Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.22116
Description:CDC14 (cell division cycle 14, S. cerevisiae) homolog B
Best-Of-UniGene (BOU) SequenceNM_033332
Genomic Coordiantes Displayed:Bases 1874000 to 1986000 of contig Hs9_8633_24
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingHs.22116.jpeg
Best sense EST/protein match:AU119655 matched gb|AAD15415.1| (AC006024) similar to Cdc14B1 phosphatase; similar to AF064104 (E = e-107)
Best antisense EST/protein match:No protein match with an e-value of less than 1e-10


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  AI889924 cDNA clone IMAGE:2440789 uterus 3' read 2.4 kbPolyadenylation signal
  Z38405 cDNA clone c0ab04 brain 3' read Polyadenylation signal
  AA705526 cDNA clone IMAGE:462356 pool 3' read Polyadenylation signal
  BG031076 cDNA clone IMAGE:4393422 mammary adenocarcinoma, cell line 5' read
  BG913316 cDNA clone IMAGE:4944223 anaplastic oligodendroglioma with 1p/19q loss 5' read
  AA904204 cDNA clone IMAGE:1440446 peripheral nervous system 3' read 0.7 kbPolyadenylation signal

SENSE ESTs

  AA305458 cDNA clone ATCC:128915 5' read Protein similarity
  BF979268 cDNA clone IMAGE:4374168 testis, cell line 5' read Protein similarity
  BG709497 cDNA clone IMAGE:4797211 hypothalamus, cell line 5' read Protein similarity
  BI667222 cDNA clone IMAGE:5311664 hypothalamus, cell line 5' read
  AU133076 cDNA clone NT2RP4001211 5' read Protein similarity
  AU133642 cDNA clone OVARC1000338 ovary, tumor tissue 5' read Protein similarity
  AU119655 cDNA clone HEMBA1006337 whole embryo, mainly head 5' read Protein similarity
  BE781072 cDNA clone IMAGE:3872221 retinoblastoma 5' read Protein similarity
  BI715316 cDNA clone (no-name) purified pancreatic islet 5' read
  AU154886 cDNA clone OVARC1000338 ovary, tumor tissue 3' read
  AU145941 cDNA clone HEMBA1006337 whole embryo, mainly head 3' read
  BI714758 cDNA clone (no-name) purified pancreatic islet 3' read
  BM127513 cDNA clone (no-name) islets of langerhans 5' read
  BM127224 cDNA clone (no-name) islets of langerhans 3' read
  AI682948 cDNA clone IMAGE:2262846 uterus 3' read 1.7 kb
  BG252399 cDNA clone IMAGE:4474198 adenocarcinoma, cell line 5' read
  AV688143 cDNA clone GKCGMH10 hepatocellular carcinoma 5' read
  AA204912 cDNA clone IMAGE:646090 5' read
  BI596722 cDNA clone IMAGE:5285352 hypothalamus, cell line 5' read
  BG708333 cDNA clone IMAGE:4795192 hypothalamus, cell line 5' read
  AA307744 cDNA clone ATCC:109266 5' read
  R58429 cDNA clone G3621 heart 5' read
  N86760 cDNA clone L0572 heart 5' read
  AV707305 cDNA clone ADBAJH03 adrenal gland 5' read
  AL119559 cDNA clone DKFZp761C0823 amygdala 5' read
  AA319686 cDNA clone ATCC:120239 adrenal gland 5' read
  BE540095 cDNA clone IMAGE:3447238 placenta 5' read
  T35321 cDNA clone ATCC:106549 prostate 5' read
  T35357 cDNA clone ATCC:106592 prostate 5' read
  AW957406 cDNA clone (no-name)
  BF130437 cDNA clone IMAGE:4044198 hypernephroma 5' read
  AL039953 cDNA clone DKFZp434J2212 testis 5' read
  Z43835 cDNA clone c1ma06 brain
  F07307 cDNA clone c21g06 brain
  BM142002 cDNA clone (no-name) islets of langerhans 5' read
  BG167198 cDNA clone IMAGE:4455176 hypernephroma, cell line 5' read
  BF792631 cDNA clone IMAGE:4346254 adrenal cortex carcinoma, cell line 5' read
  AA383343 cDNA clone ATCC:187529 testis 5' read
  AA777790 cDNA clone IMAGE:448939 pool 3' read Polyadenylation signal
  AI750036 cDNA clone IMAGE:2373960 colon 3' read Polyadenylation signal
  AU154480 cDNA clone NT2RP4001211 3' read Polyadenylation signal
  AI922319 cDNA clone IMAGE:2453156 uterus 3' read Polyadenylation signal
  AW021718 cDNA clone IMAGE:2484532 ear 5' read
  BI492655 cDNA clone IMAGE:2484532 ear 3' read Polyadenylation signal
  AI369003 cDNA clone IMAGE:1991371 uterus 3' read 0.8 kbPolyadenylation signal
  D62485 cDNA clone GEN290D03 aorta 5' read
  Z39900 cDNA clone c1ma06 brain 3' read Polyadenylation signal
  R43365 cDNA clone IMAGE:32365 brain 3' read 1.6 kbPolyadenylation signal