Prashant Mali1,3, John Aach1,3, Jehyuk Lee1,2, Daniel Levner1,2, Lisa Nip2, George M. Church1,2
1
Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA.
2 Wyss Institute for Biologically Inspired
Engineering, Harvard University, Cambridge, Massachusetts, USA.
3 These
authors contributed equally to this work.
ABSTRACT: We report an
approach to barcode cells through cell-surface expression of programmable
zinc-finger DNA-binding domains (surface zinc fingers, sZFs).
We show that sZFs enable sequence-specific labeling
of living cells by dsDNA, and we develop a sequential
labeling approach to image more than three cell types in mixed populations
using three fluorophores. We demonstrate the
versatility of sZFs through applications in which
they serve as surrogate reporters, function as selective cell capture reagents
and facilitate targeted cellular delivery of viruses.
This web site provides access to supplemental data files, images, and software related to the image analysis described in the article.
· For data files and images processed or generated by our image analysis routines, right click and download the zip file at this link.
·
To obtain the image analysis software, you can
download a zip file only after going here to
accept license terms.
Documentation for the files and images, and for the software, is included with the respective zip files.
Please contact John Aach with any questions or suggestions related to this material.
Last modified: 3/24/2013 4:38 PM