Table a4. Periplasmic-enriched E. coli proteins from cells harvested in growth phase, minimal mediaa
Predicted versus Observed sequence, pI, MW
Abundance
Spot
O-
N-
I.D.
N-terminal Sequence Tag
Sequence Tag Match to Genomic ORF
Locusk
cpI
opI
MW
Mr
abd
abd
M195 LTLVYXSEGSPE mrislkksgmlklglslvamtvaa

svqa*ktlvycsegspe

dppA
5.94
5.87
57.4
48.9
1.21
2980
M196 ADAQKAADNKKP mkkvlgvilggllllpvvsna*ada

qkaadnkkp

hdeA
4.49
4.50
9.2
12.3
4.69
8460
M197 ENNAQTTNESAG mtmtrlkisktllavmltsavatgs

aya*ennaqttnesag

osmY
5.40
5.38
18.2
7.1
4.28
2960
M198 KDIQLLNVSYDP mnkwgvgltfllaatsvma*kdiq

llnvsydp

sbp
6.82
6.63
34.7
38.0
0.71
1240
M199 ADAQKAADNKKP mkkvlgvilggllllpvvsna*ad

aqkaadnkkp

hdeA
4.49
4.50
9.2
29.5
0.48
1060
M200 AAAEQGGFS(gd)P(sk)(ak)(tr) MKKFAAVIAVMALCSAPV*

MAAEQGGFSGPSA

yigW
4.57
4.54
12.1
4.9
6.30
600
M201 MLKREMNIADYD mlkremniadyd glyA
6.46
6.23
45.3
44.7
0.41
240
M202 APQVVDKVAAVV mknwktlllgiamiantsfa*ap

qvvdkvaavv

surA
6.54
6.33
45.1
45.1
0.50
400
M203 TELLNSXYDVSR mavnllkknslalvaslllaghvq

a*tellnssydvsr

cysP
6.99
6.63
35.1
38.0
0.71
440
M204 xDVPAGVTLAEKQ mtnitkrslvaagvlaalmagnva

la*advpagvtlaekq

oppA
6.17
6.15
58.4
51.9
0.46
500
M205 XXXXXXXXXXXX
5.49
42.9
1.08
M206 EDIKVAVVGAMS mntkgkallaglialafsnmala*

edikvavvgams

livJ
5.17
5.27
36.8
40.4
0.81
1160
M207 DDNNTLYFYNXT mkkwsrhllaagalalgmsaaha*

ddnntlyfynwt

potD
4.69
4.71
36.5
35.7
0.63
460
M208 DEGLLNKVKERG MKLAHLGRQALMGVMAV

ALVAGMSVKSFA*DEGLLNKVKERG

fliY
5.14
5.14
26.1
20.7
1.36
1060
M209 KDTIALVVSTLN mnmkklatlvsavalsatvsanama*

kdtialvvstln

rbsB
6.31
6.21
28.5
23.7
1.88
3940

a) See Table a1 for explanation of column headings.

k) An underlined locus indicates the conceptual protein does not have a recognized signal peptide which is usually associated with proteins exported across the inner membrane.