AlignACE version 2.2 July 7, 1998 /home/amcguire/bin/alignACE -i072_ecoli_mtub_300.orf -o072_ecoli_mtub_300.ace -a/home/amcguire/genomes/ORF_ecoli.txt -z/home/amcguire/genomes/ecoli.fna -g0.58 -x5 -e Parameter values: expect = 5 ncols = 10 npass = 1000 maxnpass = 100 nruns = 1000 maxnruns = 100 repeat = 15 maxreps = 3 nread = 500 ncycles = 1 fragment = 1 psfact = 0.1 gcback = 0.58 maxlen = 30 weight = 0.8 exclude = 1 Input sequences: #1 fixA 300 probable flavoprotein subunit, carnitine metabolism #2 ydbO 284 orf, hypothetical protein #3 ydiQ 300 putative transport protein #4 atoS 214 sensor protein AtoS for response regulator AtoC #5 atoD 195 acetyl-CoA:acetoacetyl-CoA transferase alpha subunit #6 atoB 30 acetyl-CoA acetyltransferase #7 yqeF 251 putative acyltransferase #8 Rv0466 151 hypothetical protein Rv0466 #9 aceA 274 aceA #10 fadB2 81 fadB2 #11 fadA4 300 fadA4 #12 scoA 82 scoA #13 fadD35 115 fadD35 #14 fixB 38 fixB #15 fixA 230 fixA #16 fadE25 24 fadE25 Motif number 1 ATAGTGTGCGATAGCGGTGCGGGGTTTTCGA 3 220 0 AAGCGGTGCG 0.927354 -81 GTCTTCACAACACCCGGTACGCTCGGTTGG 8 10 0 CCCCGGTACG 0.960071 -142 AGAGTTAGCAAGCGTGCTGCGATTGGTGTTT 8 50 1 ACGTGCTGCG 0.908894 -102 TCACCCTTGAGTCCCGGTGCGAATCATGCCA 8 93 0 GCCCGGTGCG 0.982039 -59 CCAGCGTTGACACGCTGTGCCATGGTCGAGT 9 162 1 CCGCTGTGCC 0.982196 -113 TACCGTCGTCCAGGCGGTGTCAACGGCGCAG 9 209 0 CGGCGGTGTC 0.905663 -66 TTGCGCCGCGGAGCCCGGCACCGCC 11 5 1 GCGCGGAGCC 0.831054 -296 CGAGGCACTGCTGGCGGTGCCGGGCTCCGCG 11 17 0 CGGCGGTGCC 0.987116 -284 CCTTCAGGTTACCGTTGTGTGATGACGACCG 11 208 0 ACGTTGTGTG 0.439429 -93 TACCATTGCCCCCGCGGTGCGTCTAGGGACC 12 11 0 CCGCGGTGCG 0.997545 -72 GAAATGCTAGGAGGCTCTGCGGT 12 70 1 GGGCTCTGCG 0.75647 -13 CATCGAATAGCTCGTGCTACGTTAGTGACGA 13 52 0 CCGTGCTACG 0.811255 -64 GGTTCCTCCGCCTGGTGCCTACCGCATGT 13 97 0 CCCTGGTGCC 0.962902 -19 CCGGCGGATACTCCCGGTGCCCGGTTCGCGA 15 42 0 CCCCGGTGCC 0.991238 -189 GCAGGTTCCCACGCCTGTGCCTCGCCCACGA 15 73 0 AGCCTGTGCC 0.705744 -158 CCTGCTCTACCACCCGCTATGGTGTGAGGGC 15 99 1 CCCCGCTATG 0.524272 -132 TCGGTAAGTTCGCGTGGTGCGCCCTCACACC 15 119 0 CCGTGGTGCG 0.989432 -112 GAATAGTAATATCGCTGTGCGGTTGCAAAAC 15 148 1 ACGCTGTGCG 0.964634 -83 GTTCCGCAGTCGAGCGCTGCGGGCCGCCTTC 15 190 1 CAGCGCTGCG 0.94136 -41 * ********* Masking position 8 Map Score: 28.2106 Number of sites scoring better than the average of aligned sites = 3566 Number in coding regions = 3412 Number in noncoding regions = 154 Number of orfs with sites within 600 bp upstream = 148 Fraction of orfs with sites within 600 bp upstream = 0.0237713 Motif number 2 TTGAAAACACGGCGTAGCAAAAAGAAATTTTCAA 1 154 0 GCGTGCAAAG 0.881201 -147 CGGGGTTTTCGACGGAGCAAGCTGTTTTCCTTGC 3 198 0 GCGGGCAGCG 0.90549 -103 AGAGAACATTGCGGTAACACGCTTTTACCGCTAC 4 103 0 GGGTACAGCT 0.931995 -112 ACCCACAACGGTGTATGCAAGAGGGATAAAAA 5 174 1 GGTAGCAGAG 0.772064 -22 TGGTGGGTTAGTGGTTGCAAACCTTACGTGCCAG 7 108 1 GGGTGCAACT 0.976376 -144 GCCATTCGCAGAGTTAGCAAGCGTGCTGCGATTG 8 41 1 GGTTGCAGCT 0.985238 -111 TTCCGCCACGGCGTTGGCATGATTCGCACCGGGA 8 79 1 GGTTGCAGAT 0.967067 -73 CGGGACTCAAGGGTGAGCCTGAGGTACACGCGAG 8 108 1 GGTGGCCGAG 0.891895 -44 GTCGATCCGTGGGTTTACCTGCGGATTTGTCGTT 9 26 1 GGTTACCGCG 0.914848 -249 GTTTTTGGGCGACTTCGCAAAATTTGCAAAAAGT 9 88 1 GCTTGCAAAT 0.803976 -187 AAGTCCGCAGGCCGTTGCCGAAATTCGCAAGTGA 9 118 1 GCGTGCCAAT 0.840171 -157 GGGTGGACCAGCGTTGACACGCTGTGCCATGGTC 9 155 1 GGTTACAGCG 0.923262 -120 TGCCATGGTCGAGTTAGCACACCAGTGAAGCTGC 9 179 1 GGTTGCAACA 0.805082 -96 CGTCGGTATTGCGGTCGCCGACCTGGACGTTGCC 11 119 0 GGGTGCCACT 0.974117 -182 AGTTACCAACGAGGTAGCCAGCATGTGACGGGCG 11 164 1 GGGTGCCGCT 0.988452 -137 CCGATGCCACGGGTTAGCCTGCCTTAACA 15 6 0 GGTTGCCGCT 0.983508 -225 ATATCGCTGTGCGGTTGCAAAACGTGTGACCGAG 15 156 1 GGGTGCAAAG 0.958927 -75 * *** *** ** * Masking position 1 Map Score: 20.4981 Number of sites scoring better than the average of aligned sites = 1289 Number in coding regions = 1228 Number in noncoding regions = 61 Number of orfs with sites within 600 bp upstream = 66 Fraction of orfs with sites within 600 bp upstream = 0.0106007 Motif number 3 AACATCTGACTTTCAATATTGGTGATCCATA 1 115 1 TTTCAAATTG 0.987589 -186 AAAAAGAAATTTTCAATATTGTTTTATGGAT 1 139 0 TTTCAAATTG 0.987589 -162 TACGCCGTGTTTTCAATATTGGTGAGGAACT 1 172 1 TTTCAAATTG 0.987589 -129 TAAATCCAACTTTCAATATTGTTAAGTTCCT 1 196 0 TTTCAAATTG 0.987589 -105 CGTGTGACATTTTCAATATTGGTGATTAAAG 1 231 1 TTTCAAATTG 0.987589 -70 TAAAGTTTTATTTCAAAATTAAAGGGCGTGA 1 257 1 TTTCAAATTA 0.898845 -44 TTTACACTGAATGCAAGATTGTACGGTCATG 3 101 1 ATGCAAATTG 0.889806 -200 CGCATTTTTTTTGCAAGATTGTTGGCAAGGA 3 174 1 TTGCAAATTG 0.978289 -127 ****** **** Masking position 6 Map Score: 15.6624 Number of sites scoring better than the average of aligned sites = 37 Number in coding regions = 25 Number in noncoding regions = 12 Number of orfs with sites within 600 bp upstream = 11 Fraction of orfs with sites within 600 bp upstream = 0.00176678 Motif number 4 TTGTTTTTGCTTGCTCATGG 3 1 0 TTGCTCATGG 0.53172 -300 AGGGGCGAAGCTCCGCCTCAGGTGACCGAT 4 59 1 CTCCGCCTCA 0.92968 -156 AAGGTGATGATTTCTCGGCGGTGTATCATA 4 148 0 TTTCTCGGCG 0.742346 -67 CGATTGGTGTTTCCGCCACGGCGTTGGCAT 8 69 1 TTCCGCCACG 0.84875 -83 TTCTCCCCATTTCCTCCTCGCGTGTACCTC 8 128 0 TTCCTCCTCG 0.964817 -24 GCGCCGTTGACACCGCCTGGACGACGGTAG 9 211 1 CACCGCCTGG 0.866003 -64 GCTTTTGCGTCTGCTCGGCAGA 10 3 0 CTGCTCGGCA 0.688129 -79 AGCGCCACTTTTGCGCCGCAAAAGGGTGCT 10 30 0 TTGCGCCGCA 0.826533 -52 TGTTCCTCCTCAAATGCGCGAG 10 70 0 TTCCTCCTCA 0.836201 -12 TTGCGCCGCGGAGCCCGGCA 11 1 1 TTGCGCCGCG 0.962399 -300 CGTCGTTGATTTCCTCGTGGACCAGGATCA 11 61 1 TTCCTCGTGG 0.890977 -240 AAAGAATCTGCTTCTCCGGGTCGGTCAGAT 11 240 1 CTTCTCCGGG 0.8652 -61 TTATGACTACCTCCTCAGCGCACCCGAAAG 11 273 0 CTCCTCAGCG 0.863908 -28 TCCCTAGACGCACCGCGGGGGCAATGGTAT 12 13 1 CACCGCGGGG 0.76418 -70 GGTTCCTCCGCCTGGTGCCTACCGC 13 101 0 CTCCGCCTGG 0.978553 -15 GTTCCCACGCCTGTGCCTCGCCCACGACCG 15 70 0 CTGTGCCTCG 0.628183 -161 TATTCGGTAAGTTCGCGTGGTGCGCCCTCA 15 123 0 GTTCGCGTGG 0.529794 -108 ACTGTGGTTCGTCCTCCTCGAAGGCGGCCC 15 210 0 GTCCTCCTCG 0.921822 -21 ********** Masking position 6 Map Score: 14.3546 Number of sites scoring better than the average of aligned sites = 3504 Number in coding regions = 3332 Number in noncoding regions = 172 Number of orfs with sites within 600 bp upstream = 180 Fraction of orfs with sites within 600 bp upstream = 0.028911 Motif number 5 TCAAAATTAAAGGGCGTGATATCTGTAATTA 1 269 1 AGGGCGTGAA 0.927781 -32 CAAGATTGTACGGTCATGGAATAACTATATT 3 114 1 CGGTCATGGA 0.893651 -187 GGACGACGGTAGGGCGTCAGCGTTTTCGGCA 9 229 1 AGGGCGTCAC 0.92861 -46 CATGCCAAGGTGGTCGTGATACCACTCGATG 11 89 1 TGGTCGTGAA 0.849824 -212 GTCGGTATTGCGGTCGCCGACCTGGACGTTG 11 121 0 CGGTCGCCGC 0.953001 -180 CAGCATGTGACGGGCGTGGACTTGATCGGTC 11 182 1 CGGGCGTGGC 0.995409 -119 TGGACTTGATCGGTCGTCATCACACAACGGT 11 198 1 CGGTCGTCAC 0.977863 -103 GAGTATCCGCCGGTCGTGGGCGAGGCACAGG 15 60 1 CGGTCGTGGC 0.994097 -171 GGGCGAGGCACAGGCGTGGGAACCTGCTCTA 15 77 1 CAGGCGTGGA 0.915386 -154 ********* * Masking position 5 Map Score: 5.99155 Number of sites scoring better than the average of aligned sites = 664 Number in coding regions = 646 Number in noncoding regions = 18 Number of orfs with sites within 600 bp upstream = 25 Fraction of orfs with sites within 600 bp upstream = 0.00401542 Motif number 6 GTTTGAACTGTGACGGATTTCGCACCCTATTTTTA 2 156 0 TGAGATCGCC 0.970182 -129 ATAACTATATTGCCGCAAATTGCACTTTTGTTTTA 3 134 1 TGCGATTGCC 0.990574 -167 GGTCACCTGAGGCGGAGCTTCGCCCCTTTGAAATA 4 51 0 GGCGGTCGCC 0.948522 -164 GAGAGAACATTGCGGTAACACGCTTTTACCGCTAC 4 103 0 TGCGAACGCT 0.837072 -112 AAACTTGCTATGCAGAAATTTGCACAGTGCGCAAT 5 16 1 TGCGATTGCC 0.990574 -180 TGAGCGGCTATGCAGAAAATTGCGCACTGTGCAAA 5 34 0 TGCGATTGCC 0.990574 -162 CCGGATCCGCAGGACGTCGATCCGTGGGTTTA 9 8 1 CGCGATCGAC 0.895079 -267 CGCAGGCCGTTGCCGAAATTCGCAAGTGAAATGGG 9 123 1 TGCGATCGCA 0.97136 -152 GTGGCGCTTTTGCGTCTGCTCGCGCATTTGAGGAG 10 52 1 TGCTTTCGCC 0.836857 -30 AGCGCTCGACTGCGGAACCTCGGTCACACGTTTTG 15 173 0 TGCGATCGGC 0.981758 -58 *** * * **** * Masking position 12 Map Score: 7.50237 Number of sites scoring better than the average of aligned sites = 596 Number in coding regions = 573 Number in noncoding regions = 23 Number of orfs with sites within 600 bp upstream = 26 Fraction of orfs with sites within 600 bp upstream = 0.00417604 Motif number 7 ATTCACCCGAAACAAAAATGTGATACCAAT 1 19 0 AACAAAAATG 0.949953 -282 AACTATTAGCAACAGAAATGTGAAACATCT 2 22 0 AACAGAAATG 0.948992 -263 CGATCGCTAAAACAAAAGTGCAATTTGCGG 3 146 0 AACAAAAGTG 0.948992 -155 ACAATCTTGCAAAAAAAATGCGATCGCTAA 3 166 0 AAAAAAAATG 0.881228 -135 TCTACAAATTAAACAAAATGGAGGGCAATT 7 26 1 AAACAAAATG 0.833129 -226 TTAATTGTTTAAAAAAAGTGATTTTTATCA 7 179 0 AAAAAAAGTG 0.879116 -73 AATGATAATTAACTGAAATGCTAGGAGGCT 12 56 1 AACTGAAATG 0.821105 -27 AGTGACGATTAACCGAAGTGTCCAGCATGA 13 30 0 AACCGAAGTG 0.924649 -86 ********** Masking position 6 Map Score: 5.32007 Number of sites scoring better than the average of aligned sites = 118 Number in coding regions = 100 Number in noncoding regions = 18 Number of orfs with sites within 600 bp upstream = 22 Fraction of orfs with sites within 600 bp upstream = 0.00353357 Motif number 8 GCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTG 1 54 1 GTTAATTTAT 0.843556 -247 GAAATAAAACTTTAATCACCAATATTGAAAATGTCA 1 235 0 TTTAATCTAT 0.694631 -66 ATCGGTGGTGTTAATTACAGATATCACGCCCTTTA 1 276 0 GTTAATCTAC 0.979521 -25 TAGATAGGGCGTTAATCTCCCATACTTAACCTGGTT 7 63 0 GTTAATCTAT 0.958684 -189 GTAAATGATTGTTAATTACAGTTATCTTAATTGTTT 7 199 0 GTTAATCTAC 0.855526 -53 CGGATTTGTCGTTACTGGCGGGTAGCTTCTGAAACG 9 47 1 GTTACTCTAC 0.847071 -228 TAGCATTTCAGTTAATTATCATTAACTGAGGTGAGA 12 43 0 GTTAATTTAC 0.499147 -40 GGACACTTCGGTTAATCGTCACTAACGTAGCACGAG 13 37 1 GTTAATTTAC 0.562813 -79 TCTGACTCGCGTTAACATCGAATAGCTCGTGCTACG 13 62 0 GTTAACCTAC 0.940464 -54 ****** * ** * Masking position 4 Map Score: 5.90658 Number of sites scoring better than the average of aligned sites = 72 Number in coding regions = 58 Number in noncoding regions = 14 Number of orfs with sites within 600 bp upstream = 21 Fraction of orfs with sites within 600 bp upstream = 0.00337295 Motif number 9 GAATAGAGGGCGTTTTTTCGTTAATTTTGA 1 45 1 CGTTTTTTCG 0.841109 -256 ATTGAAAATTTCTTTTTGCTACGCCGTGTT 1 153 1 TCTTTTTGCT 0.742565 -148 CGGCATATATTGTTTTTGCTTGCTCATGG 3 10 0 TGTTTTTGCT 0.872381 -291 ATTGCACTTTTGTTTTAGCGATCGCATTTT 3 152 1 TGTTTTAGCG 0.842653 -149 CGCAAAAGGGTGCTTTTGCGTCTGCTCGGC 10 14 0 TGCTTTTGCG 0.97932 -68 CGCAAAAGCACCCTTTTGCGGCGCAAAAGT 10 24 1 CCCTTTTGCG 0.969429 -58 GCAAAAGCGCCACTTTTGCGCCGCAAAAGG 10 35 0 CACTTTTGCG 0.920822 -47 GCAAAAGTGGCGCTTTTGCGTCTGCTCGCG 10 46 1 CGCTTTTGCG 0.986186 -36 ********** Masking position 5 Map Score: 5.09977 Number of sites scoring better than the average of aligned sites = 660 Number in coding regions = 584 Number in noncoding regions = 76 Number of orfs with sites within 600 bp upstream = 91 Fraction of orfs with sites within 600 bp upstream = 0.0146161 Motif number 10 ACACTCCATCGGTCACCTGAGGCGGAGCTT 4 66 0 GGTCACCTGA 0.827789 -149 GGACTCAAGGGTGAGCCTGAGGTACACGCG 8 110 1 GTGAGCCTGA 0.927983 -42 CGATCCGTGGGTTTACCTGCGGATTTGTCG 9 28 1 GTTTACCTGC 0.884876 -247 AGTGAAATGGGTGGACCAGCGTTGACACGC 9 147 1 GTGGACCAGC 0.82252 -128 TAGCACACCAGTGAAGCTGCGCCGTTGACA 9 193 1 GTGAAGCTGC 0.873829 -82 TTACCAACGAGGTAGCCAGCATGTGACGGG 11 166 1 GGTAGCCAGC 0.925439 -135 ATCACACAACGGTAACCTGAAGGGAAAGAA 11 216 1 GGTAACCTGA 0.939838 -85 GATGCCACGGGTTAGCCTGCCTTAACA 15 8 0 GTTAGCCTGC 0.968288 -223 GCACAGGCGTGGGAACCTGCTCTACCACCC 15 84 1 GGGAACCTGC 0.983966 -147 ********** Masking position 7 Map Score: 4.79624 Number of sites scoring better than the average of aligned sites = 843 Number in coding regions = 795 Number in noncoding regions = 48 Number of orfs with sites within 600 bp upstream = 58 Fraction of orfs with sites within 600 bp upstream = 0.00931577 Motif number 11 CAAAAATGTGATACCAATCACAGAAT 1 7 0 ATACCAATCA 0.828915 -294 AATAGTTAAATCGCGAATCATAAAAAGCAA 2 45 1 TCGCGAATCA 0.808035 -240 TTAATTAAAAACACGAATCACATTGTTTTA 2 233 0 ACACGAATCA 0.940221 -52 ATGGTTCCGTATGCGACTCACAGGAGAAAT 3 280 1 ATGCGACTCA 0.915059 -21 TGTTGTGAAGACGCCATTCGCAGAGTTAGC 8 29 1 ACGCCATTCG 0.794897 -123 CGTGGCGGAAACACCAATCGCAGCACGCTT 8 59 0 ACACCAATCG 0.892003 -93 TTGAGTCCCGGTGCGAATCATGCCAACGCC 8 88 0 GTGCGAATCA 0.696242 -64 GGTGGTCGTGATACCACTCGATGGCAACGT 11 97 1 ATACCACTCG 0.748391 -204 GATCAAGTCCACGCCCGTCACATGCTGGCT 11 179 0 ACGCCCGTCA 0.760678 -122 CGGTCTCCGTACACGACTCATGCTGGACAC 13 13 1 ACACGACTCA 0.939086 -103 TTCGATGTTAACGCGAGTCAGACATGCGGT 13 76 1 ACGCGAGTCA 0.952674 -40 ********** Masking position 8 Map Score: 3.95258 Number of sites scoring better than the average of aligned sites = 556 Number in coding regions = 527 Number in noncoding regions = 29 Number of orfs with sites within 600 bp upstream = 38 Fraction of orfs with sites within 600 bp upstream = 0.00610344 Motif number 12 GTATAGCAACTGCGGAAAACATTAATGCAC 2 109 1 TGCGGAAAAC 0.94219 -176 ATAACTATATTGCCGCAAATTGCACTTTTG 3 134 1 TGCCGCAAAT 0.950664 -167 AAACTTGCTATGCAGAAATTTGCACAGTGC 5 16 1 TGCAGAAATT 0.926122 -180 TGAGCGGCTATGCAGAAAATTGCGCACTGT 5 39 0 TGCAGAAAAT 0.958999 -157 CGCAGGCCGTTGCCGAAATTCGCAAGTGAA 9 123 1 TGCCGAAATT 0.965806 -152 GGTCTTTCATTGCCGAAAACGCTGACGCCC 9 240 0 TGCCGAAAAC 0.977624 -35 ********** Masking position 7 Map Score: 3.05764 Number of sites scoring better than the average of aligned sites = 128 Number in coding regions = 116 Number in noncoding regions = 12 Number of orfs with sites within 600 bp upstream = 13 Fraction of orfs with sites within 600 bp upstream = 0.00208802 Motif number 13 TGTTTTATGGATCACCAATATTGAAAGTCA 1 121 0 ATCACCAATA 0.968652 -180 TGTTAAGTTCCTCACCAATATTGAAAACAC 1 178 0 CTCACCAATA 0.979834 -123 TAAAACTTTAATCACCAATATTGAAAATGT 1 237 0 ATCACCAATA 0.968652 -64 GTTAAGTAAAATCACAAATTTTGTATGTTT 2 187 0 ATCACAAATT 0.719266 -98 CAACCACTAACCCACCAATAGAGGGGTAGA 7 95 0 CCCACCAATA 0.95631 -157 AACCTCGCACCAATTAACAGTGCAA 7 237 0 CGCACCAATT 0.921453 -15 ********** Masking position 4 Map Score: 2.76469 Number of sites scoring better than the average of aligned sites = 74 Number in coding regions = 66 Number in noncoding regions = 8 Number of orfs with sites within 600 bp upstream = 7 Fraction of orfs with sites within 600 bp upstream = 0.00112432 Motif number 14 TGTATAGCAACTGCGGAAAACATTAATGCA 2 108 1 CTGCGGAAAA 0.867115 -177 GCCAACGCCGTGGCGGAAACACCAATCGCA 8 67 0 TGGCGGAAAC 0.911195 -85 GATCGACGTCCTGCGGATCCGG 9 3 0 CTGCGGATCC 0.982359 -272 TTGCGCCGCGGAGCCCGGCACCGCC 11 6 1 CCGCGGAGCC 0.964857 -295 AGGCACCAGGCGGAGGAACC 13 106 1 CGGAGGAACC 0.954938 -10 CGCCCACGACCGGCGGATACTCCCGGTGCC 15 52 0 CGGCGGATAC 0.976957 -179 CAGCGCTCGACTGCGGAACCTCGGTCACAC 15 179 0 CTGCGGAACC 0.990467 -52 ********** Masking position 7 Map Score: 5.75264 Number of sites scoring better than the average of aligned sites = 225 Number in coding regions = 217 Number in noncoding regions = 8 Number of orfs with sites within 600 bp upstream = 10 Fraction of orfs with sites within 600 bp upstream = 0.00160617 Motif number 15 AACTATGCGATCTGTATAGCAACTGCGGAA 2 96 1 TCTGTATAGC 0.963182 -189 TGTGCAAATTTCTGCATAGCAAGTTTTGGT 5 12 0 TCTGCATAGC 0.99053 -184 TGCGCAATTTTCTGCATAGCCGCTCATTCT 5 43 1 TCTGCATAGC 0.99053 -153 TCTGCCGAGCAGACGCAAAA 10 1 1 TCTGCCGAGC 0.973053 -81 ********** Masking position 3 Map Score: 2.08682 Number of sites scoring better than the average of aligned sites = 21 Number in coding regions = 18 Number in noncoding regions = 3 Number of orfs with sites within 600 bp upstream = 3 Fraction of orfs with sites within 600 bp upstream = 0.00048185