Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.100009
Description:acyl-Coenzyme A oxidase 1, palmitoyl
Best-Of-UniGene (BOU) SequenceBC008767
Genomic Coordiantes Displayed:Bases 386497 to 426796 of contig Hs17_10798_24
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingHs.100009.jpeg
Best sense EST/protein match:BC008767 matched gb|AAH08767.1|AAH08767 (BC008767) Similar to acyl-Coenzyme A oxidase 1, palmitoyl [Homo (E = 0.0)
Best antisense EST/protein match:BG431032 matched ref|XP_058817.1| (XM_058817) similar to putative (H. sapiens) [Homo sapiens] (E = 4e-66)

This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

  AI312541 cDNA clone IMAGE:1917620 kidney 3' read 0.4 kbProtein similarityPolyadenylation signal
  BG743305 cDNA clone IMAGE:4778447 skin 5' read Protein similarity
  AI302831 cDNA clone IMAGE:1900980 kidney 3' read 1.0 kbPolyadenylation signal
  AA863223 cDNA clone IMAGE:1455764 kidney 3' read Polyadenylation signal
  AI022322 cDNA clone IMAGE:1652263 pool 3' read 0.8 kbPolyadenylation signal
  AI033198 cDNA clone IMAGE:1654499 pool 3' read 0.7 kb
  BG431032 cDNA clone IMAGE:4612283 kidney 5' read
  BM015542 cDNA clone IMAGE:5417894 mammary adenocarcinoma, cell line 5' read
  BI192807 cDNA clone IMAGE:5088513 epithelioid carcinoma cell line 5' read
  BE797623 cDNA clone IMAGE:3938871 small cell carcinoma 5' read
  BI193083 cDNA clone IMAGE:5090722 epithelioid carcinoma cell line 5' read
  BG825104 cDNA clone IMAGE:4900290 rhabdomyosarcoma 5' read
  BI253773 cDNA clone IMAGE:5115365 choriocarcinoma 5' read
  BE798305 cDNA clone IMAGE:3938872 small cell carcinoma 5' read
  BF308054 cDNA clone IMAGE:4123424 rhabdomyosarcoma 5' read
  BG291518 cDNA clone IMAGE:4514717 transitional cell papilloma, cell line 5' read
  BG394244 cDNA clone IMAGE:4578827 retinoblastoma 5' read
  BI192782 cDNA clone IMAGE:5088505 epithelioid carcinoma cell line 5' read
  BE782476 cDNA clone IMAGE:3870913 retinoblastoma 5' read
  BG287063 cDNA clone IMAGE:4499870 transitional cell papilloma, cell line 5' read
  BM051009 cDNA clone IMAGE:5424571 normal pigmented retinal epithelium 5' read
  BF215728 cDNA clone IMAGE:4093900 glioblastoma 5' read
  BM053162 cDNA clone (no-name) islets of langerhans 5' read
  BM053101 cDNA clone (no-name) islets of langerhans 5' read
  AW249028 cDNA clone IMAGE:2820846 small cell carcinoma 5' read
  BM010433 cDNA clone IMAGE:5445160 amelanotic melanoma, cell line 5' read
  AA340111 cDNA clone ATCC:141765 skin 5' read
  AV691361 cDNA clone GKCEHE10 hepatocellular carcinoma 5' read


  BG474063 cDNA clone IMAGE:4648207 retinoblastoma 5' read Polyadenylation signal
  BE252740 cDNA clone IMAGE:3353560 retinoblastoma 5' read
  BE293358 cDNA clone IMAGE:3051501 adenocarcinoma cell line 5' read Protein similarity
  BG570439 cDNA clone IMAGE:4717367 lung 5' read Protein similarity
  BE253010 cDNA clone IMAGE:3357409 retinoblastoma 5' read Protein similarity
  AU076996 cDNA clone MKNa0041
  AU100165 cDNA clone MKNa0041 Protein similarity
  BG480783 cDNA clone IMAGE:4653551 choriocarcinoma 5' read Protein similarity
  AA376469 cDNA clone ATCC:180923 5' read Protein similarity
  BI490130 cDNA clone IMAGE:5173382 pooled brain, lung, testis 5' read Protein similarity
  AA380345 cDNA clone ATCC:184736 5' read Protein similarity