAALSAALWGGATQAADA*AVVASLK PVGFI	yebL	5.58	5.80	31.2	30.8	0.08	240
M106	(MSD)D(Ig)KV(At)V(Vf)GAMs	mkrnaktiiagmialaishtama*ddikvavvg ams	livK	4.94	5.07	37.0	39.8	0.13	240
M107-1	(SM)(KE)(NI)(YK)(VH)(IT)(AD)(VP)(LA)(PQ) (GX)(DK)	M*SKNYHIAVLPGD	leuB	5.03	5.26	39.4	42.4	0.12	400
M107-2	(SM)(KE)(NI)(YK)(VH)(IT)(AD)(VP)(LA)(PQ) (GX)(DK)		NM		5.26		42.4	0.08	260
M108	TIKVGINGFGRI	m*tikvgingfgri	gapA	7.11	7.42	35.4	35.7	0.35	1660
M109-1	(Akst)(AI)I(NE)(TG)K(VL)K(Ik)AV(IN)	m*aaintkvkkavi	galU	5.00	5.34	32.8	38.3	0.07	260
M109-2	(Akst)(AI)I(NE)(TG)K(VL)K(Ik)AV(IN)		NM		5.34		38.3	0.04	140
M110-1	(MX)(LM)(KL)(XK)(FX)(RF)(GR)(MG)(FM)(SF) (LS)(DL)	mlkkfrgmfsnd	mreB	5.03	5.39	37.0	37.1	0.04	60
M110-2	(MX)(LM)(KL)(XK)(FX)(RF)(GR)(MG)(FM)(SF) (LS)(DL)	mlkkfrgmfsn	mreB	5.03	5.39	37.0	37.1	0.04	80
M111	(Mra)KVAVLGAAGGI	mkvavlgaaggi	mdh	5.56	5.69	32.3	34.4	0.20	260
M112	MTLQqqIIKALg	mtlqqqiikalg	nadE	5.33	5.70	30.6	35.5	0.11	100
M113	(SAQ)GFYXKXFLKgl	m*sgfyhkhflkl	argI	5.51	5.70	36.8	36.9	0.09	140
M114	(AQ)Xk(Td)PLYEVXt(tl)	M*aqqtplyeqhtl	gcvT	5.35	5.69	40.1	38.1	0.09	80
M115	M(NI)PS(IV)I(NVD)(LY)taip	mers*mkpsvilykalp	yiaE	6.86	5.76	40.1	36.4	0.13	160
M116	(Ap)ENK(TD)LXIYNv	mtalnkkwlsglvagalmavsvgtla*aeqktl hiynw	potF	5.35	5.82	38.2	35.7	0.10	200
M117	ARIIVVTSGKgg	m*ariivvtsgkgg	minD	5.08	5.44	29.5	28.6	0.11	300
M118-1	(AM)(QP)(TX)(PD)(ND)(IT)(LX)(IY)(VY)(EG)DE	mqtphilivede	arcA	5.08	5.40	27.3	27.7	0.10	200
M118-2	(AM)(QP)(TX)(PD)(ND)(IT)(LX)(IY)(VY)(EG)DE	MKKTAIAIAVALAGFATVAQAA PKDNTWYTGAK*	ompA	5.74	5.40	35.2	27.7	0.07	140
M119	AVAANKRSVMTL	m*avaankrsvmtl	sspA	5.10	5.42	24.2	25.3	0.19	480
M120	AETIRFATEA(GS)Y	mkkvliaaliagfslsata*aetirfateasy	artI	5.20	5.42	25.0	24.6	0.30	340
M121	(SAmgd)ERYESLFA(Qg)LK	meryeslfaqlk	trpA	5.23	5.51	28.7	26.4	0.15	280
M122	A(GVL)(VI)PVAD(PV)(LV)QXR	m*svvpvadvlq	asnS	5.04	5.33	52.4	47.8	0.11	80
M123	ENLMQVYQ(Qg)A(Rg)L	mkkllpiliglslsgfsslsqa*enlmqvyqqarl	tolC	5.09	5.29	51.5	50.3	0.08	120
M124-1	M(LIV)(QKPV)X(RF)(EK)(EQ)(MG)(VA)R(VA)(IQ)		NM		5.26		52.5	0.04	120
M124-2	M(LIV)(QKPV)X(RF)(EK)(EQ)(MG)(VA)R(VA)(IQ)		NM		5.26		52.5	0.02	60
M125	(MS)VPVQ(Kg)P(Mr)YX(Dvd)	M*SVPVQHPMYIDG	aldA	4.91	5.19	52.1	52.3	0.08	280
M126	SFNTII(Dg)PnX(PYEK)t	m*sfntiidwnsct	hisD	5.05	5.15	46.0	49.9	0.06	220
M127	SQNVYQFIDLQR	m*sqnvyqfidlqr	gltD	5.52	5.81	51.9	50.3	0.08	620
M128-1	(Sq)(QS)(VN)(VD)(YI)(LGq)(VF)(IP)DL(QP)(RE)	M*SSVDILVPDLPE	sucB	5.62	5.70	43.9	50.3	0.06	420
M128-2	(Sq)(QS)(VN)(VD)(YI)(LGq)(VF)(IP)DL(QP)(RE)	M*SQNVYQFIDLQR	gltD	5.52	5.70	51.9	50.3	0.03	180
M129	SQQ(VLR)(GI)I(FGE)D(TG)Tl(Er)	m*sqqviifdttlr	leuA	5.56	5.71	57.1	52.5	0.05	160
M130	M(QG)Q(Gd)RPVL(dr)ALL	mqqrrpvrrall	purH	5.60	5.86	57.3	56.6	0.06	160
M131	iGTE(gy)XPq(qgi)XXg		NM		5.71		62.9	0.04	80
M132	(SA)(DE)FVYTMXRV(LI)(Ga)	m*aqfvytmhrvgk	yjjK	5.24	5.67	64.9	59.4	0.03	140
M133	sEL(Lr)NXsID(lv)X(lv)	mavnllkknslalvaslllaghvqatellnssyd vsr*	cysP	6.99	6.85	35.1	31.7	0.18	60
M134	(AM)(GM)QK(VG)VSI(gp)D(di)N	mkqkvvsigdin	kdsA	6.78	6.90	30.8	30.4	0.24	200
M135	MVTFsT(NI)pXXI	mvtfhtnhgdi	ppiB	5.64	5.53	18.2	16.0	0.09	80
M136-1	(As)(Ea)(Ke)(Rk)(Nr)(In)(Fi)(Lf)(Vl)(Gv)(Pg)(Mp)	mrfqfmscraslseaglsltnslsstekm*aekrn iflvgpm	aroK	5.11	5.51	19.4	16.6	0.16	140
M136-2	(As)(Ea)(Ke)(Rk)(Nr)(In)(Fi)(Lf)(Vl)(Gv)(Pg)(Mp)	mrfqfmscraslseaglsltnslsstekm*aekrn iflvgp	aroK	5.11	5.51	19.4	16.6	0.22	200
M137	(Sm)Q(Ei)N(QT)NRK(PS)FXL	mqegqnrktssl	dksA	4.89	5.15	17.5	17.2	0.44	260
M138	TTPTFDT(Ig)EAQ(ALgr)	m*ttptfdtieaqa	fklB	4.68	4.95	22.1	23.7	0.25	340
M139	SEALKILNNIRT	m*sealkilnnirt	hns	5.25	5.70	15.4	14.1	0.74	820
M140	SKTIATENAPAA	M*SKTIATENAPAA	yjgF	5.25	5.44	13.5	14.5	0.70	1320
M141	(AMS)RHYEIVFMVXP	mrhyeivfmvhp	rpsF	5.17	5.45	15.2	14.2	0.59	560
M142	MRHYEIVFMVHP	mrhyeivfmvhp	rpsF	5.17	5.38	15.2	14.2	0.60	1120
M143	MRHYEIVFMYHP	mrhyeivfmvhp	rpsF	5.17	5.25	15.2	14.2	0.67	940
M144	MNIRPLHDRVIV	mnirplhdrviv	mopB	4.99	5.28	10.4	14.0	0.26	980
M145	AYKHILIAVDLS	m*aykhiliavdls	uspA	5.02	5.22	15.9	14.1	0.75	1660
M146-1	(ASM)(ND)(TV)(IK)(DKS)(MV)(TR)I(FNr)X(AV)		NM		4.69		14.6	0.24	200
M146-2	(ASM)(ND)(TV)(IK)(DKS)(MV)(TR)I(FNr)X(AV)		NM		4.69		14.6	0.33	260
M147-1	(AM)(ET)(SVLN)(FNq)(TQ)(TLV)(TV)(NL)(RK) (PRY)(FRE)D	M*AESFTTTNRYFD	yifE	10.85	6.54	16.2	14.1	0.52	340
M147-2	(AM)(ET)(SVLN)(FNq)(TQ)(TLV)(TV)(NL)(RK) (PRY)(FRE)D		NM		6.54		14.1	0.14	100
M148	ATYYSNDFRAGL	m*atyysndfragl	efp	4.73	5.19	20.5	23.2	0.47	1600
M149	(AS)(Drh)NNTLY(Fi)YNX(It)	mkkwsrhllaagalalgmsaahaddnntlyf ynwt*	potD	4.69	4.84	36.5	35.3	0.11	260
M150	(Ms)IIVTGGAGFIG	miivtggagfig	rfaD	4.64	4.97	34.9	36.2	0.09	220
M151	APKDNTRYTGAK	mkktaiaiavalagfatvaqa*apkdntwytg ak	ompA	5.74	5.13	35.2	27.6	0.48	1480
M152	XXXXXXXXXXXX		tufA/B	5.30	5.68	43.2	42.0	1.83
M153	XXXXXXXXXXXX		tufA/B	5.30	5.62	43.2	41.8	1.84
M154	AEIYNKDGNKVD	mmkrnilavivpallvagtana*aeiynkdgn kvd	ompF	4.48	4.71	37.1	36.4	1.35	17600