Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.211601
Description:mitogen-activated protein kinase kinase kinase 12
Best-Of-UniGene (BOU) SequenceNM_006301
Genomic Coordiantes Displayed:Bases 14000 to 56000 of contig Hs12_9720_24
BOU Orientation Along Contig:RIGHT-TO-LEFT with respect to contig
Link to JPEG of genomic mappingHs.211601.jpeg
Best sense EST/protein match:BG683294 matched ref|XP_006800.3| (XM_006800) mitogen-activated protein kinase kinase kinase 12 [Homo (E = e-111)
Best antisense EST/protein match:No protein match with an e-value of less than 1e-10

This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

  BF337583 cDNA clone IMAGE:4183281 glioblastoma with egfr amplification 5' read
  AI061157 cDNA clone IMAGE:1699674 kidney 3' read
  AW339716 cDNA clone IMAGE:2919367 myeloid cells, 18 pooled cml cases, bcr/abl rearrangement 3' read
  AW631457 cDNA clone IMAGE:2970010 2 pooled high-grade transitional cell tumors 5' read
  AW082549 cDNA clone IMAGE:2579766 esophagus 3' read
  AA053674 cDNA clone IMAGE:510396 3' read 1.1 kb
  AI282377 cDNA clone IMAGE:1980548 kidney 3' read 1.2 kb
  AW627654 cDNA clone IMAGE:2970010 2 pooled high-grade transitional cell tumors 3' read
  AI086971 cDNA clone IMAGE:1680137 3' read 0.8 kb
  BG111876 cDNA clone IMAGE:4370948 osteosarcoma, cell line 5' read
  BI259474 cDNA clone IMAGE:5109601 choriocarcinoma 5' read
  AI871927 cDNA clone IMAGE:2439680 uterus 3' read 2.1 kb
  AI805426 cDNA clone IMAGE:2277428 uterus 3' read 0.5 kb
  AA676357 cDNA clone IMAGE:431587 pool 3' read
  AI051564 cDNA clone IMAGE:1673768 pool 3' read 1.0 kb
  AW957619 cDNA clone (no-name) Protein similarity
  BG759751 cDNA clone IMAGE:4851397 primary b-cells from tonsils (cell line) 5' read Protein similarity