Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.12082
Best-Of-UniGene (BOU) SequenceAL117425
Genomic Coordiantes Displayed:Bases 395400 to 406600 of contig Hs5_6867_24
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingHs.12082.jpeg
Best sense EST/protein match:No protein match with an e-value of less than 1e-10
Best antisense EST/protein match:BG718944 matched ref|NP_443726.1| (NM_053000) TIGA1 [Homo sapiens] (E = 1e-47)

This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

  BG718944 cDNA clone IMAGE:4831353 testis, cell line 5' read
  AI936019 cDNA clone IMAGE:2452089 uterus 3' read 0.8 kbPolyadenylation signal
  BF969780 cDNA clone IMAGE:4360539 adrenal cortex carcinoma, cell line 5' read Polyadenylation signal
  BE395909 cDNA clone IMAGE:3659970 endometrium, adenocarcinoma cell line 5' read
  BE259773 cDNA clone IMAGE:3508639 neuroblastoma 5' read
  BE392562 cDNA clone IMAGE:3659969 endometrium, adenocarcinoma cell line 5' read
  AI269707 cDNA clone IMAGE:1998294 brain 3' read Polyadenylation signal
  AI269715 cDNA clone IMAGE:1998295 brain 3' read Polyadenylation signal
  AI472397 cDNA clone IMAGE:2152937 colon 3' read 2.1 kb
  BF680239 cDNA clone IMAGE:4296071 prostate 5' read
  BI334614 cDNA clone IMAGE:5141892 choriocarcinoma 5' read
  BI086626 cDNA clone IMAGE:4991815 placenta 5' read
  BI094245 cDNA clone IMAGE:5001127 placenta 5' read