Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.118821
Description:CGI-62 protein
Best-Of-UniGene (BOU) SequenceNM_016010
Genomic Coordiantes Displayed:Bases 715031 to 786093 of contig Hs8_8343_24
BOU Orientation Along Contig:RIGHT-TO-LEFT with respect to contig
Link to JPEG of genomic mappingHs.118821.jpeg
Best sense EST/protein match:AI074446 matched ref|NP_057094.1| (NM_016010) CGI-62 protein [Homo sapiens] (E = e-104)
Best antisense EST/protein match:BG924360 matched emb|CAC81058.1| (AJ271468) mitochondrial F1 ATP synthase beta subunit (E = 6e-11)

This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

  BG924360 cDNA clone (no-name) cartilage
  D59686 cDNA clone GEN056E09 brain 5' read
  D80220 cDNA clone GEN048D02 brain 5' read
  D59579 cDNA clone GEN047F01 brain 5' read
  D59819 cDNA clone GEN069A05 brain 5' read
  D59684 cDNA clone GEN056E08 brain 5' read
  D59731 cDNA clone GEN062B10 brain 5' read
  D59629 cDNA clone GEN052D06 brain 5' read
  D59642 cDNA clone GEN053D06 brain 5' read
  C14327 cDNA clone GEN046E03 aorta 5' read
  D59488 cDNA clone GEN040H10 brain 3' read
  D59818 cDNA clone GEN069A05 brain 3' read
  C14433 cDNA clone GEN056E08 aorta 3' read
  D59776 cDNA clone GEN065D12 brain 3' read
  D59685 cDNA clone GEN056E09 brain 3' read
  D59606 cDNA clone GEN049G08 brain 3' read
  D59597 cDNA clone GEN048G03 brain 3' read
  D59641 cDNA clone GEN053D06 brain 3' read
  D59594 cDNA clone GEN048D02 brain 3' read
  D59578 cDNA clone GEN047F01 brain 3' read
  D59628 cDNA clone GEN052D06 brain 3' read
  D59562 cDNA clone GEN046E03 brain 3' read
  C14473 cDNA clone GEN062B10 aorta 3' read


  AV741143 cDNA clone CBFAAD12 cord blood 5' read
  AL134560 cDNA clone DKFZp547A056 brain 5' read
  AA248785 cDNA clone (no-name) heart 5' read Protein similarity
  AA699423 cDNA clone IMAGE:433264 pool 3' read Protein similarity
  AI074446 cDNA clone IMAGE:1673690 pool 3' read 0.8 kbProtein similarity
  AI075776 cDNA clone IMAGE:1674718 pool 3' read 0.8 kbProtein similarity
  BI522663 cDNA clone IMAGE:5240252 brain 5' read
  AI984119 cDNA clone IMAGE:2562117 four pooled poorly-differentiated adenocarcinomas 3' read 1.1 kb
  W87505 cDNA clone IMAGE:416953 pool 3' read 0.8 kbProtein similarity
  BG571311 cDNA clone IMAGE:4719283 placenta 5' read
  AL110342 cDNA clone DKFZp564I0678 brain 5' read
  BG399858 cDNA clone IMAGE:4557929 kidney 5' read Protein similarity
  BI600862 cDNA clone IMAGE:5286549 hypothalamus, cell line 5' read Protein similarity
  BG426520 cDNA clone IMAGE:4607383 kidney 5' read Protein similarity
  BI824117 cDNA clone IMAGE:5180247 pooled brain, lung, testis 5' read Protein similarity
  BE779703 cDNA clone IMAGE:3868425 retinoblastoma 5' read Protein similarity
  BG545402 cDNA clone IMAGE:4700816 lung 5' read Protein similarity
  W87598 cDNA clone IMAGE:416953 pool 5' read 0.8 kbProtein similarity
  AU098372 cDNA clone CAS00707 Protein similarity