Table a2. Growth-phase, minimal-media E. coli proteins from a sonicated cell extracta
Predicted versus Observed sequence, pI, MW
Abundance
Spot
O-
N-
I.D.
N-terminal Sequence Tag
Sequence Tag Match to Genomic ORF
Locus
cpI
opI
MW
Mr
abd
abd
M155-1 (GK)(GK)(KVG)(VPK)(PVM)(MPN)

(NMI)(IVN)(VIA)(AVQ)(QAR)(RYQ)

(42)r*kgkvpmnivaq(374) tig
4.46
4.80
43.1
43.3
0.06
300
M155-2 (GK)(GK)(KVG)(VPK)(PVM)(MPN)

(NMI)(IVN)(VIA)(AVQ)(QAR)(RYQ)

(43)r*kgkvpmnivaqr(373) tig
4.46
4.80
43.1
43.3
0.10
500
M155-3 (GK)(GK)(KVG)(VPK)(PVM)(MPN)

(NMI)(IVN)(VIA)(AVQ)(QAR)(RYQ)

(44)k*gkvpmnivaqry(372) tig
4.52
4.80
43.2
43.3
0.07
340
M156 AQVINTNSLSLI m*aqvintnslsli fliC
4.34
4.57
51.2
44.2
0.26
4200
M157 MKLPVREFDAVV mklpvrefdavv sdhA
6.22
6.00
64.4
65.6
0.13
1900
M158 SEAEARPTNFIR m*seaearptnfir glnS
6.23
6.14
63.3
66.1
0.11
520
M159 KVRIDGFRKGKV (35)k*kvridgfrkgkv(381) tig
4.61
4.84
44.3
45.4
0.18
2340
M160 ATVSMRDMLKA m*atvsmrdmlkag rpsB
7.16
4.54
26.7
30.0
0.37
3540
M161 ATVSMRDMLKAG m*atvsmrdmlkag rpsB
7.16
7.14
26.7
32.2
0.43
4560
M162-1 M(IE)V(AL)(PV)(TE)(GE)(PA)(GT)A

(MG)(AF)

MIVLVTGATAGF ydfG
5.92
6.00
27.2
26.7
0.22
1300
M162-2 M(IE)V(AL)(PV)(TE)(GE)(PA)(GT)A

(MG)(AF)

NM
6.00
26.7
0.12
700
M163 MFTGSIVAIVTP mftgsivaivtp dapA
6.42
6.46
31.3
33.2
0.21
840
M164 MLEEYRKHVAER VLEEYRKHVAER yacI
5.14
5.33
93.5
99.4
0.14
1120
M165 SEKHPGPLVVEG msm*sekhpgplvveg cysI
7.63
7.16
63.9
73.2
0.07
640
M166 MELVLKDAQSAL melvlkdaqsal rplD
10.52
4.78
22.1
19.5
1.65
4960
M167-1 (SAK)IE(EN)(GT)K(LV)VIA(IV)(ND) mkiktgarilalsalttmmfsasal

a*kieegklviwin

malE
5.07
5.27
40.7
36.2
0.12
660
M167-2 (SAK)IE(EN)(GT)K(LV)VIA(IV)(ND) NM
5.27
36.2
0.07
420
M168 (SGAM)LKKFRGMFSND mlkkfrgmfsnd mreB
5.03
5.31
37.0
35.5
0.13
260
M169 MLNPIVRKFQYG mlnpivrkfqyg pnp
4.96
5.19
77.1
79.2
0.15
1000
M170 (TM)(TG)NYIFVTGGVV m*ttnyifvtggvv pyrG
5.82
5.93
60.2
57.7
0.09
480
M171 (PA)(SD)LSKEAA(LT)VTE M*PSLSKEAALVHE folE
7.35
7.20
24.7
28.5
0.34
420
M172 ADKKLVVATDTA mksvlkvslaaltlafav

ssha*a

dkklvvatdta

glnH
7.51
7.17
25.0
27.7
0.29
1440
M173 MTQDELKKAVG mtqdelkkavg rpiA
5.12
5.33
22.9
20.7
0.25
740
M174-1 M(RK)(TG)(SQ)(QE)(TY)(LX)(LG)

(SF)(TQ)(SL)(KX)

mrtsqyllstlk proS
4.98
5.16
63.7
67.4
0.05
800
M174-2 M(RK)(TG)(SQ)(QE)(TY)(LX)(LG)

(SF)(TQ)(SL)(KX)

mkgqetrgfq htpG
4.95
5.16
71.4
67.4
0.04
640
M175 SERFPNDVDPIE m*serfpndvdpie aceE
5.50
5.65
99.5
97.9
0.14
1140
M176 TILNHTLGFPRV m*tilnhtlgfprv metE
5.97
5.96
84.6
92.3
0.23
4260
M177 (Ts)DKTslsXXdd M*TDKTSLSYKDAG purM
4.65
4.84
36.7
35.4
0.12
180
M178 MKPTTISLLQXY mkpttiaslqkc panB
5.06
5.29
28.2
28.5
0.18
1080
M179 AyTTPIARYRNI m*arttpiaryrni fusA
5.13
5.40
77.4
87.0
0.22
2520
M180 MIKKIFALPVIE MKLKDCV*MIKKIFALP

VIE

yeaD
6.32
6.23
32.7
32.9
0.16
560
M181 MLEEyrkXkaa VLEEYRKHVAE yacI
5.14
5.28
93.5
98.6
0.04
140
M182 MEfTYN(Pn)QDI(IE)Q mektynpqdieq valS
5.07
5.24
108.2
103.1
0.05
120
M183 SE(DKRa)XFLVEIGTE m*sektflveigte glyS
5.18
5.41
76.7
71.7
0.06
400
M184 MIEKLXNIAIIA mieklrniaiia yihK
4.97
5.32
65.4
75.9
0.06
340
M185 TILNHTLGFP(CR)V m*tilnhtlgfprv metE
5.97
6.04
84.6
95.7
0.30
8640
M186 TILNHTLGFPRV m*tilnhtlgfprv metE
5.97
6.08
84.6
93.0
0.23
11520
M187 (ATDF)XIDDVAKQAX(SQD) MMKMRWLSAAVMLT

LYTSSSWA*FSIDD

VAKQAQS

mdoG
6.66
6.62
55.4
59.6
0.17
280
M188 MQQRRPVRRALL mqqrrpvrrall purH
5.60
5.79
57.3
54.4
0.25
640
M189 AEGFVVK(D)IHFE mamkklliasllfssatvyg*ae

gfvvkdihfe

yaeT
4.72
4.88
88.4
89.6
0.06
380
M190 MEMLSGAEMVVR memlsgaemvvr ilvI
6.30
6.16
63.0
55.0
0.12
340

a) See Table a1 for explanation of column headings.