Table a9. Inner-membrane-enriched E. coli proteins from cells at stationary phase in rich mediaa

Predicted versus Observed sequence, pI, MW
Abundance
Spot
O-
N-
I.D.
N-terminal Sequence Tag
Sequence Tag Match to Genomic ORF
Locusk
cpI
opI
MW
Mr
abd
abd
R124 ATGKIVQVIGAV m*atgkivqvigav atpD
4.74
4.73
50.2
46.7
0.28
4040
R125 SERFPNDVDPIE m*serfpndvdpie aceE
5.50
5.49
99.5
91.3
0.27
400
R126-1 (MS)(QT)(EL)(IN)(KS)T(QE)(IV)(VS)

(VE)L(IG)

mm*steiktqvvvlg lpdA
6.10
6.12
50.6
49.0
0.21
1880
R126-2 (MS)(QT)(EL)(IN)(KS)T(QE)(IV)(VS)

(VE)L(IG)

mqlnsteiseli atpA
6.01
6.12
55.2
49.0
0.21
1880
R127 (Ts)IAIVIGTHGXA m*tiaivigthgwa manX
5.87
6.12
34.9
32.1
0.42
2880
R128 MKLPVREFDAVV mklpvrefdavv sdhA
6.22
5.99
64.4
65.1
0.14
1360
R129 GKIIGIDLGTTN m*gkiigidlgttn dnaK
4.67
4.67
69.0
73.9
0.04
140
R130-1 (MG)(TK)(EI)(SI)(GF)(AI)(QD)(LX)(GF)

(ET)(TE)(NS)

MTESFAQLFEES rpsA
4.71
4.71
61.2
73.5
0.02
60
R130-2 (MG)(TK)(EI)(SI)(GF)(AI)(QD)(LX)(GF)

(ET)(TE)(NS)

m*gkiigidlgttn dnaK
4.67
4.71
69.0
73.5
0.01
20
R131 MDYTLTRIDPNG mdytltridpng nuoB
5.49
5.58
25.1
27.9
0.28
520
R132 AIEIKVPDIGAD m*aieikvpdigad aceF
4.92
4.86
66.0
73.9
0.27
1120
R133 SKIVKIIGr(ER)EI m*skivkiigrei eno
5.23
5.36
45.5
42.4
0.06
120
R134-1 (MG)(TG)(EQ)QE(TK)TSaAX(DE) MTEQEKTSAVVE hemX
4.49
4.49
43.0
42.9
0.02
40
R134-2 (MG)(TG)(EQ)QE(TK)TSaAX(DE) NM
4.49
42.9
0.03
40
R135 XXXXXXXXXXXX
4.62
29.3
0.16
R136 XXXXXXXXXXXX atpF
6.11
5.64
17.3
14.4
2.83
R137 MTLXELLVGFGT mtlkellvgfgt nuoI
5.25
5.38
20.5
23.1
0.24
60
R138 ATLTAKNLAXAY m*atltaknlakay yhbG
5.89
5.92
26.7
28.8
0.08
120
R139 ATIHVDGKEY(YE)V m*atihvdgkeyev nuoG
6.21
6.14
100.2
90.7
0.06
60

a) See Table a1 for explanation of column headings.

k) An underlined locus indicates the conceptual protein is predicted to localize in the cytoplasm.