AlignACE version 2.2 July 7, 1998 /home/amcguire/bin/alignACE -i116_ecoli_mtub_100.orf -o116_ecoli_mtub_100.ace -a/home/amcguire/genomes/ORF_ecoli.txt -z/home/amcguire/genomes/ecoli.fna -g0.58 -x5 -e Parameter values: expect = 5 ncols = 10 npass = 1000 maxnpass = 100 nruns = 1000 maxnruns = 100 repeat = 15 maxreps = 3 nread = 500 ncycles = 1 fragment = 1 psfact = 0.1 gcback = 0.58 maxlen = 30 weight = 0.8 exclude = 1 Input sequences: #1 sapA 248 homolog of Salmonella peptide transport periplasmic protein Motif number 1 GTACGAATGTTGAACAGATGGCGACGTCAC 1 116 1 TGAACAGATG 0.989235 -133 GGTTGGTCGTTGAACAGGTGACGTCGCCAT 1 133 0 TGAACAGGTG 0.99002 -116 GTTCAGGGGTCGTAATGAGGGCCGTGTGGT 1 160 0 CGTAATGAGG 0.94735 -89 TTACCGTTTGTGTTCAGGGGTCGTAATGAG 1 171 0 TGTTCAGGGG 0.989831 -78 AGGGATTTTATGTAAAGAGGCTATCTTACT 1 222 0 TGTAAAGAGG 0.992462 -27 ********** Masking position 7 Map Score: 5.21437 Number of sites scoring better than the average of aligned sites = 235 Number in coding regions = 206 Number in noncoding regions = 29 Number of orfs with sites within 600 bp upstream = 32 Fraction of orfs with sites within 600 bp upstream = 0.00513974 Motif number 2 AAGCACGCTGCAGTGCGTGTCATTGTTA 1 9 0 CAGTGCGTGT 0.980833 -240 ACGCACTGCAGCGTGCTTTAATGGGTTTCT 1 21 1 GCGTGCTTTA 0.92189 -228 TAGCGAGGAGCAGAGCGGTACGATCGTGAG 1 49 0 CAGAGCGGTA 0.913974 -200 CTAAGTTGTTCTGTGAGTTATAGTTATAGA 1 76 1 CTGTGAGTTA 0.950354 -173 ACATTCGTACCCGTGATTTCTCTATAACTA 1 96 0 CCGTGATTTC 0.941124 -153 GTAATGAGGGCCGTGTGGTTGGTCGTTGAA 1 149 0 CCGTGTGGTT 0.985342 -100 TTTGCCATTACCGTTTGTGTTCAGGGGTCG 1 178 0 CCGTTTGTGT 0.897112 -71 ********** Masking position 3 Map Score: 1.8139 Number of sites scoring better than the average of aligned sites = 2782 Number in coding regions = 2622 Number in noncoding regions = 160 Number of orfs with sites within 600 bp upstream = 192 Fraction of orfs with sites within 600 bp upstream = 0.0308384 Motif number 3 GGGTTTCTCACGATCGTACCGCTCTGCTCC 1 43 1 CGATCGTACC 0.996195 -206 ATCTGTTCAACATTCGTACCCGTGATTTCT 1 105 0 CATTCGTACC 0.991664 -144 TGTAAAGAGGCTATCTTACTAATATTTAAT 1 212 0 CTATCTTACT 0.967761 -37 ********** Masking position 8 Map Score: 0.369612 Number of sites scoring better than the average of aligned sites = 77 Number in coding regions = 73 Number in noncoding regions = 4 Number of orfs with sites within 600 bp upstream = 3 Fraction of orfs with sites within 600 bp upstream = 0.00048185 Motif number 4 ********** No masking Map Score: -4.23894e-13 Number of sites scoring better than the average of aligned sites = 0 Number in coding regions = 0 Number in noncoding regions = 0 Number of orfs with sites within 600 bp upstream = 0 Fraction of orfs with sites within 600 bp upstream = 0 Motif number 5 ********** No masking Map Score: -4.23894e-13 Number of sites scoring better than the average of aligned sites = 0 Number in coding regions = 0 Number in noncoding regions = 0 Number of orfs with sites within 600 bp upstream = 0 Fraction of orfs with sites within 600 bp upstream = 0 Motif number 6 ********** No masking Map Score: -4.23894e-13 Number of sites scoring better than the average of aligned sites = 0 Number in coding regions = 0 Number in noncoding regions = 0 Number of orfs with sites within 600 bp upstream = 0 Fraction of orfs with sites within 600 bp upstream = 0