AlignACE version 2.2 July 7, 1998 /home/amcguire/bin/alignACE -i00010_rpxx_reg_300.orf -o00010_rpxx_300.ace -a/home/amcguire/genomes/ORF_rpxx.txt -z/home/amcguire/genomes/rpxx.fna -g0.29 -x5 -e Parameter values: expect = 5 ncols = 10 npass = 1000 maxnpass = 100 nruns = 1000 maxnruns = 100 repeat = 15 maxreps = 3 nread = 500 ncycles = 1 fragment = 1 psfact = 0.1 gcback = 0.29 maxlen = 30 weight = 0.8 exclude = 1 Purged sequences: RP531 49 TRANSLATION INITIATION FACTOR IF-3 (infC) Input sequences: #1 RP257 300 CYTOCHROME C OXIDASE ASSEMBLY PROTEI (coxW) #2 RP258 126 unknown #3 RP259 56 PENICILLIN-BINDING PROTEIN 4* (pbpE) #4 RP261 92 PYRUVATE DEHYDROGENASE E1 COMPONENT, ALPHA SUBUNIT PRECURSOR (pdhA) #5 RP262 161 PYRUVATE DEHYDROGENASE E1 COMPONENT, BETA SUBUNIT PRECURSOR (pdhB) #6 RP459 300 unknown #7 RP460 146 DIHYDROLIPOAMIDE DEHYDROGENASE PRECURSOR (pdhD) #8 RP530 125 DIHYDROLIPOAMIDE ACETYLTRANSFERASE COMPONENT (pdhC) #9 RP532 300 unknown #10 RP805 249 DIHYDROLIPOAMIDE DEHYDROGENASE (pdhD) #11 RP806 300 unknown Motif number 1 AATTTCACGGTAAAAAATAACAAGATAACT 1 84 0 TAAAAAATAA 0.88851 -217 GGTGATTATGTAAAAATGAATTTACGTTAA 1 233 1 TAAAAATGAA 0.627482 -68 ATAGAAGCTAAATAATTTAAAATACTTAAC 1 258 0 AATAATTTAA 0.845408 -43 ATCAATAATAAAAAATATAAGACAA 1 286 1 AAAAATATAA 0.919729 -15 GCGATCAAGCAATAAATAATACCAAATTCA 2 55 0 AATAAATAAT 0.458801 -72 CAATACATACAATAAAATAATAAAAAGTTC 2 104 1 AATAAAATAA 0.876164 -23 CTTGTCTCTTAAAATAAATTGTATATAGC 3 10 1 TAAAATAAAT 0.741882 -47 GGTGATACAGTATAATAAATAAGCTGTGCA 5 15 0 TATAATAAAT 0.730326 -147 CAATCTAGCTAAAAATATATTAGTATATAT 5 77 0 AAAAATATAT 0.868746 -85 CTTTTTGAATAAAAATAAAATGCAGTTTCT 6 14 1 AAAAATAAAA 0.824195 -287 TTATTTTAGTTATAATATATCTCTTTTTTT 6 142 0 TATAATATAT 0.868747 -159 ATATTATAACTAAAATAAAAGATTTAATTA 6 154 1 TAAAATAAAA 0.83265 -147 TACGATATTTTAAAAAAGATTATTATAAAG 7 51 1 TAAAAAAGAT 0.657464 -96 TAGTTATAATATAATCACGTGTGT 8 5 1 TATAATATAA 0.919729 -121 GGCTTATGCAAAAAAAATAATAACATAAAT 8 95 1 AAAAAAATAA 0.882477 -31 GATTAGGCAGTAAAAATTATTACAGGATGT 9 37 1 TAAAAATTAT 0.700039 -264 TGTCTATTGCAATAAATTAAATAATGCTAT 9 64 1 AATAAATTAA 0.78198 -237 GATTTGTTACTAAAATTTATAGCATTATTT 9 82 0 TAAAATTTAT 0.780367 -219 AACAACAGCAAAAAAAAAAAAGACAATTAA 9 218 1 AAAAAAAAAA 0.754443 -83 TCGAAATGTATATAATATATTACTTTCAAC 9 265 0 TATAATATAT 0.868747 -36 ATACATTTCGAAAAATTAAAAAGGAAT 9 284 1 AAAAATTAAA 0.703646 -17 TTAAAAACTAAAAAATATAAAACAACGATA 10 24 0 AAAAATATAA 0.919729 -226 CCTTTACTGAAAAAATATATAGAATTTAGT 10 113 0 AAAAATATAT 0.868746 -137 CCTTTTTTGGAATAATTTATACCTTAAAAT 10 207 1 AATAATTTAT 0.759618 -43 ATTTATACCTTAAAATAGAACCAATAAAAT 10 221 1 TAAAATAGAA 0.83526 -29 AAATAGAACCAATAAAATAAAAATAAA 10 233 1 AATAAAATAA 0.876164 -17 AATTAAGTACTAAAAAAGAATAGAAGT 11 8 0 TAAAAAAGAA 0.768663 -293 AATTACCATATATAAAATAATATGACGGAA 11 37 0 TATAAAATAA 0.882477 -264 ACTATTAATTAATAATATAATTATAGCTAA 11 132 0 AATAATATAA 0.915226 -169 CAAAATGATATATAAATTAACTATTAATTA 11 151 0 TATAAATTAA 0.791954 -150 TTTTTAATTAAATAATTAATATATAATAGA 11 202 1 AATAATTAAT 0.563941 -99 ATAATTAATATATAATAGAAAATTATATAA 11 213 1 TATAATAGAA 0.826908 -88 TAGAAAATTATATAATTTAATATCGCTTGA 11 228 1 TATAATTTAA 0.852942 -73 ********** Masking position 5 Map Score: 36.2915 Number of sites scoring better than the average of aligned sites = 1963 Number in coding regions = 1200 Number in noncoding regions = 763 Number of orfs with sites within 600 bp upstream = 293 Fraction of orfs with sites within 600 bp upstream = 0.0470607 Motif number 2 TATCTAAACTTATATAAGTTTTTTTTGTTCC 1 125 1 TATATAATTT 0.829384 -176 AGCTAAATAATTTAAAATACTTAACGTAAAT 1 252 0 TTTAAAAACT 0.884643 -49 TTGCTGACACTTTAAAAATATTCTCAGTATC 6 48 0 TTTAAAATAT 0.74167 -253 GCCTATTAAATATATAACTCTCTCACTTGTT 6 185 0 TATATAATCT 0.746083 -116 TGTTTATAATATTGTAGTATTGT 7 3 1 TTTATAAATT 0.945244 -144 GTATCTAATCTTTATAATAATCTTTTTTAAA 7 59 0 TTTATAAAAT 0.868052 -88 GTTACTAAAATTTATAGCATTATTTAATTTA 9 76 0 TTTATAGATT 0.846395 -225 ACAACAAGACTTTATAAATTTAGTATAATCA 9 120 1 TTTATAATTT 0.911141 -181 ATATACTAGTTTTATAAAACTACGTTTCATT 9 150 0 TTTATAAACT 0.912566 -151 ACTTTTCATATTTATGATATTCTTTAGCTCT 9 183 0 TTTATGAATT 0.64676 -118 TTTGCTGTTGTTTAAAACTTTTCATATTTAT 9 199 0 TTTAAAATTT 0.882821 -102 TTTCAACTATTTTAAAATAATCAATTAATTG 9 241 0 TTTAAAAAAT 0.828614 -60 AGTTAAGAAATATAAAACATTTAGCTCTTTT 10 71 1 TATAAAAATT 0.857442 -179 ACTGAAAAAATATATAGAATTTAGTAGATGA 10 107 0 TATATAGATT 0.723167 -143 AACCTCAGCATTTAAAGAACTTTATCAACAC 10 153 0 TTTAAAGACT 0.709908 -97 AATTACCATATATAAAATAATATGACGGAAA 11 36 0 TATAAAAAAT 0.696239 -265 TTCCTTTGTATTTAAAAATTTTTAATTAAAT 11 184 1 TTTAAAATTT 0.882821 -117 GATATTAAATTATATAATTTTCTATTATATA 11 221 0 TATATAATTT 0.829383 -80 ACTTTTTGAGTTTATAACATTTCTTTAGATA 11 260 0 TTTATAAATT 0.945257 -41 ******* *** Masking position 3 Map Score: 19.4918 Number of sites scoring better than the average of aligned sites = 913 Number in coding regions = 546 Number in noncoding regions = 367 Number of orfs with sites within 600 bp upstream = 175 Fraction of orfs with sites within 600 bp upstream = 0.0281079 Motif number 3 ATAATACTTATGAACATGCGATATATCACCTCAATACT 1 163 1 TAAACGATCA 0.985212 -138 AATCAAGTTATAAAGTTACGGCATGACATTTGTTTCTG 4 41 0 TAATCGATCA 0.979618 -52 GATTGCATTGTTAATTTACGTGATTACATTATAAGTAA 5 102 1 TAATCGATCA 0.984022 -60 TTTTAAAGTGTCAGCAAACGTCATCATATTAATATAGC 6 62 1 TAGACGATTA 0.886472 -239 TAATCTTTTTTAAAATATCGTAGTCACACAACAGGCAT 7 35 0 TAATCGGTCA 0.930585 -112 GATACCGTAGTCAATATACGGTATTACACATCATAAGG 7 85 1 TAAACGATCA 0.988886 -62 GAACACAGTGTCAACACACGTGATTATATTATAACTA 8 10 0 TAAACGATTA 0.967021 -116 TAAATTTTAGTAACAAATCGTAATACCACAACAAGACT 9 93 1 TACACGATCA 0.958523 -208 TTTTTAGTACTTAATTTCCGTCATATTATTTTATATAT 11 22 1 TAATCGATTA 0.956776 -279 * ** * ** ** ** Masking position 14 Map Score: 8.61688 Number of sites scoring better than the average of aligned sites = 22 Number in coding regions = 13 Number in noncoding regions = 9 Number of orfs with sites within 600 bp upstream = 8 Fraction of orfs with sites within 600 bp upstream = 0.00128493 Motif number 4 TTTGTGTACCTAATACTGATTCAGTTATCT 1 62 1 TAATACTGAT 0.739626 -239 CCCGAAGATATAATACTTATGAACATGCGA 1 154 1 TAATACTTAT 0.947396 -147 TATATCACCTCAATACTTAAGTAAATATAA 1 184 1 CAATACTTAA 0.841468 -117 AAAGTGTCCTTAATGCTTATATTTACTTAA 1 200 0 TAATGCTTAT 0.789738 -101 AAATAATTTAAAATACTTAACGTAAATTCA 1 249 0 AAATACTTAA 0.863296 -52 TTGATTACAATAATTCTTAAGGAGTAATTA 4 73 1 TAATTCTTAA 0.909262 -20 TTTGATAATCTGATACTTAATTATCAATAC 6 115 0 TGATACTTAA 0.816362 -186 ATAAATAAGCTATTACTTATTCCTGCTACT 6 244 0 TATTACTTAT 0.817141 -57 CAATACATACTAGTACTTAAAAACTAAAAA 10 40 0 TAGTACTTAA 0.91538 -210 AAATGTTTTATATTTCTTAACTGCAATACA 10 63 0 TATTTCTTAA 0.713168 -187 TATTCTTTTTTAGTACTTAATTTCCGTCAT 11 16 1 TAGTACTTAA 0.91538 -285 TATTATTAATTAATAGTTAATTTATATATC 11 145 1 TAATAGTTAA 0.733439 -156 ATATCCTCAAATTCTTATGAACTTTTTG 11 283 0 AAATTCTTAT 0.589265 -18 ********** Masking position 9 Map Score: 7.56177 Number of sites scoring better than the average of aligned sites = 206 Number in coding regions = 112 Number in noncoding regions = 94 Number of orfs with sites within 600 bp upstream = 56 Fraction of orfs with sites within 600 bp upstream = 0.00899454 Motif number 5 CTAAACTTATATAAGTTTTTTTTGTTCCCGAAG 1 128 1 AAATTTTTTT 0.916364 -173 TTAGAACTTTTTATTATTTTATTGT 2 112 0 AAATTTTATT 0.948964 -15 TCGGAGCTATATACAATTTATTTTAAGAGACAA 3 12 0 AACATTTTTT 0.710222 -45 AAGTTACGGCATGACATTTGTTTCTGGATTCCT 4 34 0 AGAATTTTTT 0.710428 -59 TAAGTAAATGAAAATATTTAATTTAAATAAAAA 5 133 1 AAAATTTATT 0.956693 -29 TAACTAAAATAAAAGATTTAATTAAAACAAGTG 6 160 1 AAAATTTATT 0.956644 -141 GCTGTTGTTTAAAACTTTTCATATTTATGATAT 9 194 0 AAATTTTATA 0.702612 -107 TATCTAATACAAAACATTTGATTGTCTTGAATT 11 63 0 AAAATTTATT 0.956644 -238 TTTGTATTTAAAAATTTTTAATTAAATAATTAA 11 188 1 AAATTTTATT 0.948964 -113 ATTAAATTATATAATTTTCTATTATATATTAAT 11 216 0 AAATTTCATT 0.77786 -85 TTTTGAGTTTATAACATTTCTTTAGATACTTCA 11 255 0 AAAATTTTTT 0.928705 -46 * ** **** *** Masking position 1 Map Score: 7.44972 Number of sites scoring better than the average of aligned sites = 410 Number in coding regions = 248 Number in noncoding regions = 162 Number of orfs with sites within 600 bp upstream = 91 Fraction of orfs with sites within 600 bp upstream = 0.0146161 Motif number 6 TCTTCGGGAACAAAAAAAACTTATATAAGTT 1 131 0 CAAAAAAACT 0.785207 -170 ACTATGTAAACAATACAAACTTCACCACAAA 2 16 0 CAATAAAACT 0.953269 -111 TGCGATCAAGCAATAAATAATACCAAATTCA 2 55 0 CAATAATAAT 0.861543 -72 GTGGCAGAATCAATACATACAATAAAATAAT 2 94 1 CAATAATACA 0.908973 -33 TCAGTCAAACCAATATATACTAATATATTTT 5 65 1 CAATAATACT 0.98237 -97 TTCTTTAGCTCTATATATACTAGTTTTATAA 9 164 0 CTATAATACT 0.953269 -137 TTCTTAACTGCAATACATACTAGTACTTAAA 10 49 0 CAATAATACT 0.98237 -201 TATAACATTTCTTTAGATACTTCAAGCGATA 11 248 0 CTTTAATACT 0.785207 -53 ***** ***** Masking position 5 Map Score: 6.47351 Number of sites scoring better than the average of aligned sites = 40 Number in coding regions = 25 Number in noncoding regions = 15 Number of orfs with sites within 600 bp upstream = 7 Fraction of orfs with sites within 600 bp upstream = 0.00112432 Motif number 7 AGCTTCTATCAATAATAAAAAATATAAGACAA 1 279 1 AATATAAAAA 0.800905 -22 TCAAGCAATAAATAATACCAAATTCATTTCTCGA 2 47 0 AATATACAAT 0.67201 -80 CATACAATAAAATAATAAAAAGTTCTAA 2 109 1 AATATAAAGT 0.702845 -18 CTTAATTATCAATACGCCATGAATGGGTTTGCTA 6 96 0 AATAGCATAT 0.867006 -205 TTATATATTTAATAGGCTAAAATTACAAGATCAC 6 199 1 AATAGCAAAT 0.937063 -102 ATGCTTTATAAATAAGCTATTACTTATTCCTGCT 6 247 0 AATAGCATAT 0.853568 -54 CAGGCATTACAATACTACAATATTATAAACA 7 8 0 AATATAAAAT 0.935534 -139 CATAAGGTTAAATATGCCAAGAATTTGATTTA 7 125 1 AATAGCAAAT 0.937923 -22 TAAACCTTATAATACTAGAACACAGTGTCAACAC 8 31 0 AATATAAAAA 0.801303 -95 TTGTTAGAAGAATAAGATATGATAT 9 2 0 AATAGAATAA 0.685076 -299 GAATATCATAAATATGAAAAGTTTTAAACAACAG 9 192 1 AATAGAAATT 0.780041 -109 ACTATTTTAAAATAATCAATTAATTGTCTTTTTT 9 233 0 AATATCATAT 0.835202 -68 TCGAAATGTATATAATATATTACTTTCAACTATT 9 261 0 TATATAATAT 0.516377 -40 AAATAGAACCAATAAAATAAAAATAAA 10 233 1 AATAAAAAAT 0.710399 -17 ATATGCTTCAAATAGGATAATACTAAGAAAATTA 11 94 0 AATAGAAAAT 0.955572 -207 ACTATTAATTAATAATATAATTATAGCTAAATTA 11 128 0 AATATAAATT 0.736082 -173 ATAATTAATATATAATAGAAAATTATATAATTTA 11 213 1 TATATAAAAT 0.709803 -88 **** ** ** * * Masking position 4 Map Score: 10.6303 Number of sites scoring better than the average of aligned sites = 534 Number in coding regions = 342 Number in noncoding regions = 192 Number of orfs with sites within 600 bp upstream = 103 Fraction of orfs with sites within 600 bp upstream = 0.0165435 Motif number 8 AATTATGATAATCTATATCTAAACTTATATAA 1 110 1 ATCTATCTAA 0.706734 -191 AATTATTGTAATCAAGTTATAAAGTTACGGCA 4 56 0 ATCAATATAA 0.760485 -37 TAACAATGCAATCTAGCTAAAAATATATTAGT 5 83 0 ATCTATAAAA 0.880326 -79 AAATATTTTCATTTACTTATAATGTAATCACG 5 120 0 ATTTATATAA 0.951853 -42 AAAATATTTAATTTAAATAAAAAGGGAAA 5 143 1 ATTTATAAAA 0.887727 -19 AAATCTTTTATTTTAGTTATAATATATCTCTT 6 147 0 TTTTATATAA 0.685639 -154 AAAATAAAAGATTTAATTAAAACAAGTGAGAG 6 165 1 ATTTATAAAA 0.887727 -136 AAGCATATTAATTTAGCTATAATTATATTATT 11 120 1 ATTTATATAA 0.951853 -181 TTAATAGTTAATTTATATATCATTTTGCTATT 11 154 1 ATTTATATCA 0.793683 -147 TATATTAATTATTTAATTAAAAATTTTTAAAT 11 193 0 ATTTATAAAA 0.887727 -108 TAATTAAATAATTAATATATAATAGAAAATTA 11 206 1 ATTAATATAA 0.835203 -95 ***** ***** Masking position 5 Map Score: 8.73119 Number of sites scoring better than the average of aligned sites = 186 Number in coding regions = 112 Number in noncoding regions = 74 Number of orfs with sites within 600 bp upstream = 45 Fraction of orfs with sites within 600 bp upstream = 0.00722775 Motif number 9 ATCTAAACTTATATAAGTTTTTTTTGTTCC 1 126 1 ATATAAGTTT 0.408757 -175 CAATAAAATAATAAAAAGTTCTAA 2 113 1 ATAAAAAGTT 0.688279 -14 TGTAATCAAGTTATAAAGTTACGGCATGAC 4 52 0 TTATAAAGTT 0.92167 -41 TTATTTTTATTCAAAAAGCTC 6 2 0 TCAAAAAGCT 0.558524 -299 TGCTGACACTTTAAAAATATTCTCAGTATC 6 48 0 TTAAAAATAT 0.531579 -253 AAAAAGATTATTATAAAGATTAGATACCGT 7 63 1 TTATAAAGAT 0.706161 -84 ATCATAAGGATCATAAGGTTAAATATGCCA 7 114 1 TCATAAGGTT 0.830122 -33 TGTTCTAGTATTATAAGGTTTATATCCTGA 8 43 1 TTATAAGGTT 0.88639 -83 CAACAAGACTTTATAAATTTAGTATAATCA 9 121 1 TTATAAATTT 0.847474 -180 ATCATAAATATGAAAAGTTTTAAACAACAG 9 196 1 TGAAAAGTTT 0.697658 -105 TTTGAAAAATTTGTATCGTTGT 10 3 1 TGAAAAATTT 0.776786 -247 AATCAAGTGTTGATAAAGTTCTTTAAATGC 10 147 1 TGATAAAGTT 0.88052 -103 AATTATTTAATTAAAAATTTTTAAATACAA 11 189 0 TTAAAAATTT 0.847474 -112 TGTTATAAACTCAAAAAGTTCATAAGAATT 11 272 1 TCAAAAAGTT 0.88052 -29 ********** Masking position 5 Map Score: 6.90545 Number of sites scoring better than the average of aligned sites = 461 Number in coding regions = 291 Number in noncoding regions = 170 Number of orfs with sites within 600 bp upstream = 99 Fraction of orfs with sites within 600 bp upstream = 0.0159011 Motif number 10 AATACTTATGAACATGCGATATATCACCTC 1 165 1 AACATGCGAT 0.960628 -136 TGCCACTTTGAACATGCGATCAAGCAATAA 2 70 0 AACATGCGAT 0.960628 -57 GAATACAGTGTACAAGAGATGGGGTGATAC 5 37 0 TACAAGAGAT 0.795563 -125 AGATTATCAAAAAAAGAGATATATTATAAC 6 134 1 AAAAAGAGAT 0.909351 -167 AATCTATGGTAAAATGAGATAACCTCAGCA 10 174 0 AAAATGAGAT 0.949282 -76 AAGGAATAGCAAAATGATATATAAATTAAC 11 160 0 AAAATGATAT 0.743296 -141 ********** Masking position 4 Map Score: 1.2448 Number of sites scoring better than the average of aligned sites = 28 Number in coding regions = 16 Number in noncoding regions = 12 Number of orfs with sites within 600 bp upstream = 8 Fraction of orfs with sites within 600 bp upstream = 0.00128493 Motif number 11 AGGAATCCAGAAACAAATGTCATGCCGTAA 4 34 1 AAACAAATGT 0.94422 -59 GATTTAATTAAAACAAGTGAGAGAGTTATA 6 174 1 AAACAAGTGA 0.809302 -127 GTTATAATATAATCACGTGTGTTGACACTG 8 13 1 AATCACGTGT 0.891188 -113 TAGTATAATCAATGAAACGTAGTTTTATAA 9 140 1 AATGAAACGT 0.741061 -161 TAAAGGTTAAAATCAAGTGTTGATAAAGTT 10 137 1 AATCAAGTGT 0.96379 -113 AATTCAAGACAATCAAATGTTTTGTATTAG 11 63 1 AATCAAATGT 0.956601 -238 TGAAGTATCTAAAGAAATGTTATAAACTCA 11 255 1 AAAGAAATGT 0.877195 -46 ********** Masking position 5 Map Score: 1.59482 Number of sites scoring better than the average of aligned sites = 27 Number in coding regions = 18 Number in noncoding regions = 9 Number of orfs with sites within 600 bp upstream = 5 Fraction of orfs with sites within 600 bp upstream = 0.000803084 Motif number 12 ********** No masking Map Score: 3.98147e-12 Number of sites scoring better than the average of aligned sites = 0 Number in coding regions = 0 Number in noncoding regions = 0 Number of orfs with sites within 600 bp upstream = 0 Fraction of orfs with sites within 600 bp upstream = 0 Motif number 13 ATAACTGAATCAGTATTAGGTACACAAACTA 1 59 0 CAGTATTAGT 0.725513 -242 CGCATGTTCAAAGTGGCAGAATCAATACATA 2 82 1 AAGTGGCAGA 0.94853 -45 AAAATATTCTCAGTATCAATAAGAAACTGCA 6 34 0 CAGTATCAAA 0.927421 -267 AATATTTTTAAAGTGTCAGCAAACGTCATCA 6 57 1 AAGTGTCAGA 0.964771 -244 ATTGATAATTAAGTATCAGATTATCAAAAAA 6 117 1 AAGTATCAGT 0.90797 -184 CGTAATAATTCAGTAGCAGGAATAAGTAATA 6 233 1 CAGTAGCAGA 0.96925 -68 TACTAGAACACAGTGTCAACACACGTGATTA 8 22 0 CAGTGTCAAA 0.939139 -104 ********* * Masking position 8 Map Score: 0.128775 Number of sites scoring better than the average of aligned sites = 79 Number in coding regions = 63 Number in noncoding regions = 16 Number of orfs with sites within 600 bp upstream = 11 Fraction of orfs with sites within 600 bp upstream = 0.00176678 Motif number 14 ********** No masking Map Score: 3.98147e-12 Number of sites scoring better than the average of aligned sites = 0 Number in coding regions = 0 Number in noncoding regions = 0 Number of orfs with sites within 600 bp upstream = 0 Fraction of orfs with sites within 600 bp upstream = 0 Motif number 15 ********** No masking Map Score: 3.98147e-12 Number of sites scoring better than the average of aligned sites = 0 Number in coding regions = 0 Number in noncoding regions = 0 Number of orfs with sites within 600 bp upstream = 0 Fraction of orfs with sites within 600 bp upstream = 0