Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.15535
Description:Homo sapiens clone 24582 mRNA sequence
Best-Of-UniGene (BOU) SequenceAF070554
Genomic Coordiantes Displayed:Bases 55856 to 81032 of contig Hs1_28210_24
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingHs.15535.jpeg
Best sense EST/protein match:No protein match with an e-value of less than 1e-10
Best antisense EST/protein match:No protein match with an e-value of less than 1e-10


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  AI364190 cDNA clone IMAGE:1993031 uterus 3' read 1.5 kb
  AW662167 cDNA clone IMAGE:2972874 2 pooled high-grade transitional cell tumors 3' read
  BG770846 cDNA clone IMAGE:4839584 adenocarcinoma 5' read
  AV718371 cDNA clone FHTBAAF02 hypothalamus 5' read
  BG429664 cDNA clone IMAGE:4614951 kidney 5' read
  BM172365 cDNA clone IMAGE:4839584 adenocarcinoma 5' read
  AI434567 cDNA clone IMAGE:2133829 lymph 3' read 2.0 kb

SENSE ESTs

  BE395090 cDNA clone IMAGE:3632473 endometrium, adenocarcinoma cell line 5' read
  BE887569 cDNA clone IMAGE:3913375 leiomyosarcoma 5' read
  BF038018 cDNA clone IMAGE:3865158 adenocarcinoma 5' read
  N56379 cDNA clone JJ7491 heart 5' read
  AV702073 cDNA clone ADBCVC08 adrenal gland 5' read
  AV702072 cDNA clone ADBCVC07 adrenal gland 5' read
  BI856960 cDNA clone IMAGE:5393806 mammary adenocarcinoma, cell line 5' read
  AI476191 cDNA clone IMAGE:2044628 lymph 3' read Polyadenylation signal
  AW272545 cDNA clone IMAGE:2800774 colon 3' read Polyadenylation signal
  BE049366 cDNA clone IMAGE:2834762 pancreas 3' read Polyadenylation signal
  AA921885 cDNA clone IMAGE:1537015 kidney 3' read 1.1 kbPolyadenylation signal