AlignACE version 2.2 July 7, 1998 alignACE -z/skink1/amcguire/genomes/ecoli.fna -a/home/amcguire/alignace/lib/ORF_ecoli.txt -b/home/amcguire/alignace/lib/bimes_ecoli.txt -k0 -l300 -jfnr.reg -g0.5 -x5 Parameter values: expect = 5 ncols = 10 npass = 1000 maxnpass = 100 nruns = 1000 maxnruns = 100 repeat = 15 maxreps = 3 nread = 500 ncycles = 1 fragment = 1 psfact = 0.1 gcback = 0.5 maxlen = 30 weight = 0.8 exclude = 157 Input sequences: #1 cydA 300 bp cydA 523 1-1572 CDS #2 focA 300 bp focA 285 1-858 CDS #3 ndh 271 bp ndh 434 1-1305 CDS #4 narK 300 bp narK 463 1-1392 CDS #5 narG 300 bp narG 1247 1-3744 CDS #6 fdnG 231 bp fdnG 1015 1-3048 CDS #7 ansB 175 bp ansB 348 1-1047 CDS #8 nirB 261 bp nirB 847 1-2544 CDS #9 nrfA 300 bp nrfA 478 1-1437 CDS Motif number 1 TTCAGCCAATTTGATCGAGAACAATTTATCT 1 8 0 TTGAGAACAA 0.813491 -293 TTTGCATTTTTCGACGGTGATCAATTTATAGCTA 1 53 0 TCGAGTACAA 0.884916 -248 AGATGAGAAATTGATATAGATCATATCTCGCCTG 2 227 0 TTGATAACAT 0.63114 -74 CATACCAAAATTGATGTTAATCAAGTTTTGTTGC 3 122 0 TTGATTACAA 0.618885 -150 TTATCTATCATTGATATTTATCATTACCCATAGT 4 190 0 TTGATTACAT 0.916195 -111 ATCTTATCGTTTGATTTACATCAAATTGCCTTTA 4 228 1 TTGATAACAA 0.499753 -73 CCCATCACTCTTGATCGTTATCAATTCCCACGCT 5 196 1 TTGAGTACAA 0.919688 -105 GAGCGTTACCTTGCCCTTAAACATTAGCAATGTC 5 236 1 TTGCTTACAT 0.842698 -65 CCTTCGCAAATTGACCTACCTCAATAGCGGTAGA 6 99 0 TTGATACCAA 0.588912 -133 CTGAATCTTTTTGCGCGAAATCAAAAAGAACATC 6 154 0 TTGCGAACAA 0.750179 -78 TATGGGAAATTTGACGTTAAACAATTTACAACGT 7 105 0 TTGATTACAA 0.979187 -71 AAAGGTGAATTTGATTTACATCAATAAGCGGGGT 8 190 1 TTGATAACAA 0.499753 -72 AGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGA 9 44 1 TTGAGTACAA 0.980868 -257 ACTGTGAATGTTGTCTTTAATCAATTGTAAGTGC 9 160 0 TTGTTTACAA 0.842695 -141 **** ** * *** Masking position 13 Map Score: 21.6283 Number of sites scoring better than the average of aligned sites = 59 Number in coding regions = 31 Number in noncoding regions = 28 Number of orfs with sites within 600 bp upstream = 32 Fraction of orfs with sites within 600 bp upstream = 0.00513974 Motif number 2 GGGATCAATTTGAGTTTTTATTAACATGTTTGCAA 1 142 1 TGTTATTAAC 0.846653 -159 TAAATACAAATTACCGATATTTAACCTTATAATTA 2 12 0 TCATTTTAAC 0.845444 -289 CGCCTGATGCTCCCTTTTAATTAACTGTTTTAGCG 2 76 1 TCTTATTAAC 0.983922 -225 ACTAACTCTCTCTTTATTAAGTCGGCGACGA 2 280 0 TCTTATTAAG 0.916627 -21 TAAAGTCGCCTATCTTTTCAGCAACAAAACTTGAT 3 102 1 TCTTAGCAAC 0.874153 -170 GTTATTAACATAGCTCTTAATTAACAACAGGTTAC 3 207 0 TCTTATTAAC 0.983922 -65 GCTATGTTAATAACCATTAATTAACAATTGGTTAA 3 228 1 TCTTATTAAC 0.983922 -44 AAAGTTATCTTATCGTTTGATTTACATCAAATTGC 4 222 1 TCTTATTTAC 0.848536 -79 TGTTATGTGGTGGCTGTTAATTATCCTAAAGGGGT 5 133 1 TCTTATTATC 0.848536 -168 TAAATCGACATTGCTAATGTTTAAGGGCAAGGTAA 5 241 0 TCATTTTAAG 0.495647 -60 CTGAATTTTATAGCATTTTTTTAACTGGCCTGTCA 6 48 1 TCTTTTTAAC 0.969248 -184 ATATTCACGTTGTAAATTGTTTAACGTCAAATTTC 7 99 1 TATTTTTAAC 0.657369 -77 CCGCCTACCTTAACGATTCAGCAACCCCGCTTATT 8 212 0 TCTTAGCAAC 0.874153 -50 AGTCAGTATCTTCCTCTTTTTCAACAGCATGCATA 9 102 0 TCTTTTCAAC 0.930198 -199 TTACATGCACTTACAATTGATTAAAGACAACATTC 9 154 1 TCTTATTAAA 0.801223 -147 * * ** ****** Masking position 8 Map Score: 13.6498 Number of sites scoring better than the average of aligned sites = 248 Number in coding regions = 184 Number in noncoding regions = 64 Number of orfs with sites within 600 bp upstream = 77 Fraction of orfs with sites within 600 bp upstream = 0.0123675 Motif number 3 ATATATTACGCCGCAAAATCCTTACAATAAACAG 1 104 0 CGCAATCCTA 0.92912 -197 TGAATTTTTTCCGCATCCTCCGCTAAAACAGTTA 2 97 0 CGCATTCCCT 0.993425 -204 CGAACGCCCTGCATACACCCCTCACTCTATAT 3 9 1 CGCATACCCT 0.945943 -263 TGATCTCACCCGGTAAAGTCGCCTATCTTTTCAG 3 89 1 CGTAATCGCT 0.84452 -183 TCTGGAATGGCAGTATTCTCGGCTATTGGCTGAA 4 32 1 CGTATTCGCT 0.912837 -269 TTTCACGGTGCTGTAAAATCCCTACCCTTACCGA 4 124 0 CGTAATCCTA 0.808721 -177 TATTTTAAGACGGCATAATACTTTTTTATGCCGT 7 16 1 CGCATTACTT 0.871309 -160 CGTGAACGCTACGCATTATCCCTTAGCTCTGTAT 7 136 0 AGCATTCCTT 0.777602 -40 TATCGTCGTCCGGCCTTTTCCTCTCTTCCCCCGC 8 31 1 CGCCTTCCCT 0.962875 -231 TTATACAAATCAGCAATATACCCATTAAGGAGTA 8 153 1 CGCAATACCA 0.849548 -109 GCATGCATAACTGCATGTTCCTCAAAGAATTAAT 9 77 0 CGCATTCCCA 0.987399 -224 TAGAGTTAGTCAGTATCTTCCTCTTTTTCAACAG 9 110 0 CGTATTCCCT 0.979891 -191 * **** *** ** Masking position 3 Map Score: 10.5524 Number of sites scoring better than the average of aligned sites = 647 Number in coding regions = 599 Number in noncoding regions = 48 Number of orfs with sites within 600 bp upstream = 56 Fraction of orfs with sites within 600 bp upstream = 0.00899454 Motif number 4 GGTTAAATATCGGTAATTTGTATTTAATAAATA 2 21 1 CGATTTGTAT 0.635856 -280 TTGATTAACATCAATTTTGGTATGACCAATGCA 3 132 1 TCTTTGGTAT 0.641627 -140 TAACTCTTTCCGGGTATGGGTATACTTCAGCCA 4 58 0 CGATGGGTAT 0.968267 -243 GTCACTGTACTCACTATGGGTAATGATAAATAT 4 178 1 TCATGGGTAA 0.964408 -123 TAAGGAGTATTCCCCATGGGTAACATATTAATG 5 32 0 TCATGGGTAA 0.964408 -269 AAGATTTTTATGCAGATGGGTATTAAGGAGTAT 5 55 0 TGATGGGTAT 0.967684 -246 TGTTAATTATCCTAAAGGGGTATCTTAGGAATT 5 147 1 CCAGGGGTAT 0.967855 -154 CTTTCCTCTTTTTTCAGGGGTATTACTGCGCGA 6 207 0 TTAGGGGTAT 0.743371 -25 ATCCCTTAGCTCTGTATGGGAAATTTGACGTTA 7 120 0 TCATGGGAAA 0.742501 -56 ATGGGTATATTGCTGATTTGTATAAATTTTCTT 8 145 0 TGATTTGTAT 0.631487 -117 TTTATATACTCCTTAATGGGTATATTGCTGATT 8 160 0 CCATGGGTAT 0.98351 -102 ACTGACTAACTCTAAAGTGGTATTTTACATGCA 9 130 1 TCAGTGGTAT 0.894662 -171 ACATTGCTCGCCCCTATGTGTAACAAATAACCA 9 195 0 CCATGTGTAA 0.846084 -106 ** ******** Masking position 12 Map Score: 7.65628 Number of sites scoring better than the average of aligned sites = 505 Number in coding regions = 462 Number in noncoding regions = 43 Number of orfs with sites within 600 bp upstream = 46 Fraction of orfs with sites within 600 bp upstream = 0.00738837 Motif number 5 TTCAGCCAATTTGATCGAGAACAATTTATCT 1 10 0 TTGATGAAAC 0.770501 -291 TTTGCATTTTTCGACGGTGATCAATTTATAGC 1 55 0 TCGACGTATC 0.795923 -246 AGATGAGAAATTGATATAGATCATATCTCGCC 2 229 0 TTGATTAATC 0.953949 -72 CATACCAAAATTGATGTTAATCAAGTTTTGTT 3 124 0 TTGATTTATC 0.959997 -148 GTGTGGTTAGTCGCTTTACATCGGTAAGGGTA 4 104 1 TCGCTTAATC 0.694015 -197 TTATCTATCATTGATATTTATCATTACCCATA 4 192 0 TTGATTTATC 0.959997 -109 TAAAGGCAATTTGATGTAAATCAAACGATAAG 4 230 0 TTGATTAATC 0.953949 -71 CCCATCACTCTTGATCGTTATCAATTCCCACG 5 196 1 TTGATGTATC 0.92375 -105 GAGCGTTACCTTGCCCTTAAACATTAGCAATG 5 236 1 TTGCCTTAAC 0.731247 -65 CCTTCGCAAATTGACCTACCTCAATAGCGGTA 6 101 0 TTGACTACTC 0.769018 -131 TATGGGAAATTTGACGTTAAACAATTTACAAC 7 107 0 TTGACTTAAC 0.887017 -69 ACCCCGCTTATTGATGTAAATCAAATTCACCT 8 192 0 TTGATTAATC 0.953949 -70 ACTGTGAATGTTGTCTTTAATCAATTGTAAGT 9 162 0 TTGTCTTATC 0.756522 -139 ***** ** *** Masking position 1 Map Score: 10.6139 Number of sites scoring better than the average of aligned sites = 208 Number in coding regions = 157 Number in noncoding regions = 51 Number of orfs with sites within 600 bp upstream = 58 Fraction of orfs with sites within 600 bp upstream = 0.00931577 Motif number 6 AACAGGTTTTTGTTGAAGCAAATTTGCATTTT 1 77 0 TGTTGGCAAA 0.936818 -224 ATTGTAAGGATTTTGCGGCGTAATATATACGT 1 110 1 TTTTGGCGTA 0.847703 -191 TTTAATTAACTGTTTTAGCGGAGGATGCGGAA 2 91 1 TGTTTGCGGA 0.939066 -210 ACTCGCATGGTTTTTATGCAAATACGGAGTAA 2 180 0 TTTTTGCAAA 0.939454 -121 TATTGTTTATTATTTGAGCGAATTTGTGAGAG 3 45 0 TATTTGCGAA 0.781673 -227 GTTAATCAAGTTTTGTTGCTGAAAAGATAGGC 3 109 0 TTTTGGCTGA 0.907536 -163 ACTATTAAGATTTTTATGCAGATGGGTATTAA 5 62 0 TTTTTGCAGA 0.943859 -239 AGGCGGATTATTTTGTGGCAAACAGATGTTCT 6 130 1 TTTTGGCAAA 0.954175 -102 ATTCTGAATCTTTTTGCGCGAAATCAAAAAGA 6 159 0 TTTTTGCGAA 0.952332 -73 TGATATTTCATGTTTGTGCAAAAAATAGACAA 8 97 0 TGTTTGCAAA 0.917004 -165 ATTAATCAACTTTTGTTGCTGACCTTCAAAAA 9 51 0 TTTTGGCTGA 0.907536 -250 ***** ***** Masking position 12 Map Score: 10.5968 Number of sites scoring better than the average of aligned sites = 372 Number in coding regions = 307 Number in noncoding regions = 65 Number of orfs with sites within 600 bp upstream = 85 Fraction of orfs with sites within 600 bp upstream = 0.0136524 Motif number 7 ACAAAACTTGATTAACATCAATTTTGGTAT 3 125 1 ATTAACATCA 0.759816 -147 ATAGCTCTTAATTAACAACAGGTTACAGCA 3 203 0 ATTAACAACA 0.759816 -69 AGGGTAGGGATTTTACAGCACCGTGAAAAA 4 130 1 TTTTACAGCA 0.793132 -171 CTTTAGGATAATTAACAGCCACCACATAAC 5 134 0 ATTAACAGCC 0.751579 -167 ATTGCTAATGTTTAAGGGCAAGGTAACGCT 5 237 0 TTTAAGGGCA 0.858198 -64 CAGCTTAATTTTTAACGGCAAGAGAGACAA 6 21 0 TTTAACGGCA 0.981069 -211 AAACGAAGAATTAAACGGCATAAAAAAGTA 7 34 0 TTAAACGGCA 0.93509 -142 TTGTAAATTGTTTAACGTCAAATTTCCCAT 7 108 1 TTTAACGTCA 0.910753 -68 AATTGTGCAAATAAACGGCAGGGTAATTTT 9 25 1 ATAAACGGCA 0.894669 -276 CTTCCTCTTTTTCAACAGCATGCATAACTG 9 98 0 TTCAACAGCA 0.864246 -203 TTTTATTGTTTTTCACGACAGTAACCGCAC 9 241 0 TTTCACGACA 0.680328 -60 ********** Masking position 5 Map Score: 5.32169 Number of sites scoring better than the average of aligned sites = 838 Number in coding regions = 768 Number in noncoding regions = 70 Number of orfs with sites within 600 bp upstream = 78 Fraction of orfs with sites within 600 bp upstream = 0.0125281 Motif number 8 CTAAATTACCGCCTTTCAGCCAATTTGATCG 1 25 0 GCCTTCAGCC 0.915398 -276 CGAGTTACGGGCCTATAAGCCAGGCGAGATA 2 207 1 GCCTTAAGCC 0.965876 -94 TCCTGTGGGAGCCTGTCGGCCCTCTGATAAA 5 272 0 GCCTTCGGCC 0.968379 -29 TTTTTAACTGGCCTGTCAGGCAGTGGTGCGT 6 65 1 GCCTTCAGGC 0.525041 -167 **** ****** Masking position 6 Map Score: 2.5992 Number of sites scoring better than the average of aligned sites = 29 Number in coding regions = 24 Number in noncoding regions = 5 Number of orfs with sites within 600 bp upstream = 4 Fraction of orfs with sites within 600 bp upstream = 0.000642467 Motif number 9 ACAATAAACTCTGTTTTTTGATCTCACCCGGTAAAGTCG 3 71 1 CTTTTTCCCC 0.87785 -201 TAGCGAAGAACATTTTCATTGCTGTAACCTGTTGTTAAT 3 184 1 CTTTTCTTCC 0.98866 -88 CTCTGATACTCGTTTCGATGTCTGCCACCTTAGTGTCTG 4 266 0 CTTCTCTCCC 0.982271 -35 GCTCGCTGTTTTGTCTCTCTTGCCGTTAAAAATT 6 6 1 CTTTTCTTCC 0.98866 -226 TTTAATTCTTCGTTTTGTTACCTGCCTCTAACTTTGTAG 7 49 1 CTTTTCTCCT 0.958943 -127 CGTCGTCCGGCCTTTTCCTCTCTTCCCCCGCTACGTGCA 8 34 1 CTTTTCTCCC 0.994477 -228 TTTTGCCTCGATTTCTTTTCTATTACCGCCTACCTTA 8 235 0 CTTTTCTTCC 0.98866 -27 * *** * ** * ** Masking position 9 Map Score: 2.63459 Number of sites scoring better than the average of aligned sites = 104 Number in coding regions = 93 Number in noncoding regions = 11 Number of orfs with sites within 600 bp upstream = 15 Fraction of orfs with sites within 600 bp upstream = 0.00240925 Motif number 10 TTTATGCAAATACGGAGTAAATATTTGATT 2 170 0 TACGGAGTAA 0.87317 -131 ACACGGCAAATAAGGAGTAACTCTTTCCGG 4 78 0 TAAGGAGTAA 0.962853 -223 GCAGACATCGAAACGAGTATCAGAGGTGTC 4 280 1 AAACGAGTAT 0.71586 -21 AGATGGGTATTAAGGAGTATTCCCCATGGG 5 45 0 TAAGGAGTAT 0.976516 -256 TAATTATCCTAAAGGGGTATCTTAGGAATT 5 150 1 AAAGGGGTAT 0.937189 -151 TCCTCTTTTTTCAGGGGTATTACTGCGCGA 6 207 0 TCAGGGGTAT 0.915437 -25 CATACAGAGCTAAGGGATAATGCGTAGCGT 7 135 1 TAAGGGATAA 0.739387 -41 ATATACCCATTAAGGAGTATATAAAGGTGA 8 168 1 TAAGGAGTAT 0.976516 -94 ********** Masking position 9 Map Score: 4.64327 Number of sites scoring better than the average of aligned sites = 95 Number in coding regions = 64 Number in noncoding regions = 31 Number of orfs with sites within 600 bp upstream = 37 Fraction of orfs with sites within 600 bp upstream = 0.00594282 Motif number 11 TTACGCCGTTTTTGTGTGCATTCACATGGT 1 184 1 TTTGTGTGCA 0.924028 -117 ACTCGCATGGTTTTTATGCAAATACGGAGT 2 182 0 TTTTTATGCA 0.975673 -119 AATCTCATAATTTTTATGAAGTCACTGTAC 4 158 1 TTTTTATGAA 0.861226 -143 ACTATTAAGATTTTTATGCAGATGGGTATT 5 64 0 TTTTTATGCA 0.975673 -237 GCATAATACTTTTTTATGCCGTTTAATTCT 7 28 1 TTTTTATGCC 0.936187 -148 TGATATTTCATGTTTGTGCAAAAAATAGAC 8 99 0 TGTTTGTGCA 0.924025 -163 ********** Masking position 5 Map Score: 2.33362 Number of sites scoring better than the average of aligned sites = 51 Number in coding regions = 25 Number in noncoding regions = 26 Number of orfs with sites within 600 bp upstream = 32 Fraction of orfs with sites within 600 bp upstream = 0.00513974 Motif number 12 TCACACGCATGCCCCAATAGAAATTTGTTTG 1 228 0 GCCCCATAGA 0.954728 -73 CCGAGAGTGAACCCCGTTAGAAAGGGTCACA 1 254 0 ACCCCTTAGA 0.990395 -47 CGTGACCCCCTTAAATTTATTAACC 3 257 0 ACCCCTTAAA 0.941007 -15 TTCGATGTCTGCCACCTTAGTGTCTGTAGCT 4 261 0 GCCACTTAGT 0.897737 -40 TTCCTAAGATACCCCTTTAGGATAATTAACA 5 147 0 ACCCCTTAGG 0.978925 -154 CATGTAAAATACCACTTTAGAGTTAGTCAGT 9 130 0 ACCACTTAGA 0.972097 -171 ***** ***** Masking position 8 Map Score: 2.0393 Number of sites scoring better than the average of aligned sites = 15 Number in coding regions = 8 Number in noncoding regions = 7 Number of orfs with sites within 600 bp upstream = 10 Fraction of orfs with sites within 600 bp upstream = 0.00160617 Motif number 13 ATTTGATCGAGAACAATTTATCT 1 4 0 GAACAATTTA 0.60869 -297 CGCAAAATCCTTACAATAAACAGGTTTTTG 1 97 0 TTACAATAAA 0.414774 -204 TCCTCCGCTAAAACAGTTAATTAAAAGGGA 2 86 0 AAACAGTTAA 0.744707 -215 AAATAAATTTTAAAAATTAACAAATGAGTT 2 130 0 TAAAAATTAA 0.77812 -171 ATTATCCAAATAAAAATAAATTTTAAAAAT 2 143 0 TAAAAATAAA 0.682263 -158 CTCAAATAATAAACAATAAACTCTGTTTTT 3 59 1 AAACAATAAA 0.8773 -213 GCTATGTTAATAACCATTAATTAACAATTG 3 228 1 TAACCATTAA 0.64741 -44 GTAGTTATTTAAACTATTAAGATTTTTATG 5 76 0 AAACTATTAA 0.516782 -225 TGATGGGGATGAAAAATAAAGTAAATTCCT 5 173 0 GAAAAATAAA 0.604565 -128 CTCTTGCCGTTAAAAATTAAGCTGAATTTT 6 27 1 TAAAAATTAA 0.77812 -205 GCGAGGAAACTAACAGTAAAATTCTGAATC 6 181 0 TAACAGTAAA 0.674082 -51 GTAACAAAACGAAGAATTAAACGGCATAAA 7 40 0 GAAGAATTAA 0.67437 -136 ATTTGACGTTAAACAATTTACAACGTGAAT 7 101 0 AAACAATTTA 0.653481 -75 CATGTTCCTCAAAGAATTAATCAACTTTTG 9 68 0 AAAGAATTAA 0.715161 -233 CCCCTATGTGTAACAAATAACCACACTGTG 9 188 0 TAACAAATAA 0.536613 -113 CTGTCGTGAAAAACAATAAAAACCGCCATT 9 250 1 AAACAATAAA 0.8773 -51 ********** Masking position 3 Map Score: 3.57107 Number of sites scoring better than the average of aligned sites = 332 Number in coding regions = 205 Number in noncoding regions = 127 Number of orfs with sites within 600 bp upstream = 161 Fraction of orfs with sites within 600 bp upstream = 0.0258593 Motif number 14 TTAGAAAGGGTCACACGCATGCCCCAATAGA 1 238 0 TACACGCATG 0.945291 -63 TATTGTTACTTTATTCGCCTGATGCTCCCTT 2 61 1 TATTCGCCTG 0.97557 -240 TATAGGCCCGTAACTCGCATGGTTTTTATGC 2 193 0 TACTCGCATG 0.957444 -108 CTCGCCTGGCTTATAGGCCCGTAACTCGCAT 2 204 0 TATAGGCCCG 0.79927 -97 ATATAGATCATATCTCGCCTGGCTTATAGGC 2 217 0 TTCTCGCCTG 0.94482 -84 AGCATTTTTTTAACTGGCCTGTCAGGCAGTG 6 59 1 TACTGGCCTG 0.972068 -173 CCTCGCGCAGTAATACCCCTGAAAAAAGAGG 6 205 1 TATACCCCTG 0.854982 -27 * ********* Masking position 1 Map Score: 1.13853 Number of sites scoring better than the average of aligned sites = 544 Number in coding regions = 501 Number in noncoding regions = 43 Number of orfs with sites within 600 bp upstream = 40 Fraction of orfs with sites within 600 bp upstream = 0.00642467 Motif number 15 ATGAGAAATTGATATAGATCATATCTCGCC 2 229 0 GATATAGATC 0.639813 -72 AACAAAACTTGATTAACATCAATTTTGGTA 3 124 1 GATTAACATC 0.954043 -148 GTGGTTAGTCGCTTTACATCGGTAAGGGTA 4 106 1 GCTTTACATC 0.937032 -195 TATGGGTAATGATAAATATCAATGATAGAT 4 192 1 GATAAATATC 0.609943 -109 CTTATCGTTTGATTTACATCAAATTGCCTT 4 230 1 GATTTACATC 0.866055 -71 AGGTGAATTTGATTTACATCAATAAGCGGG 8 192 1 GATTTACATC 0.866055 -70 ********** Masking position 6 Map Score: 2.70688 Number of sites scoring better than the average of aligned sites = 53 Number in coding regions = 40 Number in noncoding regions = 13 Number of orfs with sites within 600 bp upstream = 17 Fraction of orfs with sites within 600 bp upstream = 0.00273049