Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.184846
Description:Homo sapiens, Similar to zinc finger protein 304, clone MGC:4079 IMAGE:3530863, mRNA, complete cds
Best-Of-UniGene (BOU) SequenceBC005868
Genomic Coordiantes Displayed:Bases 94188 to 105101 of contig Hs19_11448_24
BOU Orientation Along Contig:RIGHT-TO-LEFT with respect to contig
Link to JPEG of genomic mappingHs.184846.jpeg
Best sense EST/protein match:BC005868 matched ref|XP_030206.1| (XM_030206) hypothetical protein XP_030206 [Homo sapiens] (E = 0.0)
Best antisense EST/protein match:No protein match with an e-value of less than 1e-10


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  F06052 cDNA clone c0vd03 brain
  Z43590 cDNA clone c1fg09 brain
  T26651 cDNA clone LLAB51G9 brain 5' read
  F02919 cDNA clone c1fg09 brain 3' read Protein similarity
  T26650 cDNA clone LLAB51G9 brain 3' read Protein similarity

SENSE ESTs

  AI689681 cDNA clone IMAGE:2269090 uterus 3' read 3.2 kb
  BE205780 cDNA clone IMAGE:3009681 rhabdomyosarcoma 3' read
  AW409872 cDNA clone IMAGE:2961247 rhabdomyosarcoma 3' read
  BG389860 cDNA clone IMAGE:4523634 embryonal carcinoma, cell line 5' read
  BE253245 cDNA clone IMAGE:3357543 retinoblastoma 5' read
  BE787290 cDNA clone IMAGE:3879838 large cell carcinoma 5' read Protein similarity
  BE790712 cDNA clone IMAGE:3936562 small cell carcinoma 5' read Protein similarity
  BF797956 cDNA clone IMAGE:4342146 lymphoma, cell line 5' read Protein similarity
  BG392721 cDNA clone IMAGE:4539810 embryonal carcinoma, cell line 5' read Protein similarity
  BE294508 cDNA clone IMAGE:3528996 rhabdomyosarcoma 5' read Protein similarity
  BE019037 cDNA clone IMAGE:3009681 rhabdomyosarcoma 5' read Protein similarity
  AA249462 cDNA clone (no-name) heart 5' read
  BE295522 cDNA clone IMAGE:3530863 rhabdomyosarcoma 5' read
  AA093691 cDNA clone (no-name) heart 5' read Protein similarity