Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.19545
Description:frizzled (Drosophila) homolog 4
Best-Of-UniGene (BOU) SequenceNM_012193
Genomic Coordiantes Displayed:Bases 5350000 to 5700000 of contig Hs11_9341_24
BOU Orientation Along Contig:RIGHT-TO-LEFT with respect to contig
Link to JPEG of genomic mappingHs.19545.jpeg
Best sense EST/protein match:BI757553 matched ref|NP_036325.1| (NM_012193) frizzled homolog 4 (Drosophila); frizzled (Drosophila) (E = e-124)
Best antisense EST/protein match:No protein match with an e-value of less than 1e-10

This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

  BI914433 cDNA clone IMAGE:5246259 brain 5' read Protein similarity
  BI818443 cDNA clone IMAGE:5174092 pooled brain, lung, testis 5' read Protein similarity
  BG574942 cDNA clone IMAGE:4706743 mammary adenocarcinoma, cell line 5' read Protein similarity
  AL599187 cDNA clone DKFZp313P1924 5' read
  AL599189 cDNA clone DKFZp313P2024 5' read Protein similarity
  AW069826 cDNA clone HBMSC_cr52b03 bone 3' read
  AA902950 cDNA clone IMAGE:1516769 smooth muscle 3' read 0.6 kbProtein similarity


  H17904 cDNA clone IMAGE:50620 brain 3' read 0.6 kbPolyadenylation signal
  BI495375 cDNA clone IMAGE:2539916 ear 5' read Polyadenylation signal
  BI495374 cDNA clone IMAGE:2539916 ear 3' read Polyadenylation signal
  BE968690 cDNA clone IMAGE:3933940 heart 5' read
  AI085074 cDNA clone IMAGE:1653956 pool 3' read 1.3 kbPolyadenylation signal
  AA937347 cDNA clone IMAGE:1491534 skin 3' read 1.0 kbPolyadenylation signal
  AA953349 cDNA clone IMAGE:1573258 kidney 3' read 0.7 kbPolyadenylation signal
  AA886232 cDNA clone IMAGE:1492849 kidney 3' read 0.7 kbPolyadenylation signal
  AI249934 cDNA clone IMAGE:2000841 lymph 3' read 1.9 kbPolyadenylation signal
  BI963125 cDNA clone (no-name) islets of langerhans 3' read Polyadenylation signal
  AV709171 cDNA clone ADCAVG12 adrenal gland 5' read Polyadenylation signal
  AA677200 cDNA clone IMAGE:454698 pool 3' read Polyadenylation signal
  AA011622 cDNA clone IMAGE:429599 pool 3' read 1.3 kbPolyadenylation signal
  AW029374 cDNA clone IMAGE:2543194 stomach 3' read 0.7 kbPolyadenylation signal
  AA745694 cDNA clone IMAGE:1323968 kidney 3' read 0.7 kbPolyadenylation signal
  H14866 cDNA clone IMAGE:49122 brain 5' read 0.6 kb
  AW591175 cDNA clone IMAGE:2703691 uterus 3' read
  N83945 cDNA clone KK4065 heart 5' read