Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.2182
Description:pro-melanin-concentrating hormone
Best-Of-UniGene (BOU) SequenceNM_002674
Genomic Coordiantes Displayed:Bases 904000 to 946000 of contig Hs12_9900_24
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingHs.2182.jpeg
Best sense EST/protein match:NM_002674 matched ref|NP_002665.1| (NM_002674) pro-melanin-concentrating hormone [Homo sapiens] (E = 2e-90)
Best antisense EST/protein match:BI916249 matched ref|XP_006589.1| (XM_006589) hypothetical protein FLJ20641 [Homo sapiens] (E = 1e-45)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  BF217475 cDNA clone IMAGE:4104350 glioblastoma 5' read Protein similarity
  BG168599 cDNA clone IMAGE:4455383 hypernephroma, cell line 5' read Protein similarity
  BE538546 cDNA clone IMAGE:3451706 placenta 5' read Protein similarity
  AI654739 cDNA clone IMAGE:2216389 ovary 3' read 0.6 kbPolyadenylation signal
  BG286475 cDNA clone IMAGE:4500663 transitional cell papilloma, cell line 5' read Protein similarity
  BG105175 cDNA clone IMAGE:4422675 adrenal cortex carcinoma, cell line 5' read Protein similarity
  BG493758 cDNA clone IMAGE:4673043 mucoepidermoid carcinoma 5' read Protein similarity
  BF029183 cDNA clone IMAGE:3996887 carcinoma, cell line 5' read Protein similarity
  BF208223 cDNA clone IMAGE:4092169 carcinoma, cell line 5' read Protein similarity
  BE567081 cDNA clone IMAGE:3683483 carcinoma, cell line 5' read Protein similarity
  BF211472 cDNA clone IMAGE:4046722 from chronic myelogenous leukemia 5' read
  BF699297 cDNA clone IMAGE:4282647 primitive neuroectoderm 5' read
  BI916249 cDNA clone IMAGE:5242501 brain 5' read

SENSE ESTs

  BI597555 cDNA clone IMAGE:5294709 hypothalamus, cell line 5' read Protein similarity
  BG706895 cDNA clone IMAGE:4794545 hypothalamus, cell line 5' read Protein similarity
  BG706782 cDNA clone IMAGE:4794845 hypothalamus, cell line 5' read Protein similarity
  BI603807 cDNA clone IMAGE:5286979 hypothalamus, cell line 5' read Protein similarity
  AV701768 cDNA clone ADBBQB10 adrenal gland 5' read Protein similarity
  AV708684 cDNA clone ADCAFH06 adrenal gland 5' read Protein similarity