Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.154057
Description:matrix metalloproteinase 19
Best-Of-UniGene (BOU) SequenceNM_022790
Genomic Coordiantes Displayed:Bases 1395000 to 1430000 of contig Hs12_9615_24
BOU Orientation Along Contig:RIGHT-TO-LEFT with respect to contig
Link to JPEG of genomic mappingHs.154057.jpeg
Best sense EST/protein match:BI762731 matched ref|NP_002420.1| (NM_002429) matrix metalloproteinase 19, isoform rasi-1, (E = e-144)
Best antisense EST/protein match:BI116725 matched ref|XP_071745.1| (XM_071745) hypothetical protein XP_071745 [Homo sapiens] (E = 7e-32)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  BG483291 cDNA clone IMAGE:4617637 lung 5' read
  BI762708 cDNA clone IMAGE:5188793 pooled colon, kidney, stomach 5' read
  BI757609 cDNA clone IMAGE:5199693 brain 5' read
  BF238265 cDNA clone IMAGE:4054828 primary b-cells from tonsils (cell line) 5' read
  Z45550 cDNA clone c2sb10 brain
  BI910370 cDNA clone IMAGE:5217007 leukocyte 5' read
  BI116725 cDNA clone IMAGE:5016891 small cell carcinoma 5' read
  BI754435 cDNA clone IMAGE:5193390 brain 5' read
  BI836674 cDNA clone IMAGE:5228569 pooled pancreas and spleen 5' read
  BF968189 cDNA clone IMAGE:4357132 adrenal cortex carcinoma, cell line 5' read
  BI833444 cDNA clone IMAGE:5226961 pooled pancreas and spleen 5' read
  BI523916 cDNA clone IMAGE:5201271 pooled lung and spleen 5' read
  BI831513 cDNA clone IMAGE:5166463 medulla 5' read
  BI768537 cDNA clone IMAGE:5206690 pooled lung and spleen 5' read
  BI768213 cDNA clone IMAGE:5206000 pooled lung and spleen 5' read
  BI767471 cDNA clone IMAGE:5206713 pooled lung and spleen 5' read
  BI753624 cDNA clone IMAGE:5194081 brain 5' read
  BE891194 cDNA clone IMAGE:3917093 melanotic melanoma 5' read
  BE907108 cDNA clone IMAGE:3902845 epithelioid carcinoma 5' read
  AA069963 cDNA clone IMAGE:530894 3' read Polyadenylation signal
  AW630135 cDNA clone IMAGE:2968965 2 pooled high-grade transitional cell tumors 5' read
  BF337535 cDNA clone IMAGE:4183530 glioblastoma with egfr amplification 5' read
  BG913873 cDNA clone IMAGE:4944478 anaplastic oligodendroglioma with 1p/19q loss 5' read
  BI908895 cDNA clone IMAGE:5215725 leukocyte 5' read
  BG035011 cDNA clone IMAGE:4413453 adenocarcinoma, cell line 5' read
  AW613864 cDNA clone IMAGE:2968965 2 pooled high-grade transitional cell tumors 3' read Polyadenylation signal
  AI539594 cDNA clone IMAGE:2191564 uterus 3' read 0.6 kbPolyadenylation signal
  Z41245 cDNA clone c2sb10 brain 3' read Polyadenylation signal
  BI524667 cDNA clone IMAGE:5201271 pooled lung and spleen 3' read
  BF343698 cDNA clone IMAGE:4151077 glioblastoma with egfr amplification 5' read
  AI524551 cDNA clone IMAGE:2135252 lymph 3' read Polyadenylation signal
  AW440021 cDNA clone IMAGE:2775854 uterus 3' read
  AI270208 cDNA clone IMAGE:1962747 colon 3' read 1.1 kbPolyadenylation signal
  AI620559 cDNA clone IMAGE:2258800 stomach 3' read 1.1 kbPolyadenylation signal
  BE139486 cDNA clone IMAGE:2760522 uterus 3' read Polyadenylation signal
  AI803905 cDNA clone IMAGE:2189930 uterus 3' read 2.0 kbPolyadenylation signal
  AI540444 cDNA clone IMAGE:2209722 uterus 3' read Polyadenylation signal

SENSE ESTs

  AW069111 cDNA clone HBMSC_cr41d08 bone 3' read Polyadenylation signal
  AI263317 cDNA clone IMAGE:2005457 pancreas 3' read
  AI682896 cDNA clone IMAGE:2323919 pancreas 3' read 0.5 kbPolyadenylation signal
  BM141713 cDNA clone (no-name) islets of langerhans 3' read Polyadenylation signal
  BM141709 cDNA clone (no-name) islets of langerhans 3' read Polyadenylation signal
  AI619886 cDNA clone IMAGE:2281508 uterus 3' read 1.7 kbPolyadenylation signal
  AW082534 cDNA clone IMAGE:2579930 esophagus 3' read Polyadenylation signal
  AI432692 cDNA clone IMAGE:2121203 lymph 3' read 0.4 kbPolyadenylation signal
  AI890321 cDNA clone IMAGE:2442565 uterus 3' read 2.4 kb
  AW339116 cDNA clone IMAGE:2879192 pancreas 3' read
  AA703579 cDNA clone IMAGE:450263 pool 3' read
  AA778742 cDNA clone IMAGE:452474 pool 3' read Polyadenylation signal
  BM141974 cDNA clone (no-name) islets of langerhans 5' read
  BM141980 cDNA clone (no-name) islets of langerhans 5' read
  AA366977 cDNA clone ATCC:171654 pancreas 5' read Protein similarity
  BM050729 cDNA clone IMAGE:5424003 normal pigmented retinal epithelium 5' read
  BG677340 cDNA clone IMAGE:4749085 squamous cell carcinoma 5' read Protein similarity
  BI761698 cDNA clone IMAGE:5186893 pooled colon, kidney, stomach 5' read Protein similarity
  BI771459 cDNA clone IMAGE:5209132 pooled lung and spleen 5' read Protein similarity
  BI762731 cDNA clone IMAGE:5188847 pooled colon, kidney, stomach 5' read Protein similarity
  BI770124 cDNA clone IMAGE:5202913 pooled lung and spleen 5' read Protein similarity
  AV691145 cDNA clone GKCBKG04 hepatocellular carcinoma 5' read Protein similarity
  AV691144 cDNA clone GKCBKG03 hepatocellular carcinoma 5' read Protein similarity
  AA382079 cDNA clone ATCC:186472 5' read