This lead to a whole genome shotgun approach using a set of vectors
designed for bidirectional end sequencing:
Church, G.M., and Kieffer-Higgins, S. (1988) Multiplex
DNA sequencing Science 240: 185-188.
This concept was applied to E.coli genomic cosmid shotgun sequencing:
Richterich,P., Lakey,N., Gryan,G., Jaehn,L., Mintz,L., Robison,K.
and Church,G.M. (1993-4) Automated multiplex sequencing of E.coli genomic
and plasmid DNA, Genbank accession numbers: U00007,
U00008,
U00009
and applied to a whole genome shotgun sequence:
Smith, D.R., et al. (1997) The
Complete Genome Sequence of Methanobacterium thermoautotrophicum Strain
delta-H: Functional Analysis and Comparative Genomics. J. Bacteriol.
Nov. 179: 7135-7155
A more automated, rapid and robust assembly algorithm using distance constraints between the ends of clones is being developed by Tim Chen.
Most of this work was supported by the Department of Energy grant DEFG02-87ER60565 to George Church.
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