Table a8. Periplasmic-enriched E. coli proteins from cells at stationary phase in rich mediaa
Predicted versus Observed sequence, pI, MW
Abundance
Spot
O-
N-
I.D.
N-terminal Sequence Tag
Sequence Tag Match to Genomic ORF
Locusk
cpI
opI
MW
Mr
abd
abd
R54 ADVPAGVTLAEK mtnitkrslvaagvlaalmagnvala

*advpagvtlaek

oppA
6.17
6.22
58.4
56.8
1.46
1300
R55 KDTIALVVSTLN mnmkklatlvsavalsatvsanama

*kdtialvvstln

rbsB
6.31
6.30
28.5
28.8
2.80
1520
R56-1 (KA)(IN)(ES)(EN)(GX)(KX)(LI)(VX)(IX)

(XA)(IX)(Nr)

mkiktgarilalsalttmmfsasala

*kieegklviwin

malE
5.07
5.25
40.7
40.9
0.94
720
R56-2 (KA)(IN)(ES)(EN)(GX)(KX)(LI)(VX)(IX)

(XA)(IX)(Nr)

NM
5.25
40.9
0.30
240
R57 DDNNTLYFYNXT mkkwsrhllaagalalgmsaaha

*ddnntlyfynwt

potD
4.69
4.73
36.5
37.3
0.85
380
R58 GLFDKLKSLV(ISV)D m*glfdklkslvsd crr
4.56
4.55
18.1
19.6
0.42
140
R59 ATVDLRIMETTD mikfsatllatliaasvna*atvdlr

imettd

cpdB
5.37
5.47
68.9
63.1
0.26
100
R60 ADTRIGVTIYKY mnkkvltlsavmasmlfgaaa

ha*adtrigvtiyky

mglB
5.14
5.21
33.4
32.7
0.78
1320
R61 AEAAKPATAADS MKSLFKVTLLATTMAVA

LHAPITFA*AEAAKPA

TAADS

fkpA
7.10
7.34
26.2
33.5
0.93
200
R62 ADKKLVVATDTA mksvlkvslaaltlafavssha*ad

kklvvatdta

glnH
7.51
7.40
25.0
27.7
1.29
460
R63 SQTVHFQGNPVT M*SQTVHFQGNPVT tpx
4.59
4.87
17.8
19.0
0.48
160
R64 AIPQNIRIGTDP MKKLVLSLSLVLAFSSATA

ALA*AIPQNIRIGTDP

hisJ
5.01
5.15
26.2
29.4
0.41
320
R65 AETIRFATEASY MKKVLIAALIAGFSLSATA*

AETIRFATEASY

artI
5.20
5.33
25.0
26.7
0.38
160
R66 ALPETVyIGTDT mkksilalsllvglstaassya*alp

etvrigtdt

argT
5.05
5.16
25.8
27.6
0.21
100
R67 DEGLLNKVKEXG MKLAHLGRQALMGVMAV

ALVAGMSVKSFA*DE

GLLNKVKERG

fliY
5.14
5.21
26.1
27.0
0.20
80
R68-1 A(QF)(Yk)(EL)(DP)(GA)(XKL)(QP)

(YX)(TA)(TX)(Ld)

MKKIWLALAGLVLAFSAS

A*AQYEDGKQYTTL

dsbA
5.39
5.49
21.1
23.4
0.46
140
R68-2 A(QF)(Yk)(EL)(DP)(GA)(XKL)(QP)

(YX)(TA)(TX)(Ld)

m*sfelpalpyakd sodB
5.88
5.49
21.1
23.4
0.05
20
R69 AVVASLXPVGFI MKCYNITLLIFITIIGRIMLH

KKTLLFAALSAALWGGATQAA

DA*AVVASLKPVGFI

yebL
5.58
5.71
31.2
32.2
0.19
60
R70 VTYPLPTDGSXL MNMKLKTLFAAAFAVVG

FCSTASA*VTYPLP

TDGSRL

ybiS
5.74
5.79
30.9
31.2
0.29
160
R71 MYIILLGAPGAG mriillgapgag adk
5.53
5.73
23.6
28.1
0.19
60
R72 MYHPLVMGNXKL mrhplvmgnwkl tpiA
5.89
5.78
27.0
27.3
0.15
60
R73 QTVPEGYQLQQV mnktliaaavagivllasnaqa*qt

vpegyqlqqv

agp
5.35
5.31
43.6
46.1
0.48
60
R74 APQVVDKVAAVV mknwktlllgiamiantsfa*apqv

vdkvaavv

surA
6.54
6.39
45.1
47.4
0.39
160
R75 KTLVYXSEGSPE mrislkksgmlklglslvamtvaas

vqa*ktlvycsegspe

dppA
5.94
5.94
57.4
53.7
0.24
40
R76 (SG)(FG)ELPALPYAKD m*sfelpalpyakd sodB
5.88
5.68
21.1
22.8
1.14
900
R77 AESVQPLEKIAP mktilpavlfaafattsawa*aes

vqplekiap

eco
6.03
6.31
16.1
14.9
0.80
260
R78 (MQ)YKTIIMPVDVF MYKTIIMPVDVF ybdQ
6.51
6.38
15.9
11.3
0.63
120
R79 (ES)ASLTGAGATFP mkvmrttvatvvaatlsmsafsvfa

*easltgagatfp

pstS
7.51
7.30
34.4
37.8
0.26
20
R80 ENLKLGFLVKQP mhkftkalaaiglaavmsqsama*

enlklgflvkqp

araF
5.60
5.76
33.2
34.8
0.26
80
R81 MKVAVLGAAGGT mkvavlgaaggi mdh
5.56
5.84
32.3
35.7
0.65
260
R82 KIEEGKLVIiIN mkiktgarilalsalttmmfsasal

a*kieegklviwin

malE
5.07
5.15
40.7
40.8
0.61
240
R83 MLDAQTIATVKA mldaqtiatvka hmpA
5.63
5.67
43.9
42.3
0.22
100
R84 DDAAIQQTLAkM mkkgfmlftllaafsgfaqa*dda

aiqqtlakm

dsbC
6.24
6.19
23.5
26.0
0.29
100
R85 AVTXLVLVyXxE M*AVTKLVLVRHGE gpmA
6.11
6.16
28.4
28.2
0.21
40
R86 (Ms)ENFKHLPEPF menfkhlpepf tnaA
6.16
6.14
52.8
49.9
0.16
40
R87 QTVPEGYQLQQV mnktliaaavagivllasnaqa*qt

vpegyqlqqv

agp
5.35
5.45
43.6
45.1
0.80
380
R88 APLTVGFSQVGS MWKRLLIVSAVSAAMSSM

ALA*APLTVGFSQVGS

ytfQ
5.68
6.08
32.1
32.2
0.49
40
R89 TDKLTSLrQxTT m*tdkltslrqytt talB
4.94
5.21
35.1
34.3
0.29
100
R90 (Ma)IKKIFALPVIE MKLKDCV*MIKKIFALPV

IE

yeaD
6.32
6.22
32.7
32.8
0.14
20
R91 YEQDKTYKITVL mkllqrgvalallttftlasetal

a*yeqdktykitvl

ushA
5.37
5.50
58.2
60.6
0.13
60
R92 SKIVKIIGREII m*skivkiigreii eno
5.23
5.55
45.5
47.3
0.15
20
R93 (STDG)ALQPDPAXQQg mqgtkirllaggllmmatagyvqa*

dalqpdpawqqg

yhjJ
5.43
5.57
53.0
52.9
0.14
40
R94 ENNAQTTNESAG mtmtrlkisktllavmltsavatg

saya*ennaqttnesag

osmY
5.40
5.47
18.2
19.9
0.22
80
R95 DAASDLKSRLDK MMKKIAITCALLSSLVASSV

WA*DAASDLKSRLDK

lolA
5.70
5.75
20.3
19.7
0.28
60
R96 MVTFHTNHGDIV mvtfhtnhgdiv ppiB
5.64
5.73
18.2
17.0
0.34
60
R97-1 (MA)(NI)(RK)(TP)(IS)(LF)(VS)(PE)

(IL)(DX)(IX)(SN)

MNSVITQKVSSGVTLYAD

TKTGGF*MNRTILVP

IDIS

ynaF
5.89
5.88
16.0
12.1
0.27
80
R97-2 (MA)(NI)(RK)(TP)(IS)(LF)(VS)(PE)

(IL)(DX)(IX)(SN)

NM
5.88
12.1
0.03
20
R98 VTGDTDQPIHiE MKFKTNKLSLNLVLASSLL

AASIPAFA*VTGDTD

QPIHIE

yhbN
7.77
5.23
17.3
14.9
0.25
60
R99 ADYKIDKFGQHA mkksllgltfaslmfsagsava*a

dykidkegqha

yceI
5.12
5.19
18.7
21.0
0.14
40
R100-1 (SM)(KFv)(IE)(FN)(DI)(TF)(VAi)(KAD)

(PI)(GA)(VD)(IP)

m*skifdfvkpgvi fba
5.75
5.74
39.0
41.7
0.12
60
R100-2 (SM)(KFv)(IE)(FN)(DI)(TF)(VAi)(KAD)

(PI)(GA)(VD)(IP)

MFENITAAPADP aspC
5.58
5.74
43.6
41.7
0.07
40
R101-1 (Tsa)(DE)(KGV)(LX)(TI)(SG)(LM)(RAD)

(QI)(DYPf)(TD)(TL)

m*tdkltslrqytt talB
4.94
5.08
35.1
36.5
0.25
60
R101-2 (Tsa)(DE)(KGV)(LX)(TI)(SG)(LM)(RAD)

(QI)(DYPf)(TD)(TL)

MKIKNILLTLCTSLLLTNV

AAHA*KEVKIGMAIDDL

xylF
4.93
5.08
33.3
36.5
0.06
20
R102-1 (ASk)(QI)(QE)(TER)(PG)(LK)(YX)(EV)

(QI)(HX)(TI)(LN)

M*AQQTPLYEQHTL gcvT
5.35
5.60
40.0
40.9
0.10
40
R102-2 (ASk)(QI)(QE)(TER)(PG)(LK)(YX)(EV)

(QI)(HX)(TI)(LN)

mkiktgarilalsalttmmfsa

sala*kieegklviwin

malE
5.07
5.60
40.7
40.9
0.02
20
R103-1 (KAs)(ED)(VT)(KX)(IT)(GX)(MV)(ATr)

(IX)(DY)(DX)(Ln)

mkiknilltlctsllltnvaaha*

kevkigmaiddl

xylF
4.93
5.09
33.3
35.0
0.20
100
R103-2 (KAs)(ED)(VT)(KX)(IT)(GX)(MV)(ATr)

(IX)(DY)(DX)(Ln)

mnkkvltlsavmasmlfgaaaha*

adtrigvtiyky

mglB
5.14
5.09
33.4
35.0
0.02
20
R104 AFTPFPDRQPTA M*AFTPFPPRQPTA ygfZ
5.01
5.22
36.0
37.4
0.12
40
R105 DDNNTxYFYNt mkkwsrhllaagalalgmsaaha

*ddnntlyfyn

potD
4.69
4.62
36.5
37.8
0.26
40
R106 STTXFvGA(Da)DXA M*STTWFVGADWLA sseA
4.40
4.45
30.7
32.9
0.14
20
R107 (Gs)KIIGIDLG(TH)TN m*gkiigidlgttn dnaK
4.67
4.81
69.0
69.6
0.06
20
R108 (AS)RTTPIArYrNI m*arttpiaryrni fusA
5.13
5.38
77.4
77.2
0.05
20
R109 ADSDIA(Dn)GQT(QTP)R MDRRRFIKGSMAMAAVC

GTSGIASLFSQAAFA*

ADSDIADGQTQR

ydcG
5.83
5.76
59.4
56.1
0.09
40
R110 ADTRIGVTIY(KG)Y mnkkvltlsavmasmlfgaaaha

*adtrigvtiyky

mglB
5.14
5.05
33.4
32.9
0.16
40
R111 XXXXXXXXXXXX
5.07
31.2
0.09
R112 (ASGt)E(PK)(Eqr)M(TG)IGAIYL MPTKMRTTRNLLLMATLL

GSALFARA*AEKEMT

IGAIYL

yphF
5.03
5.15
32.2
31.9
0.08
20
R113 (YA)EQDKT(YT)KITVL mkllqrgvalallttftlasetala

*yeqdktykitvl

ushA
5.37
5.20
58.2
30.4
0.09
20
R114 (SAMt)RVNNGLTP(QG)E(LEGD) mrvnngltpqel pckA
5.46
5.60
59.6
58.8
0.10
20
R115 AGQQQPLPVPPL mslsrrqfiqasgialcagavplka

sa*agqqqplpvppl

sufI
5.52
5.68
49.1
49.9
0.08
20
R116 AERPTLPIPtLL mqrrdflkysvalgvasalplwsra

vfa*aerptlpipdll

yacK
6.55
6.29
53.4
53.9
0.11
40
R117 ADVPAGVTLAEK mtnitkrslvaagvlaalmagnval

a*advpagvtlaek

oppA
6.17
6.08
58.4
57.0
0.65
400
R118 (STr)VDESSDNNsLL (228)a*rvdessdnnsll(245) yhjW
7.47
6.23
41.3
40.4
0.07
20
R119 (MA)DIIS(VN)ALXRXs MDIISVALKRHS nfnB
6.17
6.11
23.9
26.2
0.11
20
R120 SsPSPLNPGTNV mrkitqaisavcllfalnssaval

a*sspsplnpgtnv

yjbP
9.05
6.27
19.2
27.1
0.14
20
R121-1 (SL)A(PS)SP(SL)(PN)(LP)N(TP)

(GN)T

MRKITQAISAVCLLFALNS

SAVA*LASSPSPLNP

GT

yjbP
9.05
6.31
19.2
27.2
0.05
20
R121-2 (SL)A(PS)SP(SL)(PN)(LP)N(TP)

(GN)T

NM
6.31
27.2
0.04
20
R122 HQFETGQRVPPI mtlrkilaltclllpmmasa*hqf

etgqrvppi

ytfJ
5.68
6.42
18.2
19.7
0.33
40
R123 SYTLPSLPyaYD m*sytlpslpyayd sodA
6.98
6.85
23.0
24.6
0.19
20

a) See Table a1 for explanation of column headings.

k) An underlined locus indicates the conceptual protein does not have a recognized signal peptide, which is usually associated with proteins exported across the inner membrane.