Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.20621
Description:gamma tubulin ring complex protein (76p gene)
Best-Of-UniGene (BOU) SequenceAK026551
Genomic Coordiantes Displayed:Bases 5247780 to 5296735 of contig Hs15_10351_24
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingHs.20621.jpeg
Best sense EST/protein match:AU132686 matched ref|NP_055259.1| (NM_014444) gamma tubulin ring complex protein (76p gene) [Homo (E = e-118)
Best antisense EST/protein match:No protein match with an e-value of less than 1e-10


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  AI590739 cDNA clone IMAGE:2260029 tumor, 5 pooled (see description) 3' read 0.9 kb
  AI581561 cDNA clone IMAGE:2173100 colon 3' read Polyadenylation signal
  BF589795 cDNA clone IMAGE:3394086 sciatic nerve 3' read Polyadenylation signal
  R42295 cDNA clone IMAGE:29600 brain 3' read 1.7 kbPolyadenylation signal
  R45013 cDNA clone IMAGE:34314 brain 3' read 1.4 kbPolyadenylation signal
  R46065 cDNA clone IMAGE:35920 brain 3' read Polyadenylation signal
  R51230 cDNA clone IMAGE:38514 brain 3' read 1.7 kbPolyadenylation signal
  W45573 cDNA clone IMAGE:323476 3' read 1.2 kbPolyadenylation signal
  AI868142 cDNA clone IMAGE:2186145 stomach 3' read 2.5 kbPolyadenylation signal
  BG820636 cDNA clone IMAGE:4933879 anaplastic oligodendroglioma with 1p/19q loss 5' read
  W45624 cDNA clone IMAGE:323476 5' read 1.2 kb
  BG684200 cDNA clone IMAGE:4763671 primary b-cells from tonsils (cell line) 5' read
  R25005 cDNA clone IMAGE:34314 brain 5' read 1.4 kb
  AW957343 cDNA clone (no-name) Protein similarity
  R51229 cDNA clone IMAGE:38514 brain 5' read 1.7 kbProtein similarity
  R20563 cDNA clone IMAGE:35920 brain 5' read Protein similarity
  R16347 cDNA clone IMAGE:29600 brain 5' read 1.7 kb
  BF791920 cDNA clone IMAGE:4344573 adrenal cortex carcinoma, cell line 5' read
  BI755178 cDNA clone IMAGE:5193827 brain 5' read
  AA332139 cDNA clone ATCC:133783 whole embryo 5' read

SENSE ESTs

  BI600196 cDNA clone IMAGE:5287968 hypothalamus, cell line 5' read Protein similarity
  BI596858 cDNA clone IMAGE:5285799 hypothalamus, cell line 5' read
  AW250598 cDNA clone IMAGE:2821891 small cell carcinoma 5' read
  BE206999 cDNA clone IMAGE:2823687 small cell carcinoma 5' read Protein similarity
  BI089598 cDNA clone IMAGE:4996025 placenta 5' read Protein similarity
  BE902157 cDNA clone IMAGE:3959078 choriocarcinoma 5' read
  AA115395 cDNA clone IMAGE:511678 5' read 2.6 kbProtein similarity
  BI915473 cDNA clone IMAGE:5241697 brain 5' read
  BG719078 cDNA clone IMAGE:4831176 testis, cell line 5' read Protein similarity
  BI770172 cDNA clone IMAGE:5202885 pooled lung and spleen 5' read
  BE790985 cDNA clone IMAGE:3940649 small cell carcinoma 5' read
  BI460506 cDNA clone IMAGE:5267187 testis, cell line 5' read Protein similarity
  BG388053 cDNA clone IMAGE:4521672 embryonal carcinoma, cell line 5' read Protein similarity
  BE895163 cDNA clone IMAGE:3921095 melanotic melanoma 5' read Protein similarity
  BE535713 cDNA clone IMAGE:3446803 placenta 5' read Protein similarity
  BI116738 cDNA clone IMAGE:5016847 small cell carcinoma 5' read
  AU132686 cDNA clone NT2RP4000355 5' read Protein similarity
  BE746238 cDNA clone IMAGE:3928420 adenocarcinoma cell line 5' read Protein similarity
  AI702056 cDNA clone IMAGE:2209412 uterus 3' read 1.9 kb
  BG292465 cDNA clone IMAGE:4515599 transitional cell papilloma, cell line 5' read Protein similarity
  BE901051 cDNA clone IMAGE:3956996 choriocarcinoma 5' read
  BF035249 cDNA clone IMAGE:3860625 adenocarcinoma 5' read Protein similarity
  AA247967 cDNA clone (no-name) heart 5' read Protein similarity
  AA352362 cDNA clone ATCC:152554 5' read Protein similarity
  AW957284 cDNA clone (no-name) Protein similarity
  BE315167 cDNA clone IMAGE:3140735 adenocarcinoma cell line 5' read Protein similarity
  BE301007 cDNA clone IMAGE:2906900 choriocarcinoma 3' read Protein similarityPolyadenylation signal
  AA115396 cDNA clone IMAGE:511678 3' read 2.6 kbProtein similarityPolyadenylation signal
  W85911 cDNA clone IMAGE:415677 pool 3' read 1.2 kbProtein similarityPolyadenylation signal
  BI092182 cDNA clone IMAGE:4997741 placenta 5' read
  BI256380 cDNA clone IMAGE:5114395 choriocarcinoma 5' read Protein similarity
  BG179258 cDNA clone IMAGE:4432309 adenocarcinoma, cell line 5' read Protein similarity
  AI478722 cDNA clone IMAGE:2155348 colon 3' read 1.8 kb
  AW247338 cDNA clone IMAGE:2819286 small cell carcinoma 3' read
  AU154174 cDNA clone NT2RP4000355 3' read