AlignACE version 2.2 July 7, 1998 alignACE -a/home/amcguire/genomes/ORF_ecoli.txt -z/home/amcguire/genomes/ecoli.fna -ilacI_ecoli_bsub_300.orf -g0.5 -x5 Parameter values: expect = 5 ncols = 10 npass = 1000 maxnpass = 100 nruns = 1000 maxnruns = 100 repeat = 15 maxreps = 3 nread = 500 ncycles = 1 fragment = 1 psfact = 0.1 gcback = 0.5 maxlen = 30 weight = 0.8 exclude = 0 Input sequences: #1 lacA 65 thiogalactoside acetyltransferase #2 lacY 51 galactoside permease (M protein) #3 lacZ 122 beta-D-galactosidase #4 lacI 76 transcriptional repressor of the lac operon #5 mhpR 76 transcriptional regulator for mhp operon Motif number 1 GTCGGATGCGGCGCGAGCGCCTTATCCGACCAA 1 20 1 GCGCGAGCTT 0.990187 -46 CCCGTATTTCGCGTAAGGAAATCCATT 2 35 1 GCGTAAGATC 0.971391 -17 GCGCAACGCAATTAATGTGAGTT 3 1 1 GCGCAACATT 0.997427 -122 GGAATTGTGAGCGGATAACAATTTCACACAGGA 3 93 1 GCGGATAATT 0.928616 -30 CCATCGAATGGCGCAAAACCTTTCGCGGTATGG 4 13 1 GCGCAAACTT 0.994104 -64 ATGATAGCGCCCGGAAGAGAGTCAATTCAGGGT 4 47 1 CCGGAAGATC 0.938758 -30 TGTACTGTGCGCGCAACGACATTTTGTCCGAGT 5 25 0 GCGCAACCTT 0.997375 -52 GCACAGTACAGCGCAACTTATTTTGTTAAAAAC 5 48 1 GCGCAACATT 0.997426 -29 ******* * ** Masking position 12 Map Score: 12.1452 Number of sites scoring better than the average of aligned sites = 627 Number in coding regions = 564 Number in noncoding regions = 63 Number of orfs with sites within 600 bp upstream = 76 Fraction of orfs with sites within 600 bp upstream = 0.0122069 Motif number 2 CAATGTCGGATGCGGCGCGAGCGCCTTATC 1 16 1 TGCGGCGCGA 0.966205 -50 AATGGATTTCCTTACGCGAAATACGGG 2 35 0 TTCCTTACGC 0.977729 -17 TCACATTAATTGCGTTGCGC 3 1 0 TGCGTTGCGC 0.994751 -122 AATGGCGCAAAACCTTTCGCGGTATGGCAT 4 19 1 AACCTTTCGC 0.892757 -58 ATTGACTCTCTTCCGGGCGCTATCATGCCA 4 43 0 TTCCGGGCGC 0.982958 -34 TTCAGTACCTCACGACTCGGACAAA 5 6 1 TACCTCACGA 0.92651 -71 AGTTGCGCTGTACTGTGCGCGCAACGACAT 5 36 0 TACTGTGCGC 0.982578 -41 TTTAACAAAATAAGTTGCGCTGTACTGTGC 5 48 0 TAAGTTGCGC 0.962305 -29 ********** Masking position 8 Map Score: 7.29862 Number of sites scoring better than the average of aligned sites = 3007 Number in coding regions = 2815 Number in noncoding regions = 192 Number of orfs with sites within 600 bp upstream = 191 Fraction of orfs with sites within 600 bp upstream = 0.0306778 Motif number 3 CGCGCCGCATCCGACATTGATTGC 1 2 0 CCGCATTGTG 0.968672 -64 TGCGATCACTCCGTTATGATATGTTGGTCGGAT 1 43 0 CCGTATGATG 0.978265 -23 GAAATACGGGCAGACATGGCCTGCCCGGTTATT 2 12 0 CAGCATGGTG 0.672334 -40 CATAAAGTGTAAAGCCTGGGGTGCCTAATGAGT 3 39 0 AAACCTGGTG 0.869012 -84 ATACGAGCCGGAAGCATAAAGTGTAAAGCCTGG 3 53 0 GAACATAATG 0.78089 -70 GAAAGGTTTTGCGCCATTCGATGGTG 4 4 0 GCGCATTCTG 0.960202 -73 AAAACCTTTCGCGGTATGGCATGATAGCGCCCG 4 27 1 GCGTATGGTG 0.986141 -50 ATTCACCACCCTGAATTGACTCTCTTCC 4 59 0 CCACCTGATG 0.966929 -18 *** ***** ** Masking position 7 Map Score: 4.43397 Number of sites scoring better than the average of aligned sites = 1577 Number in coding regions = 1460 Number in noncoding regions = 117 Number of orfs with sites within 600 bp upstream = 95 Fraction of orfs with sites within 600 bp upstream = 0.0152586 Motif number 4 TCAATGTCGGATGCGGCGCGAGCGCCTTAT 1 15 1 ATGCGGCGCG 0.96208 -51 CGAGCGCCTTATCCGACCAACATATCATAA 1 33 1 ATCCGACCAA 0.937434 -33 TAATAACCGGGCAGGCCATGTCTG 2 5 1 AACCGGGCAG 0.986269 -47 CTTACGCGAAATACGGGCAGACATGGCCTG 2 22 0 ATACGGGCAG 0.992246 -30 TCCACACAACATACGAGCCGGAAGCATAAA 3 66 0 ATACGAGCCG 0.992322 -57 ACGACATTTTGTCCGAGTCGTGAGGTACTG 5 13 0 GTCCGAGTCG 0.953299 -64 ********** Masking position 5 Map Score: 3.4435 Number of sites scoring better than the average of aligned sites = 1147 Number in coding regions = 1054 Number in noncoding regions = 93 Number of orfs with sites within 600 bp upstream = 78 Fraction of orfs with sites within 600 bp upstream = 0.0125281 Motif number 5 ********** No masking Map Score: -1.88842e-14 Number of sites scoring better than the average of aligned sites = 0 Number in coding regions = 0 Number in noncoding regions = 0 Number of orfs with sites within 600 bp upstream = 0 Fraction of orfs with sites within 600 bp upstream = 0 Motif number 6 ********** No masking Map Score: -1.88842e-14 Number of sites scoring better than the average of aligned sites = 0 Number in coding regions = 0 Number in noncoding regions = 0 Number of orfs with sites within 600 bp upstream = 0 Fraction of orfs with sites within 600 bp upstream = 0 Motif number 7 ********** No masking Map Score: -1.88842e-14 Number of sites scoring better than the average of aligned sites = 0 Number in coding regions = 0 Number in noncoding regions = 0 Number of orfs with sites within 600 bp upstream = 0 Fraction of orfs with sites within 600 bp upstream = 0