AlignACE version 2.2 July 7, 1998 /home/amcguire/bin/alignACE -i250_fts_cell_wall_synecho_reg_100.orf -o250_synecho_100.ace -a/home/amcguire/alignace/lib/ORF_synecho.txt -z/skink1/amcguire/genomes/synecho.fna -g0.48 -x5 -e Parameter values: expect = 5 ncols = 10 npass = 1000 maxnpass = 100 nruns = 1000 maxnruns = 100 repeat = 15 maxreps = 3 nread = 500 ncycles = 1 fragment = 1 psfact = 0.1 gcback = 0.48 maxlen = 30 weight = 0.8 exclude = 1 Input sequences: #1 RCY09804 120 Synechocystis #2 RCY06805 249 Synechocystis #3 RCY33828 162 Synechocystis #4 RCY50530 178 Synechocystis #5 RCY34375 180 Synechocystis #6 RCY29867 300 Synechocystis #7 RCY07516 147 Synechocystis #8 RCY07197 190 Synechocystis #9 RCY14422 284 Synechocystis #10 RCY11010 269 Synechocystis #11 RCY50049 71 Synechocystis #12 RCY41993 184 Synechocystis Motif number 1 GAGCCATGGACTCCTCCCACCCAAAACTAAATT 1 18 1 CTCCCCACCA 0.945902 -103 AATTCATCTCCCTTTACCAAGCATATTTCCCGT 1 62 1 CCTACCAGCA 0.942819 -59 TATTTTTACCCTCCACTCCAGCCATTCTCCCGT 2 11 1 CTCCTCCGCC 0.961473 -239 ACAGAGATCCCCTCGCCCAAGCACAAATCCCAG 2 115 0 CCTCCCAGCA 0.987999 -135 TCCCCTAATACTTCCCCCCTGCA 2 237 1 CTTCCCCGCA 0.991472 -13 CTCCACGGAACTTATCCCCGGCAAAGTAATTCA 3 101 0 CTTCCCCGCA 0.991472 -62 CCATGTCCCCCTTGCCCCCTGCCGTTGGCCTGA 4 22 0 CTTCCCCGCC 0.993407 -157 CCACTGTTATCTATGCCCATGTCCCCCTTGCCC 4 38 0 CTACCCAGTC 0.727611 -141 CGCCAACTAACCAAAACCCCCCCAGGGATAAAG 5 59 0 CCAACCCCCC 0.925309 -122 TGAGCAGTTGCCCATTCCCACCATTGGCATTGG 9 32 1 CCCTCCCCCA 0.795866 -253 TCCGCCGTCACCAAAACCTGGCCGTCACACTGG 9 74 0 CCAACCTGCC 0.767754 -211 AACAACCGCCCTTTGCTCCGGCCTATCTGAATT 9 127 1 CTTCTCCGCC 0.955641 -158 TTAGTTTGTTCCCTAACCCCGCCGGGGAAATTG 9 216 0 CCCACCCGCC 0.982549 -69 AACAGGCTGCCCAGGCTCCAGCAATGACCATAA 9 258 1 CCACTCCGCA 0.95852 -27 AGCAAAAACTCTACCCCCAGGCATAGACAAAAC 10 166 0 CTACCCAGCA 0.97784 -104 AGCAACGCTCCTCACAAGCCATAAATGTTA 10 250 0 CTCCACAGCC 0.812825 -20 ACGGAGGAATCCTGACCCACCCAAAGATGGTTA 12 55 0 CCTCCCACCA 0.96049 -130 ATCAAAATGTCCAATCCCCCGCATAACGGAGGA 12 80 0 CCACCCCGCA 0.993851 -105 *** **** *** Masking position 8 Map Score: 30.5029 Number of sites scoring better than the average of aligned sites = 2257 Number in coding regions = 2103 Number in noncoding regions = 154 Number of orfs with sites within 600 bp upstream = 169 Fraction of orfs with sites within 600 bp upstream = 0.0271442 Motif number 2 CAAAAGCAACCACAGGGAGAGCAACGCTTCCGAAG 2 177 0 CACAGGGAAC 0.904817 -73 AGCGACTAGGCGCATGGTTATTAGCTCACGTCCCT 3 19 0 CGCATGGAAC 0.640627 -144 GAATTACTTTGCCGGGGATAAGTTCCGTGGAGGCT 3 102 1 GCCGGGGATC 0.87227 -61 ATCCCGCCTTCCCAGGGTAACAGCCTCCACGGAAC 3 123 0 CCCAGGGAGC 0.995556 -40 CCAAAACCCCCCCAGGGATAAAGCCGGGATATTTC 5 47 0 CCCAGGGAGC 0.995561 -134 TACGGGGCGACCCATTGACAGCGACACCAACATTC 7 33 0 CCCATTGAGC 0.93249 -115 TTCGTCCCTTCCCAGTTGGAAAAACCCCAGTGGCA 8 140 1 CCCAGTTAAC 0.882864 -51 TTGGAAAAACCCCAGTGGCAAAACCTTACTAATCC 8 155 1 CCCAGTGAAC 0.98482 -36 TGGGCAACTGCTCAGTGATACTAGCCGCTAGTTCG 9 11 0 CTCAGTGAAC 0.900039 -274 GGGGCAGGGCCCCAGTGTGACGGCCAGGTTTTGGT 9 63 1 CCCAGTGAGC 0.991743 -222 GGCGGATTTACTCGGTTTGACAGTCAAACAACCGC 9 101 1 CTCGGTTAGC 0.563484 -184 ATTCAGATAGGCCGGAGCAAAGGGCGGTTGTTTGA 9 124 0 GCCGGAGAGC 0.871856 -161 CCCTAACCCCGCCGGGGAAATTGTCTTTGTCGCAC 9 204 0 GCCGGGGAGC 0.983747 -81 AAACCCAGAGGCCACGGTAAACAGCGACGGAGAGA 10 135 0 GCCACGGAAC 0.840534 -135 ******* * * * Masking position 10 Map Score: 17.0772 Number of sites scoring better than the average of aligned sites = 1548 Number in coding regions = 1432 Number in noncoding regions = 116 Number of orfs with sites within 600 bp upstream = 139 Fraction of orfs with sites within 600 bp upstream = 0.0223257 Motif number 3 CCCCTGTTGTTGGGTTTTTT 1 111 0 CCCCTGTTGT 0.850917 -10 CAAGCACAAATCCCAGCGGTCTATCGTCCC 2 101 0 TCCCAGCGGT 0.982388 -149 AGCGTTGCTCTCCCTGTGGTTGCTTTTGCG 2 184 1 TCCCTGTGGT 0.9784 -66 TCCCCCTTGCCCCCTGCCGTTGGCCTGATC 4 20 0 CCCCTGCCGT 0.817734 -159 CCCGGCTTTATCCCTGGGGGGGTTTTGGTT 5 54 1 TCCCTGGGGG 0.97708 -127 GCGACATCGATGCCAGGGGCGTTCCCTTTT 5 118 1 TGCCAGGGGC 0.910805 -63 CTAGATCACCTCCCATGGGTGCTTGCATCA 6 73 0 TCCCATGGGT 0.85854 -228 ACACCAACATTCCCTTTGGTCAGGATAGTT 7 15 0 TCCCTTTGGT 0.84024 -133 CTTCGTCCCTTCCCAGTTGGAAAAACCCCA 8 139 1 TCCCAGTTGG 0.905421 -52 GTTGGAAAAACCCCAGTGGCAAAACCTTAC 8 154 1 CCCCAGTGGC 0.969223 -37 CCTGCCCCAATGCCAATGGTGGGAATGGGC 9 41 0 TGCCAATGGT 0.595368 -244 TGGGGCAGGGCCCCAGTGTGACGGCCAGGT 9 62 1 CCCCAGTGTG 0.834901 -223 AAGACAATTTCCCCGGCGGGGTTAGGGAAC 9 212 1 CCCCGGCGGG 0.950731 -73 GTTTTGTCTATGCCTGGGGGTAGAGTTTTT 10 166 1 TGCCTGGGGG 0.956284 -104 TTTGTTACTCCGCCAGCGGGTGGGGTATTT 12 112 1 CGCCAGCGGG 0.968992 -73 ********** Masking position 4 Map Score: 17.8008 Number of sites scoring better than the average of aligned sites = 2535 Number in coding regions = 2353 Number in noncoding regions = 182 Number of orfs with sites within 600 bp upstream = 188 Fraction of orfs with sites within 600 bp upstream = 0.030196 Motif number 4 TTGTTGGGTTTTTTGTCTGTGCCTTTCCTGA 1 94 0 TTTTTCTGTG 0.981417 -27 ATGGTTTGCATTTTCTGTTTGAATTACTTTG 3 82 1 TTTTTGTTTG 0.721679 -81 TGCGTCCCACTGTTATCTATGCCCATGTCCC 4 46 0 TGTTTCTATG 0.874118 -133 AAAAGGGGAATTTTTTCTGTAACTGACAGCT 4 95 1 TTTTTCTGTA 0.821879 -84 TGTCGCACCATTTTAGCTTTGAGGGGGATCA 5 93 0 TTTTGCTTTG 0.897152 -88 CATTTGCAATTTTTATCTGTGATCTAGATCA 6 95 0 TTTTTCTGTG 0.981417 -206 TGAACTAATTTTTTGACTATGGCCAACGCAA 8 105 1 TTTTACTATG 0.856466 -86 GGCCTCTGGGTTTTGTCTATGCCTGGGGGTA 10 157 1 TTTTTCTATG 0.970843 -113 GGGGGTAGAGTTTTTGCTATGATGTCGCTCA 10 181 1 TTTTGCTATG 0.942115 -89 GGGTGGGGTATTTTTTTTGTGGGCTTATCAA 12 129 1 TTTTTTTGTG 0.838124 -56 **** ****** Masking position 4 Map Score: 8.64105 Number of sites scoring better than the average of aligned sites = 184 Number in coding regions = 144 Number in noncoding regions = 40 Number of orfs with sites within 600 bp upstream = 48 Fraction of orfs with sites within 600 bp upstream = 0.0077096 Motif number 5 GGCTGGAGTGGAGGGTAAAAATA 2 4 0 GAGGGTAAAA 0.912412 -246 TCCCGTGATAGAGGGAAAAAGCTCAGATTC 2 38 1 GAGGGAAAAA 0.963398 -212 CAAATTCTTAGGGCGCAAAAGCAACCACAG 2 197 0 GGGCGCAAAA 0.962658 -53 GAAGTATTAGGGGAGCAAAATGAGCAAATT 2 221 0 GGGAGCAAAA 0.945634 -29 GCCAACGGCAGGGGGCAAGGGGGACATGGG 4 26 1 GGGGGCAAGG 0.927661 -153 TGCCCAATCAAGCGGCAAAAAATCTTAAAT 5 11 1 AGCGGCAAAA 0.850544 -170 ATTGAAAGTTGGGGGCAAAAAGGGAACGCC 5 135 0 GGGGGCAAAA 0.994395 -46 GAAAGAATAGGAGTGAAAAATATGGCGGAG 6 162 0 GAGTGAAAAA 0.720699 -139 GAAAAACCCCAGTGGCAAAACCTTACTAAT 8 158 1 AGTGGCAAAA 0.793376 -33 CTAAAACAGAGAGGGCAAGACGAAC 10 6 0 GAGGGCAAGA 0.972828 -264 GACGGAGAGAAAGGGAAAAAGATTCCCACC 10 115 0 AAGGGAAAAA 0.867019 -155 ********** Masking position 7 Map Score: 10.1347 Number of sites scoring better than the average of aligned sites = 1382 Number in coding regions = 1202 Number in noncoding regions = 180 Number of orfs with sites within 600 bp upstream = 192 Fraction of orfs with sites within 600 bp upstream = 0.0308384 Motif number 6 CTACGTCCCCAAACGATCCCGCCTTCCCA 3 144 0 CCAACGATCC 0.955914 -19 TTAAATTGAGCCGAAATATCCCGGCTTTATC 5 35 1 CCAAATATCC 0.955008 -146 GGGGATCACCGCCAACTAACCAAAACCCCCC 5 70 0 GCAACTAACC 0.937938 -111 CCTTTTTGCCCCCAACTTTCAATAGTTTTTA 5 142 1 CCAACTTTCA 0.880733 -39 CCCATGAACTCCAAACTTTCCCTATTTGACA 6 191 1 CCAACTTTCC 0.986887 -110 TTGTCTGTTACCAATCTTTCCGAGGATATAC 6 248 1 CCATCTTTCC 0.922326 -53 GCTGGGCAAACTAACCTAGGGTTATC 11 6 1 GCAACTAACC 0.937923 -66 TTCCTGTCCCGCTAATTTTCCGGACCAACCG 11 49 1 GCAATTTTCC 0.833307 -23 CTTGCCCAAATATCCGTTAAAAACT 12 170 0 CCAAATATCC 0.955008 -15 ** ******** Masking position 4 Map Score: 7.85403 Number of sites scoring better than the average of aligned sites = 330 Number in coding regions = 282 Number in noncoding regions = 48 Number of orfs with sites within 600 bp upstream = 54 Fraction of orfs with sites within 600 bp upstream = 0.00867331 Motif number 7 GGTTTTTTGTCTGTGCCTTTCCTGACGGGAAATAT 1 84 0 CTTGTCCTGA 0.881813 -37 CTTTTGCGCCCTAAGAATTTGCTCATTTTGCTCCC 2 206 1 CTAGTGCTCA 0.986329 -44 ACCATTTTAGCTTTGAGGGGGATCACCGCCAACTA 5 83 0 CTTGGGATCA 0.871967 -98 CAATTTTTATCTGTGATCTAGATCACCTCCCATGG 6 85 0 CTTGTGATCA 0.970288 -216 GTTAAAATTCTTCGACATAGCTGATAAATCCCGG 7 124 0 CTCGTGCTGA 0.971874 -24 AAAAAATTAGTTCAGGGGTTGATCAATGACGTTTT 8 84 0 TTAGTGATCA 0.640094 -107 GCTAGTATCACTGAGCAGTTGCCCATTCCCACCAT 9 21 1 CTAGTGCCCA 0.937679 -264 CCGCCCTTTGCTCCGGCCTATCTGAATTTACGACA 9 132 1 CTCGTTCTGA 0.772081 -153 GTCTTGCCCTCTCTGTTTTAGCTCAGTACATTTAA 10 15 1 CTTGTGCTCA 0.991434 -255 AGAGTTTTTGCTATGATGTCGCTCAATTACAGGTG 10 187 1 CTTGTGCTCA 0.991434 -83 ** ** * ***** Masking position 2 Map Score: 5.38064 Number of sites scoring better than the average of aligned sites = 196 Number in coding regions = 164 Number in noncoding regions = 32 Number of orfs with sites within 600 bp upstream = 34 Fraction of orfs with sites within 600 bp upstream = 0.00546097 Motif number 8 GTGAGCTAATAACCATGCGCCTAGTCGCTTT 3 25 1 AACCTGCGCC 0.954642 -138 TGCCCAATCAAGCGGCAAAAAATCTT 5 6 1 AATCAGCGGC 0.960617 -175 TTGGGAACTAGACCGATCGCCAATCCGTTTA 7 80 1 GACCATCGCC 0.956618 -68 CTGTCAGCAAAATCGACAGCCAAAGGCCAGC 8 29 1 AATCACAGCC 0.873587 -162 CGAACTAGCGGCTAGTATCACT 9 2 1 GAACAGCGGC 0.959853 -283 TTTGACAGTCAAACAACCGCCCTTTGCTCCG 9 116 1 AAACACCGCC 0.967962 -169 GCATAGACAAAACCCAGAGGCCACGGTAAAC 10 148 0 AACCAGAGGC 0.942892 -122 TAATTTTCCGGACCAACCGCC 11 61 1 GACCACCGCC 0.988715 -11 **** ****** Masking position 2 Map Score: 4.59262 Number of sites scoring better than the average of aligned sites = 355 Number in coding regions = 329 Number in noncoding regions = 26 Number of orfs with sites within 600 bp upstream = 32 Fraction of orfs with sites within 600 bp upstream = 0.00513974 Motif number 9 TTCGAGCCATGGACTCCTCCCACCCAAAAC 1 15 1 GGACTCCTCC 0.939382 -106 CGTCCCCAAACGATCCCGCCTTCCCAGGGT 3 140 0 CGATCCCGCC 0.922277 -23 TTTTCTGTATCTATTTCTCCTCCAATGTAA 6 42 1 CTATTTCTCC 0.868659 -259 TTCTAACTCAGTTTTCCTCCGCCATATTTT 6 146 1 GTTTTCCTCC 0.927602 -155 TGATCAATGACGTTTTCTCCATCAGACTAG 8 70 0 CGTTTTCTCC 0.961333 -121 GCAAAATCTTCGTCCCTTCCCAGTTGGAAA 8 132 1 CGTCCCTTCC 0.857604 -59 ATCTTTTTCCCTTTCTCTCCGTCGCTGTTT 10 122 1 CTTTCTCTCC 0.870484 -148 TATCTTTGACCGTTTCTTCCTGTCCCGCTA 11 33 1 CGTTTCTTCC 0.937256 -39 GGGTGGGTCAGGATTCCTCCGTTATGCGGG 12 66 1 GGATTCCTCC 0.969396 -119 ********** Masking position 9 Map Score: 4.51523 Number of sites scoring better than the average of aligned sites = 655 Number in coding regions = 596 Number in noncoding regions = 59 Number of orfs with sites within 600 bp upstream = 71 Fraction of orfs with sites within 600 bp upstream = 0.0114038 Motif number 10 TGCTTGGGCGAGGGGATCTCTGTCGTCGTAAAAA 2 125 1 AGGGGATCGC 0.985172 -125 CCGAAGCAGCAGGGTATTGAAGCCTTTTTACGAC 2 149 0 AGGGTATTGC 0.917239 -101 TTAGCTTTGAGGGGGATCACCGCCAACTAACCAA 5 78 0 GGGGGATCGC 0.992378 -103 CACCCATGGGAGGTGATCTAGATCACAGATAAAA 6 82 1 AGGTGATCAC 0.887596 -219 TTAGTTCAGGGGTTGATCAATGACGTTTTCTCCA 8 79 0 GGTTGATCGC 0.953547 -112 GTACATTTAAGGATTATTAATGACACCAATCATA 10 40 1 GGATTATTGC 0.77397 -230 GTGAACCGGCAGGTGATTGTAGACGTTAACATTT 10 224 1 AGGTGATTGC 0.963502 -46 AACTAACCTAGGGTTATCTTTGACCGTTTCTTCC 11 19 1 GGGTTATCGC 0.987737 -53 TTTTTTTGTGGGCTTATCAAAGGCTTGTGTAGTT 12 140 1 GGCTTATCGC 0.939329 -45 ******** * * Masking position 7 Map Score: 5.12787 Number of sites scoring better than the average of aligned sites = 633 Number in coding regions = 578 Number in noncoding regions = 55 Number of orfs with sites within 600 bp upstream = 68 Fraction of orfs with sites within 600 bp upstream = 0.0109219 Motif number 11 CACAGGGAGAGCAACGCTTCCGAAGCAGCA 2 172 0 GCAACGCTTC 0.922133 -78 AGATAACAGTGGGACGCACCAAGGCAATTA 4 59 1 GGGACGCACC 0.846962 -120 GGGCAAAAAGGGAACGCCCCTGGCATCGAT 5 123 0 GGAACGCCCC 0.674719 -58 CGCTGTCAATGGGTCGCCCCGTATAATAAC 7 45 1 GGGTCGCCCC 0.588884 -103 TGGCATTGGGGCAGGGCCCCAGTGTGACGG 9 56 1 GCAGGGCCCC 0.918964 -229 AGCAACGCTCCTCACAAGCCA 10 259 0 GCAACGCTCC 0.950965 -11 ********** Masking position 6 Map Score: 2.95902 Number of sites scoring better than the average of aligned sites = 220 Number in coding regions = 204 Number in noncoding regions = 16 Number of orfs with sites within 600 bp upstream = 23 Fraction of orfs with sites within 600 bp upstream = 0.00369419 Motif number 12 AAATATGCTTGGTAAAGGGAGATGAATTGG 1 60 0 GGTAAAGGGA 0.929794 -61 ATTCTCCCGTGATAGAGGGAAAAAGCTCAG 2 34 1 GATAGAGGGA 0.972739 -216 GTGTAGGGACGATAGACCGCTGGGATTTGT 2 96 1 GATAGACCGC 0.858864 -154 GGCTATGCGAGATAAACCGATCTAAGCTTA 4 141 1 GATAAACCGA 0.959767 -38 TCTTTCCGAGGATATACGGATAAATAATAT 6 262 1 GATATACGGA 0.945004 -39 TTCTATGAAAGATAAACGGATTGGCGATCG 7 93 0 GATAAACGGA 0.982619 -55 CAGCGACGGAGAGAAAGGGAAAAAGATTCC 10 119 0 GAGAAAGGGA 0.929794 -151 ********** Masking position 6 Map Score: 3.3844 Number of sites scoring better than the average of aligned sites = 45 Number in coding regions = 38 Number in noncoding regions = 7 Number of orfs with sites within 600 bp upstream = 8 Fraction of orfs with sites within 600 bp upstream = 0.00128493 Motif number 13 ACTGACAGCTTAAACTTTTGTGATAGGCTATG 4 116 1 TAACTTTGTG 0.788276 -63 ATGGTTTGACTTTACTGTTAAAAACTA 5 164 0 TGACTTTCTG 0.977874 -17 ATGTTTTTACTGGTCATTACTGGCTTCCGATT 6 11 1 TGTCATTCTG 0.945075 -290 TGCAAATGACTTAACATTCCTGAGTCAATTCT 6 118 1 TAACATTCTG 0.965174 -183 GTTAAACTATCCTGACCAAAGGGA 7 3 1 TAACTATCTG 0.780035 -145 TTAAAATTCTTCGACATAGCTGATAAATCCCG 7 125 0 TGACATACTG 0.895656 -23 CCTTTGGCTGTCGATTTTGCTGACAGATTTAA 8 23 0 TGATTTTCTG 0.812481 -168 TCGGTTATGGTCATTGCTGGAGCCTGGGC 9 266 0 TGTCATTCTG 0.945075 -19 ATTGTAGACGTTAACATTTATGGCTTGTGAGG 10 239 1 TAACATTATG 0.755791 -31 * ****** *** Masking position 11 Map Score: 2.93851 Number of sites scoring better than the average of aligned sites = 264 Number in coding regions = 229 Number in noncoding regions = 35 Number of orfs with sites within 600 bp upstream = 32 Fraction of orfs with sites within 600 bp upstream = 0.00513974 Motif number 14 ATAATAACTTGGGTTGGGAACTAGACCGATC 7 67 1 GGGTTGGGAC 0.977045 -81 AAAGATAAACGGATTGGCGATCGGTCTAGTT 7 85 0 GGATTGGCAT 0.96429 -63 CGAAGATTTTGCGTTGGCCATAGTCAAAAAA 8 113 0 GCGTTGGCAT 0.94825 -78 TTTCAAAGGGGGGGTGGGAATCTTTTTCCCT 10 103 1 GGGGTGGGAT 0.977045 -167 GTTATGCGGGGGATTGGACATTTTGATTTGT 12 86 1 GGATTGGAAT 0.908258 -99 CTCCGCCAGCGGGTGGGGTATTTTTTTTGTG 12 119 1 GGGTGGGGAT 0.977045 -66 ******** ** Masking position 10 Map Score: 0.646123 Number of sites scoring better than the average of aligned sites = 357 Number in coding regions = 325 Number in noncoding regions = 32 Number of orfs with sites within 600 bp upstream = 37 Fraction of orfs with sites within 600 bp upstream = 0.00594282 Motif number 15 CAAAGCTAAAATGGTGCGACATCGATGCCAGG 5 103 1 ATGGTGACAT 0.985743 -78 ATGGTTTGACTTTACTGTTAAA 5 169 0 ATGGTGACTT 0.947755 -12 TGACTCAGGAATGTTAAGTCATTTGCAATTTT 6 113 0 ATGTTGTCAT 0.829107 -188 GTTAAAATTCTTCGACATAGCTGATAAA 7 130 0 ATTCTGACAT 0.821491 -18 TGTTTCCGTAATGGTATGTCGTAAATTCAGAT 9 151 0 ATGGTGTCGT 0.921281 -134 CGCACCTGTAATTGAGCGACATCATAGCAAAA 10 192 0 ATTGAGACAT 0.806459 -78 CCACCCAAAGATGGTTAGACATTTTCAATTAA 12 40 0 ATGGTGACAT 0.985743 -145 ***** ***** Masking position 1 Map Score: 0.485754 Number of sites scoring better than the average of aligned sites = 65 Number in coding regions = 56 Number in noncoding regions = 9 Number of orfs with sites within 600 bp upstream = 8 Fraction of orfs with sites within 600 bp upstream = 0.00128493