AlignACE version 2.2  July 7, 1998
/home/amcguire/bin/alignACE -i056_Branched_Chain_Aminoacid_biosynthesis__mjan_reg_100.orf -o056_mjan_100.ace -a/home/amcguire/genomes/ORF_mjan.txt -z/home/amcguire/genomes/mjan.fna -g0.31 -x5 -e 
Parameter values:
 expect =  	5
 ncols =   	10
 npass =   	1000
 maxnpass =	100
 nruns =   	1000
 maxnruns =	100
 repeat =  	15
 maxreps = 	3
 nread =   	500
 ncycles = 	1
 fragment =	1
 psfact =  	0.1
 gcback =  	0.31
 maxlen =  	30
 weight =  	0.8
 exclude = 	1

Input sequences:
#1	RMJ04589	300	M_jannaschii_chromosome
#2	RMJ13440	183	M_jannaschii_chromosome
#3	RMJ06792	300	M_jannaschii_chromosome
#4	RMJ13997	135	M_jannaschii_chromosome
#5	RMJ05932	226	M_jannaschii_chromosome
#6	RMJ04294	123	M_jannaschii_chromosome
#7	RMJ00596	205	M_jannaschii_chromosome
#8	RMJ11884	235	M_jannaschii_chromosome

Motif number 1

TACGAAAAACTATGAAAATTTTCTTTTAATC	1	31	0	TATGAAAATT	    0.915248	-270
TAAATTTAGTTATGAAAAATTGAACACCCTC	1	69	1	TATGAAAATT	    0.915248	-232
TGTCATATCCTATCAAAAATATGCAAATCAA	1	195	1	TATCAAAATA	    0.927726	-106
AAAATATGCAAATCAAAAATTTCAGGTATCA	1	210	1	AATCAAAATT	    0.893925	-91
TAAATCTTACTATAAAAAGGATTG       	1	287	1	TATAAAAAGA	    0.912903	-14
AAAGACTAAAAATAAAAAATAGAATTAAAGA	2	17	0	AATAAAAATA	    0.836186	-167
AAAAGTTTGATATAAAAAAGTGTTGGTATTT	2	68	0	TATAAAAAGT	    0.945164	-116
TTTGAAGAACTTTAAAAAGTTTGATATAAAA	2	82	0	TTTAAAAATT	    0.923087	-102
AAATCCTTAGTATAAAAATTTTTATATATGA	2	117	1	TATAAAAATT	    0.954598	-67
GATGATGAGCAATAAAAAGCTGACTTAATAT	3	23	0	AATAAAAACT	    0.800404	-278
TAAAAAAGTTTATAAAAAATCTTTCATGAAT	3	107	0	TATAAAAATC	    0.727254	-194
GTGTAAGTGATATAAAAAAGTTTATAAAAAA	3	119	0	TATAAAAAGT	    0.945164	-182
GTATAGTGCCTTTCAAAACTTATTGAGATAA	3	157	1	TTTCAAAATT	    0.923367	-144
ATATAGTGCCTTTCAAAATTAATAAACTTAT	3	238	0	TTTCAAAATA	     0.87991	-63
TAACTTAACCTTTCAAAATGATAGATTAAAG	4	28	0	TTTCAAAAGA	    0.857277	-108
GATGGGATGTTATAAAAATTTTACCTAATCA	4	79	0	TATAAAAATT	    0.954598	-57
ATAACAGAAAATTAAAAAGTTTTGGTGGGAT	4	114	1	ATTAAAAATT	    0.827333	-22
AATTCTGGAATTTCAAAAGTTAAAGTTATCG	5	45	0	TTTCAAAATT	    0.923367	-182
TGTTGAAAAAATTGAAAAAGAATGTGAAGAA	5	171	1	ATTGAAAAGA	    0.550933	-56
TATTGTTTAATATGAAAATCTAAATATAAGA	6	25	0	TATGAAAACT	    0.837638	-99
TTATAACTAAAATAAAAAGTAAGTGAATAAA	6	72	0	AATAAAAATA	    0.836186	-52
CATTAATCATATTCAAAAGGTGAAATA    	6	107	1	ATTCAAAAGT	    0.797715	-17
AAATCGATACTATAAAAACCATATAATAATA	7	146	0	TATAAAAACA	    0.859315	-60
ATATAGTGTCTTTCAAAAACATTTTGAAGTA	8	184	0	TTTCAAAACA	    0.777796	-52
AAAGACACTATATAAAAAAGTTTTTGGTGCT	8	202	1	TATAAAAAGT	    0.945164	-34
          ******** **

Masking position 3
Map Score:   35.9742

Number of sites scoring better than the average of aligned sites = 1199
Number in coding regions = 815
Number in noncoding regions = 384
Number of orfs with sites within 600 bp upstream = 349
Fraction of orfs with sites within 600 bp upstream = 0.0560553


Motif number 2

AAAAACTATGAAAATTTTCTTTTAATCTTA	1	28	0	AAAATTTTCT	     0.57679	-273
TTATGATACCTGAAATTTTTGATTTGCATA	1	214	0	TGAAATTTTT	    0.543304	-87
ATTAAATACCAACACTTTTTTATATCAAAC	2	65	1	AACACTTTTT	    0.852034	-119
TTTTTTATATCAAACTTTTTAAAGTTCTTC	2	80	1	CAAACTTTTT	    0.782196	-104
TCCTTAGTATAAAAATTTTTATATATGATT	2	120	1	AAAAATTTTT	    0.489413	-64
CTTTACCCTTAACATTTTTTGGTGATTGG 	2	165	1	AACATTTTTT	    0.808684	-19
TTATTCATGAAAGATTTTTTATAAACTTTT	3	105	1	AAGATTTTTT	    0.808684	-196
AGATTTTTTATAAACTTTTTTATATCACTT	3	116	1	TAAACTTTTT	    0.938184	-185
AGTGCCTTTCAAAACTTATTGAGATAATAA	3	161	1	AAAACTTATT	    0.930236	-140
TCAAAATTAATAAACTTATTATTAAACTTT	3	227	0	TAAACTTATT	    0.909303	-74
TCTGATTAGGTAAAATTTTTATAACATCCC	4	77	1	TAAAATTTTT	    0.722032	-59
 AATCCCACCAAAACTTTTTAATTTTCTGT	4	117	0	AAAACTTTTT	    0.896341	-19
TTGAAATTCCAGAATTTATTGATAAAAATA	5	60	1	AGAATTTATT	    0.817173	-167
AGCTCCTTTTTGAATTTATTTTTATCAATA	5	76	0	TGAATTTATT	    0.728481	-151
TTTCTCTCCCAAGATTTTTTTCTTGATGAA	5	113	0	AAGATTTTTT	    0.808684	-114
TTTCTGAATTTGAATTTTTTTCTAAATTCT	5	198	0	TGAATTTTTT	    0.804562	-29
TACAGAAAACAAAACTTATTTATTCACTTA	6	54	1	AAAACTTATT	    0.930236	-70
ATTTATGTTTTAAATTTATTTCACTATAAT	7	82	1	TAAATTTATT	    0.882638	-124
ATTAATACGGTATATTTATTGTTTCTATTT	8	39	0	TATATTTATT	    0.605691	-197
TTTATTGTCAAATATTTATTAATAAACTTT	8	111	0	AATATTTATT	    0.696147	-125
AAGGCATTTAAATATTTATTGTCAAATATT	8	125	0	AATATTTATT	    0.696147	-111
GTGTCTTTCAAAAACATTTTGAAGTAACCA	8	180	0	AAAACATTTT	    0.511765	-56
GACAGCACCAAAAACTTTTTTATATAGTGT	8	206	0	AAAACTTTTT	    0.896341	-30
          **********

Masking position 7
Map Score:   25.377

Number of sites scoring better than the average of aligned sites = 1021
Number in coding regions = 697
Number in noncoding regions = 324
Number of orfs with sites within 600 bp upstream = 261
Fraction of orfs with sites within 600 bp upstream = 0.041921


Motif number 3

AAAATTAAGTTAAGATTAAAAGAAAATTTTCATAGTT	1	18	1	TATTAAAAAT	     0.65956	-283
AAAATTTTCATAGTTTTTCGTAAAGTTGTTAAATTTA	1	40	1	TATTTGTATT	    0.649096	-261
AGCCTTGTCATATCCTATCAAAAATATGCAAATCAAA	1	190	1	TATATAAAAT	    0.659508	-111
CTTTTTATAGTAAGATTTAAAAATAATAAAATAGATA	1	269	0	TATTTAAAAT	    0.951312	-32
ACTAAGGATTTAGCATTTGAAGAACTTTAAAAAGTTT	2	91	0	TATTTAAATT	    0.947565	-93
AATTTTTATATATGATTTCAATTTTATCATTACTTTA	2	133	1	TATTTAATAT	    0.772362	-51
ACTTTACCCTTAACATTTTTTGGTGATTGG       	2	164	1	TATTTTTGAT	    0.525811	-20
ATTATCTCAATAAGTTTTGAAAGGCACTATACTTTTA	3	152	0	TATTTAAGAC	    0.661035	-149
AACTTATTATTAAACTTTGAACGCCTTCCTTTAGGAG	3	208	0	TATTTAAGTT	    0.956366	-93
CGCTATAACTTAACCTTTCAAAATGATAGATTAAAGT	4	27	0	TATTTAAAAT	    0.951149	-109
GGTTAAGTTATAGCGTTTTAATGATATATCTGATTAG	4	49	1	TATTTAAGAT	    0.960661	-87
TCTGATTAGGTAAAATTTTTATAACATCCCATCATAA	4	77	1	TATTTTAAAT	    0.689782	-59
       TTTTATTTTTTGGTGGTAATTATGGCTACA	5	4	1	TATTTGTGAT	    0.714157	-223
GGAAAAGGTGTAGATGTTGAAAAAATTGAAAAAGAAT	5	157	1	TAGTTAAATT	    0.583452	-70
     TATTTCACCTTTTGAATATGATTAATGTAGAT	6	102	0	CATTTAAAAT	    0.619537	-22
AACCAACATTTATGTTTTAAATTTATTTCACTATAAT	7	75	1	TATTTAATTT	    0.758214	-131
TTTATTTCACTATAATTTAGTTGTGTTCTAATGGTAT	7	96	1	TATTTGTGTT	     0.69795	-110
AATAATAACATAGAATTTAAATACCATTAGAACACAA	7	116	0	TATTTAAAAT	    0.951338	-90
AATTCTATGTTATTATTATATGGTTTTTATAGTATCG	7	136	1	TATTAATGTT	    0.471896	-70
        AATACTATTTTAAGGTTTTCGGTTTAATT	7	187	0	TATTTAAGTT	    0.957573	-19
TCCCTCTTATTAACGTTTAATAGAAATAGAAACAATA	8	16	1	TATTTATGAT	    0.906971	-220
TTGAAGACAGTATAGTTTAAAGATATTTTTAAGTATT	8	66	0	TATTTAAATT	    0.947588	-170
TATTTATTAATAAACTTTAATTAATTTTGAAGACAGT	8	92	0	TATTTATATT	    0.878307	-144
ATTTGACAATAAATATTTAAATGCCTTCCTTTGGAAG	8	128	1	AATTTAAGTT	    0.558081	-108
AAGTAACCAGTAGCTTATGAACACCTTCCAAAGGAAG	8	152	0	TATATAAATT	    0.641545	-84
          **   *** ** *  **

Masking position 2
Map Score:   25.54

Number of sites scoring better than the average of aligned sites = 1172
Number in coding regions = 901
Number in noncoding regions = 271
Number of orfs with sites within 600 bp upstream = 248
Fraction of orfs with sites within 600 bp upstream = 0.039833


Motif number 4

         GAAACCAAAAATTAAGTTAAGATTA	1	2	1	AAAAAATTAA	    0.968815	-299
TTCTAAAGATAAAGGACATAATAAATGTCTTTCG	1	103	0	AAAAAAATAA	    0.922113	-198
ATATTTGCAAAAACCAATAATTTAGATGCCAATT	1	136	1	AAAATATTTA	    0.836248	-165
ATTAATTAACAAAAGAAAAATTAAGCCTTGTCAT	1	167	1	AAAAAATTAA	    0.968815	-134
TAAAAATAATAAAATAGATATTTAGTAGAACTCA	1	255	0	AAAAAATTTA	    0.914907	-46
GACTAAAAATAAAAAATAGAATTAAAGAAAGAAA	2	11	0	AAAAAAATTA	     0.80563	-173
TTAAGATTAGTAATTAAATATTAAATACCAACAC	2	46	1	TAAAAATTAA	     0.83821	-138
GATAAAATTGAAATCATATATAAAAATTTTTATA	2	127	0	AAAAAATAAA	    0.915806	-57
TGTTAAGGGTAAAGTAATGATAAAATTGAAATCA	2	145	0	AAAATATAAA	    0.836249	-39
GTTATTCTTATAAAGACTAATTAAATGGTGAGAT	3	276	1	TAAATATTAA	    0.716829	-25
TAACCTTTCAAAATGATAGATTAAAGTTCATTTA	4	20	0	AAAAAATTAA	    0.968815	-116
GTAGATGTTGAAAAAATTGAAAAAGAATGTGAAG	5	166	1	AAAATAAAAA	    0.660569	-61
GATTATAACTAAAATAAAAAGTAAGTGAATAAAT	6	71	0	AAAAAAGTAA	    0.842099	-53
GATACTATAAAAACCATATAATAATAACATAGAA	7	138	0	AAAAAAATAA	     0.92211	-68
ATAGAAATAGAAACAATAAATATACCGTATTAAT	8	35	1	AAAAAATATA	     0.79203	-201
TATTTATTGTCAAATATTTATTAATAAACTTTAA	8	109	0	CAAATATTAA	    0.679813	-127
          ***  * * *****

Masking position 6
Map Score:   17.3074

Number of sites scoring better than the average of aligned sites = 849
Number in coding regions = 575
Number in noncoding regions = 274
Number of orfs with sites within 600 bp upstream = 211
Fraction of orfs with sites within 600 bp upstream = 0.0338901


Motif number 5

AATTAATTGGCATCTAAATTATTGGTTTTT	1	144	0	CATCTAAATT	    0.778758	-157
ATTTAGATGCCAATTAATTAACAAAAGAAA	1	155	1	CAATTAATTA	    0.874773	-146
ATGGTGAGTTCTACTAAATATCTATTTTAT	1	251	1	CTACTAAATA	    0.812296	-50
TAGTAATTAAATATTAAATACCAACACTTT	2	53	1	ATATTAAATA	    0.707788	-131
  AATCTCACCATTTAATTAGTCTTTATAA	3	283	0	CATTTAATTA	    0.846313	-18
TTAATATGAAAATCTAAATATAAGAAAATA	6	20	0	AATCTAAATA	    0.882402	-104
CATTATCCTAAAATTAAATAGTATGGTGAA	7	15	0	AAATTAAATA	    0.868183	-191
CATTAGAACACAACTAAATTATAGTGAAAT	7	99	0	CAACTAAATT	     0.80988	-107
AATAACATAGAATTTAAATACCATTAGAAC	7	120	0	AATTTAAATA	    0.806866	-86
ACTGTCTTCAAAATTAATTAAAGTTTATTA	8	92	1	AAATTAATTA	    0.756214	-144
CAAAGGAAGGCATTTAAATATTTATTGTCA	8	131	0	CATTTAAATA	    0.925504	-105
          **********

Masking position 6
Map Score:   6.82436

Number of sites scoring better than the average of aligned sites = 483
Number in coding regions = 340
Number in noncoding regions = 143
Number of orfs with sites within 600 bp upstream = 143
Fraction of orfs with sites within 600 bp upstream = 0.0229682


Motif number 6

TTCATAACTAAATTTAACAACTTTACGAAA	1	55	0	AATTTAACAA	    0.711807	-246
TAAAAAATAGAATTAAAGAAAGAAA     	2	6	0	AATTAAAGAA	    0.751036	-178
CCTAAATTATTATTCATGAAAGATTTTTTA	3	96	1	TATTCATGAA	    0.836496	-205
TAATAAAAGGTATTAATGAACGCCTCCTAA	3	185	1	TATTAATGAA	    0.762365	-116
TGCCTTTCAAAATTAATAAACTTATTATTA	3	233	0	AATTAATAAA	    0.818373	-68
AAGTTCATTTAATTCATCAA          	4	1	0	AATTCATCAA	    0.886167	-135
GATAAAAATAAATTCAAAAAGGAGCTTGAA	5	80	1	AATTCAAAAA	    0.853635	-147
GAATGTGAAGAATTTAGAAAAAAATTCAAA	5	190	1	AATTTAGAAA	    0.783363	-37
AAAAAATTCAAATTCAGAAATTGAATTTT 	5	208	1	AATTCAGAAA	    0.902447	-19
TAGATTTTCATATTAAACAATACAGAAAAC	6	34	1	TATTAAACAA	    0.773769	-90
TTATAAACGATATTTAGCAATTACACGGAG	7	47	0	TATTTAGCAA	    0.771779	-159
TTTAATTTGATATTCAGAAATCGATACTAT	7	164	0	TATTCAGAAA	    0.888707	-42
AATTAAAGTTTATTAATAAATATTTGACAA	8	107	1	TATTAATAAA	    0.795476	-129
          **********

Masking position 6
Map Score:   8.52154

Number of sites scoring better than the average of aligned sites = 574
Number in coding regions = 470
Number in noncoding regions = 104
Number of orfs with sites within 600 bp upstream = 90
Fraction of orfs with sites within 600 bp upstream = 0.0144555


Motif number 7

AAACTTTTTAAAGTTCTTCAAATGCTAAAT	2	91	1	AAGTTCTTCA	    0.946317	-93
ATATGATTTCAATTTTATCATTACTTTACC	2	142	1	AATTTTATCA	     0.87287	-42
       GAAAGGTTCATCATATTAAGTCA	3	4	1	AGGTTCATCA	    0.943619	-297
AGCCCCCGTAAGGTTCATCATCCTAAATTA	3	75	1	AGGTTCATCA	    0.943619	-226
          AATCTCACCATTTAATTAGT	3	291	0	AATCTCACCA	    0.780776	-10
AAAGTTCATTTAATTCATCAA         	4	2	0	TAATTCATCA	    0.708547	-134
GGAGCTTGAAAATTTCATCAAGAAAAAAAT	5	100	1	AATTTCATCA	     0.97544	-127
ATTCTTTTTCAATTTTTTCAACATCTACAC	5	164	0	AATTTTTTCA	    0.756628	-63
ATTTTTTTCTAAATTCTTCACATTCTTTTT	5	185	0	AAATTCTTCA	    0.886668	-42
         AAATTTCACCATACTATTTAA	7	2	1	AATTTCACCA	    0.944684	-204
          **********

Masking position 5
Map Score:   7.51613

Number of sites scoring better than the average of aligned sites = 473
Number in coding regions = 418
Number in noncoding regions = 55
Number of orfs with sites within 600 bp upstream = 45
Fraction of orfs with sites within 600 bp upstream = 0.00722775


Motif number 8

TCTTTCGATAGAGGGTGTTCAATTTTTCAT	1	80	0	GAGGGTGTTC	    0.977204	-221
CCTTCCTTTAGGAGGCGTTCATTAATACCT	3	192	0	GGAGGCGTTC	    0.969678	-109
CCTCCTAAAGGAAGGCGTTCAAAGTTTAAT	3	207	1	GAAGGCGTTC	    0.784475	-94
CCTTCCAAAGGAAGGCATTTAAATATTTAT	8	136	0	GAAGGCATTT	    0.879074	-100
CCTTCCTTTGGAAGGTGTTCATAAGCTACT	8	151	1	GAAGGTGTTC	    0.976948	-85
          **********

Masking position 8
Map Score:   4.82928

Number of sites scoring better than the average of aligned sites = 44
Number in coding regions = 8
Number in noncoding regions = 36
Number of orfs with sites within 600 bp upstream = 33
Fraction of orfs with sites within 600 bp upstream = 0.00530035


Motif number 9

TATTTAGTAGAACTCACCATATAATAATTAT	1	240	0	AACCACCATA	    0.924839	-61
TAAGTTTTGAAAGGCACTATACTTTTAGAGT	3	148	0	AAGCACTATA	    0.987098	-153
TTAATTTTGAAAGGCACTATATATCTACAGT	3	247	1	AAGCACTATA	    0.987098	-54
ATATATCATTAAAACGCTATAACTTAACCTT	4	47	0	AAACGCTATA	    0.795672	-89
        AAATTTCACCATACTATTTAATT	7	3	1	ATTCACCATA	    0.842752	-203
TTTTAAATTTATTTCACTATAATTTAGTTGT	7	89	1	ATTCACTATA	    0.904438	-117
ATTAATTTTGAAGACAGTATAGTTTAAAGAT	8	79	0	AAGCAGTATA	    0.926801	-157
ATGTTTTTGAAAGACACTATATAAAAAAGTT	8	193	1	AAGCACTATA	    0.987098	-43
          *** *******

Masking position 9
Map Score:   6.10297

Number of sites scoring better than the average of aligned sites = 70
Number in coding regions = 41
Number in noncoding regions = 29
Number of orfs with sites within 600 bp upstream = 32
Fraction of orfs with sites within 600 bp upstream = 0.00513974


Motif number 10

TTGCAAATATTCTAAAGATAAAGGACATAA	1	116	0	TCTAAAGATA	    0.931176	-185
TATAGTAAGATTTAAAAATAATAAAATAGA	1	271	0	TTTAAAAATA	    0.882559	-30
AATCTTAAAGACTAAAAATAAAAAATAGAA	2	24	0	ACTAAAAATA	    0.538862	-160
TCACTTACACTCTAAAAGTATAGTGCCTTT	3	140	1	TCTAAAAGTA	    0.777604	-161
GCGTTCAAAGTTTAATAATAAGTTTATTAA	3	221	1	TTTAATAATA	    0.882418	-80
GTTATAGCGTTTTAATGATATATCTGATTA	4	55	1	TTTAATGATA	    0.909632	-81
AATTCCAGAATTTATTGATAAAAATAAATT	5	64	1	TTTATTGATA	    0.693151	-163
AATATGAAAATCTAAATATAAGAAAATAGA	6	18	0	TCTAAATATA	    0.675615	-106
TTAGTTGTGTTCTAATGGTATTTAAATTCT	7	112	1	TCTAATGGTA	    0.824318	-94
ATAAAAACCATATAATAATAACATAGAATT	7	136	0	TATAATAATA	    0.604622	-70
GACAGTATAGTTTAAAGATATTTTTAAGTA	8	68	0	TTTAAAGATA	    0.909777	-168
TTGTCAAATATTTATTAATAAACTTTAATT	8	107	0	TTTATTAATA	    0.627349	-129
          **********

Masking position 4
Map Score:   7.68993

Number of sites scoring better than the average of aligned sites = 476
Number in coding regions = 373
Number in noncoding regions = 103
Number of orfs with sites within 600 bp upstream = 101
Fraction of orfs with sites within 600 bp upstream = 0.0162223


Motif number 11

GATTTGCATATTTTTGATAGGATATGACAA	1	194	0	TTTTTGATAG	    0.845889	-107
CCCTTAACATTTTTTGGTGATTGG      	2	170	1	TTTTTGGTGA	    0.952653	-14
TTTTCTGTTATTTATGATGGGATGTTATAA	4	95	0	TTTATGATGG	    0.897468	-41
AAATTAAAAAGTTTTGGTGGGATT      	4	122	1	GTTTTGGTGG	    0.974615	-14
    TTTTATTTTTTGGTGGTAATTATGGC	5	7	1	TTTTTGGTGG	    0.990566	-220
TATAAAAAAGTTTTTGGTGCTGTCTATGAT	8	212	1	TTTTTGGTGC	    0.967555	-24
          **********

Masking position 5
Map Score:   4.07693

Number of sites scoring better than the average of aligned sites = 152
Number in coding regions = 107
Number in noncoding regions = 45
Number of orfs with sites within 600 bp upstream = 56
Fraction of orfs with sites within 600 bp upstream = 0.00899454


Motif number 12

          **********

No masking
Map Score:   9.54559e-13

Number of sites scoring better than the average of aligned sites = 0
Number in coding regions = 0
Number in noncoding regions = 0
Number of orfs with sites within 600 bp upstream = 0
Fraction of orfs with sites within 600 bp upstream = 0


Motif number 13

          **********

No masking
Map Score:   9.54559e-13

Number of sites scoring better than the average of aligned sites = 0
Number in coding regions = 0
Number in noncoding regions = 0
Number of orfs with sites within 600 bp upstream = 0
Fraction of orfs with sites within 600 bp upstream = 0


Motif number 14

          **********

No masking
Map Score:   9.54559e-13

Number of sites scoring better than the average of aligned sites = 0
Number in coding regions = 0
Number in noncoding regions = 0
Number of orfs with sites within 600 bp upstream = 0
Fraction of orfs with sites within 600 bp upstream = 0


