AlignACE version 2.2 July 7, 1998 /home/amcguire/bin/alignACE -i342_aful_mthe_100.orf -o342_aful_mthe_100.ace -a/home/amcguire/alignace/lib/ORF_aful.txt -z/skink1/amcguire/genomes/aful.fna -g0.49 -x5 -e Parameter values: expect = 5 ncols = 10 npass = 1000 maxnpass = 100 nruns = 1000 maxnruns = 100 repeat = 15 maxreps = 3 nread = 500 ncycles = 1 fragment = 1 psfact = 0.1 gcback = 0.49 maxlen = 30 weight = 0.8 exclude = 1 Input sequences: #1 RTH01601 134 M.thermo #2 RTH01432 19 M.thermo Motif number 1 AAAAATACCTGATGATGGATC 1 1 0 GTGATGGATC 0.995738 -134 AGAATGAGCCGCGGTTTGTTGCTAAAAATAC 1 24 0 GGGTTTGTTG 0.980083 -111 GGATGGAGCAGCTGATGGTTGTGAGGAGAAT 1 50 0 GTGATGGTTG 0.998814 -85 AAAAATAGTTGGTGATGGATGGAGCAGCTGA 1 66 0 GTGATGGATG 0.99881 -69 * ********* Masking position 6 Map Score: 6.46468 Number of sites scoring better than the average of aligned sites = 86 Number in coding regions = 81 Number in noncoding regions = 5 Number of orfs with sites within 600 bp upstream = 4 Fraction of orfs with sites within 600 bp upstream = 0.000642467 Motif number 2 CGCGGTTTGTTGCTAAAAATACCTGATGAT 1 16 0 TGCTAAAAAT 0.993407 -119 CGGCTCATTCTCCTCACAACCATCAGCTGC 1 44 1 TCCTCACAAC 0.987684 -91 ATGGGGCTATTGCTAAAAATAGTTGGTGAT 1 81 0 TGCTAAAAAT 0.993407 -54 GATGTCACCCTAAAAACTGAAGTCCAA 1 118 0 CCCTAAAAAC 0.992739 -17 ********** Masking position 6 Map Score: 4.98741 Number of sites scoring better than the average of aligned sites = 80 Number in coding regions = 65 Number in noncoding regions = 15 Number of orfs with sites within 600 bp upstream = 15 Fraction of orfs with sites within 600 bp upstream = 0.00240925 Motif number 3 TTGTGAGGAGAATGAGCCGCGGTTTGTTGCTA 1 31 0 AATGAGCGCG 0.989104 -104 TTGGTGATGGATGGAGCAGCTGATGGTTGTGA 1 57 0 ATGGAGCGCG 0.998363 -78 CAATATTAATATGGGGCTATTGCTAAAAATAG 1 89 0 ATGGGGCATG 0.989662 -46 CCATATTAATATTGGACTTCAGTTTTTAGGGT 1 107 1 ATTGGACTCG 0.986881 -28 CAATGAGACGGGAGGCAAA 2 3 1 ATGAGACGGG 0.979699 -17 ******* ** * Masking position 1 Map Score: 2.39524 Number of sites scoring better than the average of aligned sites = 458 Number in coding regions = 429 Number in noncoding regions = 29 Number of orfs with sites within 600 bp upstream = 23 Fraction of orfs with sites within 600 bp upstream = 0.00369419 Motif number 4 TAGCAACAAACCGCGGCTCATTCTCCTCAC 1 31 1 CCGCGGCTCA 0.999134 -104 TCACAACCATCAGCTGCTCCATCCATCACC 1 57 1 CAGCTGCTCC 0.992391 -78 TTTGCCTCCCGTCTCATTG 2 4 0 TCCCGTCTCA 0.988279 -16 ********** Masking position 8 Map Score: 0.773343 Number of sites scoring better than the average of aligned sites = 104 Number in coding regions = 93 Number in noncoding regions = 11 Number of orfs with sites within 600 bp upstream = 10 Fraction of orfs with sites within 600 bp upstream = 0.00160617 Motif number 5 ********** No masking Map Score: -1.53887e-13 Number of sites scoring better than the average of aligned sites = 0 Number in coding regions = 0 Number in noncoding regions = 0 Number of orfs with sites within 600 bp upstream = 0 Fraction of orfs with sites within 600 bp upstream = 0 Motif number 6 ********** No masking Map Score: -1.53887e-13 Number of sites scoring better than the average of aligned sites = 0 Number in coding regions = 0 Number in noncoding regions = 0 Number of orfs with sites within 600 bp upstream = 0 Fraction of orfs with sites within 600 bp upstream = 0 Motif number 7 ********** No masking Map Score: -1.53887e-13 Number of sites scoring better than the average of aligned sites = 0 Number in coding regions = 0 Number in noncoding regions = 0 Number of orfs with sites within 600 bp upstream = 0 Fraction of orfs with sites within 600 bp upstream = 0