Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.125819
Description:putative dimethyladenosine transferase
Best-Of-UniGene (BOU) SequenceNM_014473
Genomic Coordiantes Displayed:Bases 628000 to 642000 of contig Hs5_6844_24
BOU Orientation Along Contig:RIGHT-TO-LEFT with respect to contig
Link to JPEG of genomic mappingHs.125819.jpeg
Best sense EST/protein match:BE569090 matched ref|NP_055288.1| (NM_014473) putative dimethyladenosine transferase [Homo sapiens] (E = e-102)
Best antisense EST/protein match:No protein match with an e-value of less than 1e-10


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  F11417 cDNA clone c2wg02 brain
  AA383883 cDNA clone ATCC:188045 thymus 5' read
  BE538333 cDNA clone IMAGE:3454710 placenta 5' read
  BG501822 cDNA clone IMAGE:4654900 embryonal carcinoma 5' read
  BI092489 cDNA clone IMAGE:4997828 placenta 5' read
  BG621581 cDNA clone IMAGE:4731019 placenta 5' read
  AA004948 cDNA clone IMAGE:428302 pool 5' read 0.9 kb
  BF126573 cDNA clone IMAGE:4071607 liver 5' read
  BG399336 cDNA clone IMAGE:4557424 kidney 5' read
  W87858 cDNA clone IMAGE:417220 pool 5' read 0.7 kb
  AA004949 cDNA clone IMAGE:428302 pool 3' read 0.9 kb
  W87688 cDNA clone IMAGE:417220 pool 3' read 0.7 kbPolyadenylation signal
  AW150619 cDNA clone IMAGE:2632140 uterus 3' read Polyadenylation signal
  F09084 cDNA clone c2wg02 brain 3' read Polyadenylation signal
  BG034799 cDNA clone IMAGE:4413514 adenocarcinoma, cell line 5' read
  AW510477 cDNA clone IMAGE:2681804 uterus 3' read Polyadenylation signal
  AW084822 cDNA clone IMAGE:2588970 esophagus 3' read Polyadenylation signal
  AI624532 cDNA clone IMAGE:2228903 pancreas 3' read 1.3 kb
  BG496531 cDNA clone IMAGE:4672309 mucoepidermoid carcinoma 5' read
  AW512269 cDNA clone IMAGE:2849193 lymph 3' read
  R54241 cDNA clone IMAGE:39258 brain 5' read 1.6 kb
  R21562 cDNA clone IMAGE:31387 brain 5' read 1.6 kb
  BE748238 cDNA clone IMAGE:3838360 from acute myelogenous leukemia 5' read
  BF217993 cDNA clone IMAGE:4095184 glioblastoma 5' read
  BF107099 cDNA clone IMAGE:4043462 placenta 5' read
  BF028595 cDNA clone IMAGE:3995861 carcinoma, cell line 5' read
  BE785091 cDNA clone IMAGE:3881221 large cell carcinoma 5' read
  W47058 cDNA clone IMAGE:325034 5' read 0.8 kb
  AW021032 cDNA clone IMAGE:2483948 ear 5' read
  BE748533 cDNA clone IMAGE:3838360 from acute myelogenous leukemia 3' read
  BE281129 cDNA clone IMAGE:3504623 choriocarcinoma 5' read Protein similarity
  BE042735 cDNA clone IMAGE:3038624 colon 3' read Protein similarityPolyadenylation signal
  AW518132 cDNA clone IMAGE:2850071 lymph 3' read Protein similarityPolyadenylation signal
  R51847 cDNA clone IMAGE:39258 brain 3' read 1.6 kbProtein similarityPolyadenylation signal
  W46939 cDNA clone IMAGE:325034 3' read 0.8 kbProtein similarityPolyadenylation signal

SENSE ESTs

  AW166907 cDNA clone IMAGE:2633476 uterus 3' read
  AI040003 cDNA clone IMAGE:1664280 3' read 1.5 kbProtein similarityPolyadenylation signal
  BG779590 cDNA clone IMAGE:4808239 adenocarcinoma 5' read
  BG499952 cDNA clone IMAGE:4667622 adenocarcinoma 5' read
  BG258875 cDNA clone IMAGE:4509780 embryonal carcinoma, cell line 5' read Protein similarity
  BG574172 cDNA clone IMAGE:4704877 mammary adenocarcinoma, cell line 5' read
  AW272926 cDNA clone IMAGE:2818849 two pooled squamous cell carcinomas 3' read
  BG178374 cDNA clone IMAGE:4431419 adenocarcinoma, cell line 5' read Protein similarity
  BG494859 cDNA clone IMAGE:4671713 mucoepidermoid carcinoma 5' read Protein similarity
  BE569090 cDNA clone IMAGE:3681574 carcinoma, cell line 5' read Protein similarity
  BI823552 cDNA clone IMAGE:5181795 pooled brain, lung, testis 5' read Protein similarity
  AV716100 cDNA clone DCBBLA11 5' read
  BG620520 cDNA clone IMAGE:4731473 placenta 5' read Protein similarity
  BG106342 cDNA clone IMAGE:4384467 lymphoma, cell line 5' read Protein similarity
  AW439147 cDNA clone IMAGE:2778766 uterus 3' read Polyadenylation signal
  BE787291 cDNA clone IMAGE:3879886 large cell carcinoma 5' read Protein similarity
  BI667160 cDNA clone IMAGE:5310924 hypothalamus, cell line 5' read Protein similarity
  BF688159 cDNA clone IMAGE:4066390 glioblastoma 5' read Protein similarity
  BF025861 cDNA clone IMAGE:3953141 melanotic melanoma 5' read Protein similarity
  BF684683 cDNA clone IMAGE:4301964 leiomyosarcoma cell line 5' read Protein similarity
  BI767933 cDNA clone IMAGE:5210036 pooled lung and spleen 5' read Protein similarity
  BF218045 cDNA clone IMAGE:4095248 glioblastoma 5' read Protein similarity
  BI916814 cDNA clone IMAGE:5242042 brain 5' read Protein similarity
  BG289234 cDNA clone IMAGE:4513257 transitional cell papilloma, cell line 5' read Protein similarity
  BG323360 cDNA clone IMAGE:4559866 renal cell adenocarcinoma 5' read Protein similarity
  BG611677 cDNA clone IMAGE:4738780 adenocarcinoma 5' read Protein similarity
  BI559342 cDNA clone IMAGE:5295505 testis, cell line 5' read Protein similarity
  BI668024 cDNA clone IMAGE:5314909 hypothalamus, cell line 5' read Protein similarity
  BE409485 cDNA clone IMAGE:3638494 choriocarcinoma 5' read Protein similarity
  BM013225 cDNA clone IMAGE:5434356 neuroblastoma, cell line 5' read
  BM050162 cDNA clone IMAGE:5422253 epithelioid carcinoma cell line 5' read
  BE788748 cDNA clone IMAGE:3878818 large cell carcinoma 5' read Protein similarity
  BG531532 cDNA clone IMAGE:4697624 embryonal carcinoma 5' read Protein similarity
  BI910985 cDNA clone IMAGE:5218151 leukocyte 5' read Protein similarity
  BE390478 cDNA clone IMAGE:3605395 endometrium, adenocarcinoma cell line 5' read Protein similarity