Table a5. Stationary-phase, rich-media E. coli proteins from an SDS/heat extracta
Predicted versus Observed sequence, pI, MW
Abundance
Spot
O-
N-
I.D.
N-terminal Sequence Tag
Sequence Tag Match to Genomic ORF
Locus
cpI
opI
MW
Mr
abd
abd
R1 SVIKMTDLDLAG m*svikmtdldlag pgk
4.93
5.27
41.0
39.8
0.56
1460
R2-1 (SM)(LQ)(IG)(NK)(TV)(KA)(IE)(KF)(PL)

(FA)(KM)(NR)

m*slintkikpfkn ahpC
4.90
5.16
20.6
39.7
0.32
1580
R2-2 (SM)(LQ)(IG)(NK)(TV)(KA)(IE)(KF)(PL)

(FA)(KM)(NR)

mqgsvteflkprl rpoA
4.81
5.16
36.5
39.7
0.12
560
R3 ATGKIVQVIGAV m*atgkivqvigav atpD
4.74
5.03
50.2
47.8
0.32
1300
R4 MQVSVETTQGLG mqvsvettqglg tig
4.64
4.98
48.2
51.7
0.33
600
R5 TDKLTSLRQY m*tdkltslr talB
4.94
5.18
35.1
34.9
0.29
900
R6 xxxxAGLVKELR m*aeitaslvkelr tsf
5.07
5.33
30.3
33.0
0.49
1100
R7 MENFKHLPEPFR menfkhlpepfr tnaA
6.16
6.21
52.8
49.8
0.80
7160
R8 KDTIALVVSTLN mnmkklatlvsavalsatvsanama*

kdtialvvstln

rbsB
6.31
6.31
28.5
27.9
0.83
5900
R9 ADTRIGVTIYKY mnkkvltlsavmasmlfgaaaha*

adtrigvtiyky

mglB
5.14
5.49
33.4
32.4
0.45
4720
R10 MKVAVLGAAGGI mkvavlgaaggi mdh
5.56
5.96
32.3
35.6
0.42
2380
R11 ADVPAGVTLAEK mtnitkrslvaagvlaalmagnvala*

advpagvtlaek

oppA
6.17
6.30
58.4
59.8
0.23
2360

a) See Table a1 for explanation of column headings.