Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.182698
Description:mitochondrial ribosomal protein L20
Best-Of-UniGene (BOU) SequenceNM_017971
Genomic Coordiantes Displayed:Bases 386000 to 414000 of contig Hs1_25791_24
BOU Orientation Along Contig:RIGHT-TO-LEFT with respect to contig
Link to JPEG of genomic mappingHs.182698.jpeg
Best sense EST/protein match:BE514404 matched dbj|BAB40849.1| (AB049644) mitochondrial ribosomal protein L20 (L20mt) [Homo (E = 2e-81)
Best antisense EST/protein match:No protein match with an e-value of less than 1e-10

This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

  R12225 cDNA clone IMAGE:26011 brain 5' read 1.9 kb
  BI767355 cDNA clone IMAGE:5206943 pooled lung and spleen 5' read
  BF037666 cDNA clone IMAGE:3865346 adenocarcinoma 5' read
  BI118146 cDNA clone IMAGE:5015766 small cell carcinoma 5' read
  AU143273 cDNA clone Y79AA1001665 5' read
  AW957724 cDNA clone (no-name)
  BG254416 cDNA clone IMAGE:4477107 adenocarcinoma, cell line 5' read
  BE905500 cDNA clone IMAGE:3897389 epithelioid carcinoma 5' read
  BE795124 cDNA clone IMAGE:3946646 small cell carcinoma 5' read
  BI517383 cDNA clone IMAGE:5162865 pooled colon, kidney, stomach 5' read
  BI758740 cDNA clone IMAGE:5200350 brain 5' read
  BI552758 cDNA clone IMAGE:5264910 hippocampus, cell line 5' read
  BI522617 cDNA clone IMAGE:5239952 brain 5' read
  AA206537 cDNA clone IMAGE:645819 5' read
  BI835129 cDNA clone IMAGE:5227704 pooled pancreas and spleen 5' read
  H06932 cDNA clone IMAGE:44541 brain 5' read 1.9 kb
  BG328690 cDNA clone IMAGE:4547511 adenocarcinoma cell line 5' read
  BI257793 cDNA clone IMAGE:5109283 choriocarcinoma 5' read
  BE788817 cDNA clone IMAGE:3878408 large cell carcinoma 5' read
  BE903333 cDNA clone IMAGE:3957148 choriocarcinoma 5' read
  BI911209 cDNA clone IMAGE:5212237 leukocyte 5' read
  BI838864 cDNA clone IMAGE:5221631 pooled pancreas and spleen 5' read