Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.115521
Description:REV3 (yeast homolog)-like, catalytic subunit of DNA polymerase zeta
Best-Of-UniGene (BOU) SequenceNM_002912
Genomic Coordiantes Displayed:Bases 2990000 to 3060000 of contig Hs6_7361_24
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingHs.115521.jpeg
Best sense EST/protein match:AI920786 matched ref|XP_040365.1| (XM_040365) REV3 (yeast homolog)-like, catalytic subunit of DNA (E = e-104)
Best antisense EST/protein match:No protein match with an e-value of less than 1e-10


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  BG393386 cDNA clone IMAGE:4540518 embryonal carcinoma, cell line 5' read Protein similarity
  BG547169 cDNA clone IMAGE:4702411 lung 5' read Protein similarity
  C14620 cDNA clone GEN076B05 aorta 5' read Protein similarity
  C14733 cDNA clone GEN084G11 aorta 5' read Protein similarity
  D61276 cDNA clone GEN174G08 brain 5' read Protein similarity
  D80654 cDNA clone GEN094C07 brain 5' read Protein similarity
  C15437 cDNA clone GEN145E11 aorta 5' read Protein similarity

SENSE ESTs

  BE884601 cDNA clone IMAGE:3908376 leiomyosarcoma 5' read Protein similarity
  AA197270 cDNA clone IMAGE:629419 muscle 5' read 1.5 kbProtein similarity
  BG255285 cDNA clone IMAGE:4478079 adenocarcinoma, cell line 5' read Protein similarity
  BF028969 cDNA clone IMAGE:3996756 carcinoma, cell line 5' read Protein similarity
  AW771677 cDNA clone IMAGE:3006482 2 pooled wilms' tumors, one primary and one metastatic to 3' read Protein similarity
  BE967481 cDNA clone IMAGE:3932873 brain 5' read Protein similarity
  AU117179 cDNA clone HEMBA1000833 whole embryo, mainly head 5' read Protein similarity
  AW771182 cDNA clone IMAGE:3027401 juvenile granulosa tumor 3' read Protein similarity
  AI920786 cDNA clone IMAGE:2452308 uterus 3' read 1.7 kbProtein similarity
  AU144077 cDNA clone HEMBA1000833 whole embryo, mainly head 3' read Protein similarity
  AA120977 cDNA clone IMAGE:548751 5' read 1.3 kbProtein similarity
  AA334113 cDNA clone ATCC:135782 whole embryo 5' read Protein similarity
  R12903 cDNA clone IMAGE:27443 brain 5' read 2.3 kbProtein similarity
  R34401 cDNA clone IMAGE:36986 brain 5' read 2.0 kbProtein similarity
  BE866859 cDNA clone IMAGE:3846858 adenocarcinoma 5' read Protein similarity
  AA310540 cDNA clone ATCC:156563 5' read Protein similarity
  AW958271 cDNA clone (no-name) Protein similarity
  BF210602 cDNA clone IMAGE:4097822 from chronic myelogenous leukemia 5' read Protein similarity
  BF977076 cDNA clone IMAGE:4305767 melanotic melanoma, high mdr 5' read Protein similarity
  BF208746 cDNA clone IMAGE:4093155 carcinoma, cell line 5' read Protein similarity
  BM068795 cDNA clone (no-name) islets of langerhans 5' read
  BE546837 cDNA clone IMAGE:3458943 choriocarcinoma 5' read Protein similarity
  AA308432 cDNA clone ATCC:112423 5' read
  BG403781 cDNA clone IMAGE:4526549 transitional cell papilloma, cell line 5' read
  BF107552 cDNA clone IMAGE:4043591 placenta 5' read
  BM054758 cDNA clone (no-name) islets of langerhans 3' read Polyadenylation signal
  BG236077 cDNA clone IMAGE:4141804 lymphocyte 3' read Polyadenylation signal
  AW194018 cDNA clone IMAGE:2683854 uterus 3' read Polyadenylation signal
  AI521064 cDNA clone IMAGE:2183637 stomach 3' read 0.9 kb
  N66749 cDNA clone IMAGE:284904 ear 3' read Polyadenylation signal
  R48939 cDNA clone IMAGE:36986 brain 3' read 2.0 kbPolyadenylation signal
  R40055 cDNA clone IMAGE:27443 brain 3' read 2.3 kbPolyadenylation signal
  R49301 cDNA clone IMAGE:36947 brain 3' read 2.1 kbPolyadenylation signal