AlignACE version 2.2 July 7, 1998 /home/amcguire/bin/alignACE -i031_ecoli_mtub_300.orf -o031_ecoli_mtub_300.ace -a/home/amcguire/genomes/ORF_ecoli.txt -z/home/amcguire/genomes/ecoli.fna -g0.58 -x5 -e Parameter values: expect = 5 ncols = 10 npass = 1000 maxnpass = 100 nruns = 1000 maxnruns = 100 repeat = 15 maxreps = 3 nread = 500 ncycles = 1 fragment = 1 psfact = 0.1 gcback = 0.58 maxlen = 30 weight = 0.8 exclude = 1 Input sequences: #1 b1200 188 putative dihydroxyacetone kinase (EC 2.7.1.2) Motif number 1 AATACACGTAAAAGTTTCAATATGGAACAT 1 53 1 AAAGTTTCAA 0.94354 -136 TGCTTTAATGAATGTTCCATATTGAAACTT 1 64 0 AATGTTCCAT 0.990519 -125 CATTAAAGCATATGTTTCACTATGAAACAT 1 84 1 TATGTTTCAC 0.993376 -105 TAGCCCAAATTATGTTTCATAGTGAAACAT 1 95 0 TATGTTTCAT 0.989791 -94 GGATGATGTTCAACGACACGGCGA 1 175 0 GATGTTCAAC 0.962774 -14 ********** Masking position 5 Map Score: 5.42622 Number of sites scoring better than the average of aligned sites = 155 Number in coding regions = 125 Number in noncoding regions = 30 Number of orfs with sites within 600 bp upstream = 38 Fraction of orfs with sites within 600 bp upstream = 0.00610344 Motif number 2 TAATGAATGTTCCATATTGAAACTTTTACG 1 59 0 TCCATATTGA 0.716368 -130 CAAATTATGTTTCATAGTGAAACATATGCT 1 90 0 TTCATAGTGA 0.987729 -99 TGCCGATGAATCCATTGTGCATCAGGCAAG 1 137 0 TCCATTGTGC 0.99268 -52 ********** Masking position 4 Map Score: 1.88524 Number of sites scoring better than the average of aligned sites = 28 Number in coding regions = 23 Number in noncoding regions = 5 Number of orfs with sites within 600 bp upstream = 7 Fraction of orfs with sites within 600 bp upstream = 0.00112432 Motif number 3 ********** No masking Map Score: 6.63809e-14 Number of sites scoring better than the average of aligned sites = 0 Number in coding regions = 0 Number in noncoding regions = 0 Number of orfs with sites within 600 bp upstream = 0 Fraction of orfs with sites within 600 bp upstream = 0 Motif number 4 ********** No masking Map Score: 6.63809e-14 Number of sites scoring better than the average of aligned sites = 0 Number in coding regions = 0 Number in noncoding regions = 0 Number of orfs with sites within 600 bp upstream = 0 Fraction of orfs with sites within 600 bp upstream = 0 Motif number 5 ********** No masking Map Score: 6.63809e-14 Number of sites scoring better than the average of aligned sites = 0 Number in coding regions = 0 Number in noncoding regions = 0 Number of orfs with sites within 600 bp upstream = 0 Fraction of orfs with sites within 600 bp upstream = 0