Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.150601
Description:chymotrypsin-like
Best-Of-UniGene (BOU) SequenceAJ272212
Genomic Coordiantes Displayed:Bases 830381 to 885711 of contig Hs16_10635_24
BOU Orientation Along Contig:RIGHT-TO-LEFT with respect to contig
Link to JPEG of genomic mappingHs.150601.jpeg
Best sense EST/protein match:AJ272212 matched gb|AAL40735.1| (AF416988) protein serine kinase/luciferase fusion protein [synthetic (E = 0.0)
Best antisense EST/protein match:BI521033 matched ref|NP_001898.1| (NM_001907) chymotrypsin-like; Chymotrypsin-like protease [Homo (E = e-132)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  BI838514 cDNA clone IMAGE:5225162 pooled pancreas and spleen 5' read Protein similarity
  BI833975 cDNA clone IMAGE:5224196 pooled pancreas and spleen 5' read Protein similarity
  BI838556 cDNA clone IMAGE:5225188 pooled pancreas and spleen 5' read Protein similarity
  BI834199 cDNA clone IMAGE:5223073 pooled pancreas and spleen 5' read Protein similarity
  BI521986 cDNA clone IMAGE:5220749 pooled pancreas and spleen 3' read Protein similarity
  BE969629 cDNA clone IMAGE:3949886 pancreas 5' read Protein similarity
  BI836805 cDNA clone IMAGE:5224173 pooled pancreas and spleen 5' read Protein similarity
  BI832697 cDNA clone IMAGE:5221295 pooled pancreas and spleen 5' read Protein similarity
  BI832541 cDNA clone IMAGE:5221453 pooled pancreas and spleen 5' read Protein similarity
  BI835048 cDNA clone IMAGE:5228157 pooled pancreas and spleen 5' read Protein similarity
  BI836955 cDNA clone IMAGE:5223282 pooled pancreas and spleen 5' read Protein similarity
  BI521033 cDNA clone IMAGE:5220749 pooled pancreas and spleen 5' read Protein similarity
  BI837550 cDNA clone IMAGE:5225907 pooled pancreas and spleen 5' read Protein similarity
  BI832701 cDNA clone IMAGE:5221512 pooled pancreas and spleen 5' read Protein similarity
  BI835109 cDNA clone IMAGE:5226595 pooled pancreas and spleen 5' read Protein similarity
  BI836061 cDNA clone IMAGE:5223109 pooled pancreas and spleen 5' read Protein similarity
  AW874469 cDNA clone IMAGE:3122463 pancreas 3' read Protein similarity
  BM023178 cDNA clone (no-name) islets of langerhans 3' read
  BM023460 cDNA clone (no-name) islets of langerhans 5' read
  BM021468 cDNA clone (no-name) islets of langerhans 5' read
  BM021196 cDNA clone (no-name) islets of langerhans 3' read
  AA844904 cDNA clone IMAGE:1411967 pancreas 3' read Polyadenylation signal
  BM053138 cDNA clone (no-name) islets of langerhans 5' read Polyadenylation signal
  BM052825 cDNA clone (no-name) islets of langerhans 3' read Polyadenylation signal

SENSE ESTs

  AI247458 cDNA clone IMAGE:1848666 pool 3' read 0.4 kb
  BG654996 cDNA clone (no-name) purified pancreatic islet 5' read
  BM128500 cDNA clone (no-name) islets of langerhans 3' read
  BM128873 cDNA clone (no-name) islets of langerhans 3' read
  BM129135 cDNA clone (no-name) islets of langerhans 5' read
  BE871741 cDNA clone IMAGE:3852203 adenocarcinoma 5' read
  BF445737 cDNA clone IMAGE:4091091 sympathetic trunk 3' read
  AA318171 cDNA clone ATCC:118648 eye 5' read
  AI985932 cDNA clone IMAGE:2562310 four pooled poorly-differentiated adenocarcinomas 3' read 1.2 kb
  BF688964 cDNA clone IMAGE:4299625 normal pigmented retinal epithelium 3' read
  C17934 cDNA clone GEN555A06 placenta 5' read
  C17257 cDNA clone GEN542D05 aorta 5' read
  BG757085 cDNA clone IMAGE:4855515 primary b-cells from tonsils (cell line) 5' read
  BG750582 cDNA clone IMAGE:4844835 normal pigmented retinal epithelium 5' read
  BF688492 cDNA clone IMAGE:4299625 normal pigmented retinal epithelium 5' read
  BG742657 cDNA clone IMAGE:4778438 skin 5' read
  AA378922 cDNA clone ATCC:183327 synovial membrane 5' read
  BI193705 cDNA clone IMAGE:5090149 epithelioid carcinoma cell line 5' read
  BG769288 cDNA clone IMAGE:4872558 melanotic melanoma, high mdr (cell line) 5' read
  BG820457 cDNA clone IMAGE:4933570 anaplastic oligodendroglioma with 1p/19q loss 5' read Protein similarity
  BE902568 cDNA clone IMAGE:3959715 choriocarcinoma 5' read
  AL134849 cDNA clone DKFZp762E2310 melanoma (mewo cell line) 5' read Protein similarity
  BI762611 cDNA clone IMAGE:5188431 pooled colon, kidney, stomach 5' read Protein similarity